A5027909019- Reproductive Biology and Fertility
- Sperm and Testicular Function
- Ovarian function and disorders
- Advanced Fluorescence Microscopy Techniques
- Reproductive Health and Technologies
- Reproductive System and Pregnancy
- Plant Reproductive Biology
- Birth, Development, and Health
- Epigenetics and DNA Methylation
- Assisted Reproductive Technology and Twin Pregnancy
- Amphibian and Reptile Biology
- Urologic and reproductive health conditions
- Pluripotent Stem Cells Research
- Reproductive Physiology in Livestock
- Seed Germination and Physiology
- Urological Disorders and Treatments
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Reproductive biology and impacts on aquatic species
- Fibroblast Growth Factor Research
- Ion channel regulation and function
- Animal Genetics and Reproduction
- Hormonal and reproductive studies
- Animal Behavior and Reproduction
- Microtubule and mitosis dynamics
- Advanced Electron Microscopy Techniques and Applications
Experimental Medicine and Biology Institute
2016-2025
Consejo Nacional de Investigaciones Científicas y Técnicas
2015-2024
Yale University
2024
Colorado State University
2024
Institute of Astronomy and Space Physics
2005-2020
Centro de Educación Médica e Investigaciones Clínicas Norberto Quirno
2013
University of Pennsylvania
2007-2011
Center for Human Reproduction
2008-2009
University of Calabria
1990-2009
Instituto de Química y Fisicoquímica Biológicas
2005
Abstract The resolution of fluorescence microscopy images is limited by the physical properties light. In last decade, numerous super-resolution (SRM) approaches have been proposed to deal with such hindrance. Here we present Mean-Shift Super Resolution (MSSR), a new SRM algorithm based on Mean Shift theory, which extends spatial single beyond diffraction limit MSSR works low and high fluorophore densities, not architecture optical setup applicable as well temporal series. theoretical...
TFIID is a general transcription factor required for of most protein-coding genes by RNA polymerase II. TAF7L an X-linked germ cell-specific paralogue TAF7, which generally expressed component TFIID. Here, we report the generation Taf7l mutant mice homologous recombination in embryonic stem cells using Cre-loxP strategy. While spermatogenesis was completed Taf7l(-/Y) mice, weight testis decreased and amount sperm epididymides sharply reduced. Mutant epididymal exhibited abnormal morphology,...
The mechanisms involved in the regulation of mammalian sperm motility are not well understood. Calcium ions (Ca2+) have been suggested to play a key role maintenance motility; nevertheless, how Ca2+ modulates this process has yet completely characterized. can bind calmodulin and complex regulates activity multiple enzymes, including Ca2+/calmodulin-dependent protein kinases (CaM kinases). Results from study confirmed that presence incubation medium is essential for maintaining human...
Although recent evidence indicates that several chemokines and defensins, well-known as inflammatory mediators, are expressed in the male female reproductive tracts, location functional significance of chemokine networks sperm physiology tract interactions poorly understood. To address this deficiency our knowledge, we examined expression function CCR6, a receptor common to chemoattractant peptides, screened fluids for presence specific ligands. CCR6 protein is present mouse human mainly...
In mammalian fertilization, sperm-zona pellucida binding is considered to be a critical aspect of gamete interaction. this study, we examine the mouse sperm acrosomal matrix protein zona 3 receptor (ZP3R; formerly called sp56) because our interest in defining function matrix, particulate compartment within secretory acrosome. Using targeted deletion Zp3r gene by homologous recombination, examined fertility nullizygous animals. Our experiments showed that males and females homozygous for...
Oocyte activation at fertilization is brought about by the testis-specific phospholipase C zeta (PLCZ), owing to its ability induce oscillations in intracellular Ca(2+) concentration ([Ca(2+)](i)). Whereas this a highly conserved mechanism among mammals, important species-specific differences PLCZ sequence, activity, and expression have been reported. Thus, objectives of research were clone characterize Ca(2+)-releasing activity equine sperm testis. Molecular cloning yielded 1914-bp sequence...
Mammalian sperm require to spend a limited period of time in the female reproductive tract become competent fertilize process called capacitation. It is well established that HCO3- essential for capacitation because it activates atypical soluble adenylate cyclase ADCY10 leading cAMP production, and promotes alkalinization cytoplasm, membrane hyperpolarization. However, how transported into not understood. There evidence CFTR activity involved human but this channel integrated complex...
ABSTRACT Conception in mammals is determined by the fusion of a sperm cell with an oocyte during fertilization. Motility one features that allows them to succeed fertilization, and their flagellum essential for this function. Longitudinally, can be divided into midpiece, principal piece end piece. A precise cytoskeletal architecture tail key acquisition fertilization competence. It has been proposed actin cytoskeleton plays roles regulation motility; however, organization remains elusive. In...
Cyclic adenosine monophosphate (cAMP) plays a central role in sperm physiology. Various cAMP up-regulators, both analogs and phosphodiesterase (PDE) inhibitors, have been used handling human vitro, though conflicting results variable responses among patients reported. This study aims to evaluate the ability of two compounds - Sp-5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole 3’-5’-cyclic monophosphothioate (cBiMPs), analog, TAK-063, specific PDE10A inhibitor enhance motility parameters...
Human semen is composed of a heterogeneous population sperm with varying degrees structural and functional differentiation normality, which result in subpopulations different quality.Using discontinuous Percoll gradient, we separated three subsets [(45%; L45), (65%; L65) (90%; L90) fractions] from normozoospermic human samples healthy donors proceeded to characterize their morphology, motility hyperactivation, as well ability undergo tyrosine phosphorylation under capacitating conditions.As...
Poor-quality sperm show reduced capacity to undergo capacitation-induced protein tyrosine phosphorylation and hyperactivation. Given that these deficiencies can be overcome by membrane-permeant stimulators of the cAMP-dependent kinase system, we hypothesize main defect underlying resides on plasma membrane. Spermatozoa from semen samples obtained 15 consenting healthy donors were separated in 2 subpopulations, L45 (first interface) L90 (pellet), using a 45:65:90 ISolate gradient...
To fertilize an egg, sperm must reside in the female reproductive tract to undergo several maturational changes that are collectively referred as capacitation. From a molecular point of view, HCO3−-dependent activation atypical soluble adenylyl cyclase (ADCY10) is one first events occurs during capacitation and leads subsequent cAMP-dependent protein kinase A (PKA). Capacitation also accompanied by hyperpolarization plasma membrane. We previously reported PKA necessary for CFTR (cystic...
During capacitation, sperm acquire the ability to undergo acrosome reaction (AR), an essential step in fertilization. Progesterone produced by cumulus cells has been associated with various physiological processes sperm, including stimulation of AR. An increase intracellular Ca(2+) ([Ca(2+)]i) is necessary for AR occur. In this study, we investigated spatiotemporal correlation between changes [Ca(2+)]i and single mouse spermatozoa response progesterone. We found that progesterone stimulates...