A5022967700- Advanced Fluorescence Microscopy Techniques
- Cell Image Analysis Techniques
- Advanced Electron Microscopy Techniques and Applications
- HIV Research and Treatment
- Health and Medical Research Impacts
- Biomedical and Engineering Education
- Global Health and Surgery
- Cytomegalovirus and herpesvirus research
- Mitochondrial Function and Pathology
- Genetics, Bioinformatics, and Biomedical Research
- Advanced Optical Sensing Technologies
- Advanced X-ray and CT Imaging
- Spectroscopy Techniques in Biomedical and Chemical Research
- Immune Response and Inflammation
- Infectious Diseases and Tuberculosis
- Diversity and Career in Medicine
- Force Microscopy Techniques and Applications
- Metabolism and Genetic Disorders
- Photoacoustic and Ultrasonic Imaging
- Immune cells in cancer
- Immunotherapy and Immune Responses
- Orthopedic Infections and Treatments
- Alzheimer's disease research and treatments
- Virus-based gene therapy research
- Antimicrobial Resistance in Staphylococcus
University of Cape Town
2018-2024
Wellcome Centre for Infectious Diseases Research in Africa
2020-2023
South African Medical Research Council
2020-2021
MRC Laboratory for Molecular Cell Biology
2017-2021
University College London
2017-2021
Education Labour Relations Council
2014
Abstract In the dynamic landscape of scientific research, imaging core facilities are vital hubs propelling collaboration and innovation at technology development dissemination frontier. Here, we present a collaborative effort led by Global BioImaging (GBI), introducing international recommendations geared towards elevating careers Imaging Scientists in facilities. Despite critical role modern research ecosystems, challenges persist recognising their value, aligning performance metrics...
Single-molecule localization microscopy (SMLM) techniques allow near molecular scale resolution ($\sim$ 20nm) as well precise and robust analysis of protein organization at different scales. SMLM hardware, analytics probes have been the focus a variety studies are now commonly used in laboratories across world. Protocol reliability artefact identification increasingly seen important aspects super-resolution microscopy. The these approaches thus requires in-depth evaluation so that biological...
Propionic acid (PPA) is used to study the role of mitochondrial dysfunction in neurodevelopmental conditions like autism spectrum disorders. PPA known disrupt biogenesis, metabolism, and turnover. However, effect on dynamics, fission, fusion remains challenging due complex temporal nature these mechanisms. Here, we use complementary quantitative visualization techniques examine how influences ultrastructure, morphology, dynamics neuronal-like SH-SY5Y cells. (5 mM) induced a significant...
Infections in broilers and broiler breeders by Enterococcus cecorum, causing clinical disease, have increasingly been described various countries the Northern Hemisphere over past decade. This case report describes an outbreak of enterococcal-associated vertebral osteoarthritis (EVOA) male several flocks South Africa. Male birds aged 4 9 weeks displayed common presentation lameness, paresis or complete paralysis. Autopsies culled revealed masses on caudal thoracic vertebrae T5-T7, with...
Mammalian cells display a broad spectrum of phenotypes, morphologies, and functional niches within biological systems. Our understanding mechanisms at the individual cellular level, how function in concert to form tissues, organs systems, has been greatly facilitated by centuries' extensive work classify characterize cell types. Classic histological approaches are now complemented with advanced single-cell sequencing spatial transcriptomics for identity studies. Emerging data suggests that...
The first step of cellular entry for the human immunodeficiency virus type-1 (HIV-1) occurs through binding its envelope protein (Env) with plasma membrane receptor CD4 and co-receptor CCR5 or CXCR4 on susceptible cells, primarily CD4+ T cells macrophages. Although there is considerable knowledge molecular interactions between Env host cell receptors that lead to successful fusion, precise way in which HIV-1 redistribute sites at nanoscale remains unknown. Here, we quantitatively examine...
In September 2023, the two largest bioimaging networks in Americas, Latin America Bioimaging (LABI) and BioImaging North (BINA), came together during a 1-week meeting Mexico. This provided opportunities for participants to interact closely with decision-makers from imaging core facilities across Americas. The was held hybrid format attended in-person by scientists including Canada, United States, Mexico, Colombia, Peru, Argentina, Chile, Brazil Uruguay. aims of were discuss progress achieved...
Most super-resolution microscopy methods depend on steps that contribute to the formation of image artefacts. Here we present NanoJ-SQUIRREL, an ImageJ-based analytical approach providing a quantitative assessment quality. By comparing diffraction-limited images and equivalents same focal volume, this generates map defects, as well for their correction. To illustrate its broad applicability approaches apply our method Localization Microscopy, STED SIM variety in-cell structures including...
Single-molecule localization microscopy (SMLM) techniques allow near molecular scale resolution (~ 20nm) as well precise and robust analysis of protein organization at different scales. SMLM hardware, analytics probes have been the focus a variety studies are now commonly used in laboratories across world. Protocol reliability artefact identification increasingly seen important aspects super-resolution microscopy. The these approaches thus requires in-depth evaluation so that biological...
The first step of cellular entry for the human immunodeficiency virus type-1 (HIV-1) occurs through binding its envelope protein (Env) with plasma membrane receptor CD4 and co-receptor CCR5 or CXCR4 on susceptible cells, primarily + T cells macrophages. Although there is considerable knowledge molecular interactions between Env host cell receptors that lead to successful fusion, precise way in which HIV-1 redistribute sites at nanoscale remains unknown. Here, we quantitatively examine...
Mitochondrial dysregulation is implicated in numerous neurological disorders. dynamics, including biogenesis, fusion and fission, are essential components of mitostasis which modulated by complex regulatory mechanisms. Although expression studies often used to investigate mitochondrial these may be limited the interdependent temporal nature mitostasis. Transmission electron microscopy (TEM) cryogenic preparation methods provide a direct approach examine ultrastructure neurons. We...