A5058789705- RNA modifications and cancer
- Immune responses and vaccinations
- Cell Image Analysis Techniques
- RNA Research and Splicing
- Single-cell and spatial transcriptomics
- Cancer-related molecular mechanisms research
- interferon and immune responses
- Tuberculosis Research and Epidemiology
- Genomics and Chromatin Dynamics
- RNA and protein synthesis mechanisms
- Immune cells in cancer
- Cytokine Signaling Pathways and Interactions
- Advanced Fluorescence Microscopy Techniques
- Mycobacterium research and diagnosis
- Immune Response and Inflammation
- CRISPR and Genetic Engineering
- Advanced biosensing and bioanalysis techniques
- Epigenetics and DNA Methylation
- COVID-19 Impact on Reproduction
- Gene Regulatory Network Analysis
- RNA Interference and Gene Delivery
- Immunotherapy and Immune Responses
- Advanced Electron Microscopy Techniques and Applications
- Bioinformatics and Genomic Networks
- Bacterial Genetics and Biotechnology
Radboud University Nijmegen
2019-2025
Radboud University Medical Center
2019-2025
Radboud Institute for Molecular Life Sciences
2019-2025
University Medical Center
2020-2024
Oncode Institute
2024
University of Lisbon
2011-2022
University of Cape Town
2016-2022
Institute of Molecular Medicine
2019-2022
Wellcome Centre for Infectious Diseases Research in Africa
2018-2020
Council for Scientific and Industrial Research
2010-2018
The recent advent of methods for high-throughput single-cell molecular profiling has catalyzed a growing sense in the scientific community that time is ripe to complete 150-year-old effort identify all cell types human body. Human Cell Atlas Project an international collaborative aims define terms distinctive profiles (such as gene expression profiles) and connect this information with classical cellular descriptions location morphology). An open comprehensive reference map state cells...
Nuclear factor κB (NF-κB) essential modulator (NEMO), a regulatory component of the IκB kinase (IKK) complex, controls NF-κB activation through its interaction with ubiquitin chains. We show here that stimulation interleukin-1 (IL-1) and TNF induces rapid transient recruitment NEMO into punctate structures are anchored at cell periphery. These enriched in activated IKK kinases ubiquitinated molecules, which suggests they serve as organizing centers for NF-κB. NEMO-containing colocalize...
We have visualized the movements of native mRNAs in living cells. Using nuclease-resistant molecular beacons, we imaged transport and localization oskar mRNA Drosophila melanogaster oocytes. When pattern was altered by genetic manipulation mRNA's 3′ untranslated region, or chemical perturbation intracellular tubulin network, distribution fluorescence signals changed accordingly. tracked migration real time, from nurse cells where it is produced to posterior cortex oocyte localized. Our...
Background. Statins are cholesterol-lowering drugs, targeting HMG-CoA reductase, thereby reducing the risk of coronary disorders and hypercholesterolemia. However, they also can influence immunologic responses. Methods. Peripheral blood mononuclear cells (PBMCs) monocyte-derived macrophages (MDMs) were isolated from patients with familial hypercholesterolemia (FH) during statin therapy. After infection Mycobacterium tuberculosis, bacterial burden was determined. In vivo, mice treated statins...
The tuberculosis vaccine bacillus Calmette-Guérin (BCG) protects against some heterologous infections, probably via induction of non-specific innate immune memory in monocytes and natural killer (NK) cells, a process known as trained immunity. Recent studies have revealed that the immunity is associated with bias toward granulopoiesis bone marrow hematopoietic progenitor but it unknown whether BCG vaccination also leads to functional reprogramming mature neutrophils. Here, we show healthy...
Using the URL at end of this item, readers can immediately offer feedback and suggestions on topic. Accelerated by human genome project[[HN1][1]], an increasing number genetic variations, many as small a single nucleotide substitution[[HN2][2]], have been found to play
Trained immunity confers a sustained augmented response of innate immune cells to secondary challenge, via process dependent on metabolic and transcriptional reprogramming. Because its previous associations with memory, as well the importance H3 histone lysine 4 monomethylation (H3K4me1) we hypothesize that Set7 methyltransferase has an important role in trained induced by β-glucan. Using pharmacological studies human primary monocytes, identify immunity-specific immunometabolic pathways...
The cause of autosomal-dominant retinitis pigmentosa (adRP), which leads to loss vision and blindness, was investigated in families lacking a molecular diagnosis. A refined locus for adRP on Chr17q22 (RP17) delineated through genotyping genome sequencing, leading the identification structural variants (SVs) that segregate with disease. Eight different complex SVs were characterized 22 adRP-affected >300 affected individuals. All RP17 had breakpoints within genomic region spanning YPEL2...
possesses a large number of genes unknown or predicted function, undermining fundamental understanding pathogenicity and drug susceptibility. To address this challenge, we developed high-throughput functional genomics approach combining inducible CRISPR-interference image-based analyses morphological features sub-cellular chromosomal localizations in the related non-pathogen,
When oligonucleotide probes are microinjected into cells to image the distribution of RNAs, they rapidly sequestered nucleus. As a result, it is difficult detect mRNAs in cytoplasm living cells. We were able overcome this process by attaching tRNA transcripts probes. show that when fluorescently labeled tRNAs, tRNAs with extensions at their 5′ end, or chimeric molecules which molecular beacon possessing 2′- O -methylribonucleotide backbone linked tRNA, injected nucleus HeLa cells, exported...
Abstract The recent advent of methods for high-throughput single-cell molecular profiling has catalyzed a growing sense in the scientific community that time is ripe to complete 150-year-old effort identify all cell types human body, by undertaking Human Cell Atlas Project as an international collaborative effort. aim would be define terms distinctive profiles (e.g., gene expression) and connect this information with classical cellular descriptions location morphology). A comprehensive...
Long noncoding RNAs (lncRNAs) have emerged as critical regulators of gene expression, yet their contribution to immune regulation in humans remains poorly understood. Here, we report that the primate-specific lncRNA CHROMR is induced by influenza A virus and SARS-CoV-2 infection coordinates expression interferon-stimulated genes (ISGs) execute antiviral responses. depletion human macrophages reduces histone acetylation at regulatory regions ISG loci attenuates response microbial stimuli....