A5066746510- Immune responses and vaccinations
- Immune cells in cancer
- Neuroinflammation and Neurodegeneration Mechanisms
- Neurological Disease Mechanisms and Treatments
- Tryptophan and brain disorders
- Immunotherapy and Immune Responses
- Acute Ischemic Stroke Management
- Functional Brain Connectivity Studies
- Single-cell and spatial transcriptomics
- Oral microbiology and periodontitis research
- Immunodeficiency and Autoimmune Disorders
- Angiogenesis and VEGF in Cancer
- Syphilis Diagnosis and Treatment
- Physical Activity and Health
- Immune Response and Inflammation
- Dietary Effects on Health
- Medical Imaging Techniques and Applications
- Adrenal Hormones and Disorders
- Reproductive Physiology in Livestock
- Reproductive tract infections research
- Cerebrospinal fluid and hydrocephalus
- Neurotransmitter Receptor Influence on Behavior
- Diet and metabolism studies
- Advanced Radiotherapy Techniques
- Neonatal Respiratory Health Research
Radboud University Nijmegen
2017-2024
Radboud University Medical Center
2017-2024
Radboud Institute for Molecular Life Sciences
2019-2021
University Medical Center
2020-2021
Amsterdam UMC Location Vrije Universiteit Amsterdam
2015-2017
New York University
1993-2004
Columbia University Irving Medical Center
1993-2004
Trained immunity confers a sustained augmented response of innate immune cells to secondary challenge, via process dependent on metabolic and transcriptional reprogramming. Because its previous associations with memory, as well the importance H3 histone lysine 4 monomethylation (H3K4me1) we hypothesize that Set7 methyltransferase has an important role in trained induced by β-glucan. Using pharmacological studies human primary monocytes, identify immunity-specific immunometabolic pathways...
Rationale: Exposure to high catecholamine levels is associated with inflammatory changes of myeloid cells and atherosclerosis, but the underlying mechanisms are only partly understood. Objective: To investigate whether proinflammatory effects noradrenaline adrenaline can, in part, be explained by induction an immunologic memory innate immune cells, termed trained immunity. Methods Results: In vitro, we exposed human primary monocytes (nor)adrenaline for 24 hours, after which were rested...
Systemic inflammation induced by periodontitis is suggested to be the link between and cardiovascular disease. The aim of this work was explore oral microbiome in relation disease severity systemic inflammation. saliva subgingival from periodontal pocket samples patients with severe (n = 12) mild 13) were analyzed using metagenomic shotgun sequencing. taxa pathways abundances quantified. diversity assessed phenotype associations performed ANCOM linear regression. A panel inflammatory markers...
Background and Purpose— Cerebral small vessel disease (cSVD) is the major vascular cause of cognitive decline dementia. The pathogenesis cSVD remains largely unknown, although several studies suggest a role for systemic inflammation. In certain pathophysiological situations, monocytes can reprogram toward long-term proinflammatory phenotype, which has been termed trained immunity. We hypothesize that immunity contributes to progression cSVD. Methods— Individuals with mild-to-severe...
Atherosclerosis is the major cause of cardiovascular disease (CVD). Monocyte-derived macrophages are most abundant immune cells in atherosclerotic plaques. In patients with CVD, leukocytes have a hyperinflammatory phenotype. We hypothesize that cell reprogramming these occurs at level myeloid progenitors. included 13 coronary artery due to severe atherosclerosis and subjects without an exploratory study. Cytokine production capacity after ex vivo stimulation peripheral blood mononuclear...
Background Low-grade inflammation, largely mediated by monocyte-derived macrophages, contributes to atherosclerosis. Sedentary behavior is associated with atherosclerosis and cardiovascular diseases (CVD). We examined whether reducing sedentary improving walking time improves monocyte inflammatory phenotype in subjects increased risk. Methods Results Across 2 waves, 16 individuals risk performed a 16-week intervention study (age 64±6 years, body mass index 29.9±4.3 kg/m2), using device...
Abstract Context Primary aldosteronism (PA) confers an increased risk of cardiovascular disease (CVD), independent blood pressure. Animal models have shown that aldosterone accelerates atherosclerosis through proinflammatory changes in innate immune cells; human data are scarce. Objective The objective this article is to explore whether patients with PA arterial wall inflammation, systemic and reprogramming monocytes. Design A cross-sectional cohort study compared vascular inflammation on...
Aims Periodontitis is an independent risk factor for cardiovascular disease, but the mechanistic link not fully understood. In atherosclerotic monocytes can adopt a persistent hyperresponsive phenotype, termed trained immunity. We hypothesized that periodontitis-associated bacteria induce immunity in monocytes, which subsequently accelerate atherosclerosis development. Materials and Methods combined vitro experiments on human primary vivo techniques patients with periodontitis to test this...
Chlamydia trachomatis (Ct) is the leading cause of bacterial sexually transmitted diseases (STD) worldwide. The Ct Multi Locus Sequence Typing (MLST) scheme effective in differentiating strain types (ST), deciphering transmission patterns and treatment failure, identifying recombinant strains. Here, we analyzed 323 reference clinical samples, including 58 samples from Russia, an area that has not previously been represented typing schemes, to expand our knowledge global diversification STs....
Intracellular pattern-recognition receptors NOD1 and NOD2 are capable of sensing common structural units bacterial walls. Recognition triggers specific immune signalling pathways leads to pro-inflammatory cytokine upregulation adequate response. We investigated whether two functional polymorphisms in exert an effect on susceptibility (STD patients) severity (female patients visiting the fertility clinic) Chlamydia trachomatis infection 807 Dutch Caucasian women. A significant association...
Neuroimaging in older adults commonly reveals signs of cerebral small vessel disease (SVD). SVD is believed to be caused by chronic hypoperfusion based on animal models and longitudinal studies with inter-scan intervals years. Recent imaging evidence, however, suggests a role for acute ischaemia, as indicated incidental diffusion-weighted lesions (DWI+ lesions), the origin SVD. Furthermore, it becomes increasingly recognised that focal likely affect structure function brain areas remote from...
Background: The etiology of cerebral small vessel disease (SVD) remains elusive, though evidence is accumulating that inflammation contributes to its pathophysiology. We recently showed retrospectively pro-inflammatory monocytes are associated with the long-term progression white matter hyperintensities (WMHs). In this prospective high-frequency imaging study, we hypothesize incidence SVD coincides a monocyte phenotype. Methods: Individuals underwent monthly magnetic resonance (MRI) for 10...
Major surgery such as coronary artery bypass grafting (CABG) is associated with an increased post-operative risk of atherosclerotic cardiovascular events. Cells the innate immune system can adopt a long-lasting pro-inflammatory and atherogenic phenotype after brief exposure to exogenous or endogenous inflammatory stimuli, process called "trained immunity". We hypothesized that surgery-induced inflammation leads sustained alterations in monocyte function, which promote subsequent occurrence...
Trained immunity confers a sustained augmented response of innate immune cells to secondary challenge, via process dependent on metabolic and epigenetic reprogramming. Because its previous associations memory, as well the importance H3 histone lysine 4 monomethylation (H3K4me1) we hypothesized that Set7 methyltransferase has an important role in trained induced by prototypical inducer β-glucan. Using pharmacological studies human primary monocytes, identified immunity-specific...