A5001436573- Research on Leishmaniasis Studies
- Immune responses and vaccinations
- HIV Research and Treatment
- HIV-related health complications and treatments
- Cytokine Signaling Pathways and Interactions
- COVID-19 Impact on Reproduction
- Vaccine Coverage and Hesitancy
- Parasites and Host Interactions
- Fungal Infections and Studies
- Immune Cell Function and Interaction
- Antifungal resistance and susceptibility
- Trypanosoma species research and implications
- HIV/AIDS Research and Interventions
- Leptospirosis research and findings
- Healthcare during COVID-19 Pandemic
- Nail Diseases and Treatments
- Pharmacological Effects of Natural Compounds
- HIV/AIDS drug development and treatment
- Adrenal Hormones and Disorders
- Toxin Mechanisms and Immunotoxins
- Atherosclerosis and Cardiovascular Diseases
- Viral Infections and Vectors
- Cytomegalovirus and herpesvirus research
- Endometriosis Research and Treatment
- Business and Management Studies
Radboud University Nijmegen
2016-2025
Radboud University Medical Center
2016-2025
University Medical Center
2017-2024
Radboud Institute for Molecular Life Sciences
2019-2023
Universidade Federal de Goiás
2016-2020
Instituto Dante Pazzanese de Cardiologia
2018
Pontifícia Universidade Católica de Goiás
2014
American tegumentary leishmaniasis is a vector-borne parasitic disease caused by Leishmania protozoans. Innate immune cells undergo long-term functional reprogramming in response to infection or Bacillus Calmette-Guérin (BCG) vaccination via process called trained immunity, conferring non-specific protection from secondary infections. Here, we demonstrate that monocytes with the fungal cell wall component β-glucan confer enhanced against infections braziliensis through production of...
Trained immunity confers a sustained augmented response of innate immune cells to secondary challenge, via process dependent on metabolic and transcriptional reprogramming. Because its previous associations with memory, as well the importance H3 histone lysine 4 monomethylation (H3K4me1) we hypothesize that Set7 methyltransferase has an important role in trained induced by β-glucan. Using pharmacological studies human primary monocytes, identify immunity-specific immunometabolic pathways...
A growing number of studies show that innate immune cells can undergo functional reprogramming, facilitating a faster and enhanced response to heterologous secondary stimuli. This concept has been termed "trained immunity." We outline here protocol recapitulate this in vitro using adherent monocytes from consecutive isolation peripheral blood mononuclear cells. The induction trained immunity the associated reprogramming is described detail β-glucan (from Candida albicans) Bacillus...
Innate immune cells can undergo long-term functional reprogramming after certain infections, a process called trained immunity (TI). Here, we focus on antigens of Leishmania braziliensis, which induced anti-tumor effects via in human monocytes. We reveal that monocytes exposed to promastigote L. braziliensis develop an enhanced response subsequent exposure Toll-like receptor (TLR)2 or TLR4 ligands. Mechanistically, the induction TI by is mediated multiple pattern recognition receptors,...
Background Interleukin-32 (IL-32) is expressed in lesions of patients with American Tegumentary Leishmaniasis (ATL), but its precise role the disease remains unknown. Methodology/Principal findings In present study, silencing and overexpression IL-32 was performed THP-1-derived macrophages infected Leishmania (Viannia) braziliensis or L. (Leishmania) amazonensis to investigate infection. We report that species induces IL-32γ, show intracellular IL-32γ protein production dependent on...
Due to the increased burden of non-AIDS-related comorbidities in people living with HIV (PLHIV), identifying biomarkers and mechanisms underlying premature aging risk developing age-related is a priority. Evidence suggests that plasma proteome an accurate source for measuring biological age predicting clinical outcomes. To investigate whether PLHIV on antiretroviral therapy (ART) exhibit phenotype, we profiled two independent cohorts virally suppressed (200HIV 2000HIV) one cohort without...
Background: FSHD is a highly prevalent inherited myopathy with still poorly understood pathology. Objective: To investigate whether proinflammatory cytokines are associated and which specific innate immune cells involved in its Methods: First, we measured circulating serum samples: IL-6 (FSHD, n = 150; HC, 98); TNF 59); IL-1α 66); IL-1β MCP-1 14; 14); VEGF-A 14). Second, tested trained immunity monocytes 15; 15) NK 11; 11). Next, explored the cytokine production capacity of response to...
Background Even during long-term combination antiretroviral therapy (cART), people living with HIV (PLHIV) have a dysregulated immune system, characterized by persistent activation, accelerated ageing and increased risk of non-AIDS comorbidities. A multi-omics approach is applied to large cohort PLHIV understand pathways underlying these dysregulations in order identify new biomarkers novel genetically validated therapeutic drugs targets. Methods The 2000HIV study prospective longitudinal on...
Introduction People living with HIV (PLHIV) are characterized by functional reprogramming of innate immune cells even after long-term antiretroviral therapy (ART). In order to assess technical feasibility omics technologies for application larger cohorts, we compared multiple data layers. Methods Bulk and single-cell transcriptomics, flow cytometry, proteomics, chromatin landscape analysis ATAC-seq as well ex vivo drug stimulation were performed in a small number blood samples derived from...
Abstract Studies in IL-32 transgenic mice and vitro suggest that may have protective effects against Mycobacterium tuberculosis, but so far there are barely any studies humans. We studied the role of its splice variants tuberculosis (TB) vivo vitro. Blood transcriptional analysis showed lower total mRNA levels pulmonary TB patients compared to with latent infection healthy controls. Also, among Indonesian household contacts who were heavily exposed an infectious patient, higher those...
Sporotrichosis is a deep mycosis caused by dimorphic species of the genus Sporothrix, with differences in pathogenicity between S. schenckii and brasiliensis species. Recently, it was discovered that cell wall peptidorhamnomannan (PRM) from has additional unknown rhamnose residues. We hypothesize structural Sporothrix spp PRMs impact host's immune response may explain severity sporotrichosis brasiliensis. demonstrate yeasts its PRM (S.b PRM) induced strong inflammatory human PBMCs, high...
Abstract Patients with rheumatoid arthritis (RA) are at higher risk of developing cardiovascular diseases (CVD). Interleukin (IL)-32 has previously been shown to be involved in the pathogenesis RA and might linked development atherosclerosis. However, exact mechanism linking IL-32 CVD still needs elucidated. The influence a functional genetic variant on lipid profiles was therefore studied whole blood from individuals NBS cohort patients 2 independent cohorts. Lipid were matched specific...
Interleukin 32 (IL-32) is a pro-inflammatory cytokine induced in patients with American tegumentary leishmaniasis (ATL) caused by Leishmania braziliensis. Here, we investigated whether IL-32 also expressed patient lesions L. amazonensis. In addition, evaluated experimental amazonensis and braziliensis infections C57BL/6 transgenic mice for human IL-32γ (IL-32γTg) comparison wild-type (WT) that do not express the gene. Human cutaneous higher levels of than healthy control skin. mice, presence...
American Tegumentary Leishmaniasis is a chronic infection caused by Leishmania protozoan. It not known whether genetic variances in NOD-like receptor (NLR) family members influence the immune response towards parasites and modulate intracellular killing. Using functional genomics, we investigated variants NOD1 or NOD2 production of cytokines human PBMCs exposed to Leishmania. In addition, examined recognition contributes Polymorphisms gene decreased monocyte- lymphocyte-derived cytokine...
The role of interleukin (IL-)32 in inflammatory conditions is well-established, however, the mechanism behind its atherosclerosis remains unexplained. Our group reported a promoter single nucleotide polymorphism IL-32 associated with higher high-density lipoprotein (HDL) concentrations. We hypothesize that endogenous liver cells, human monocytic cell line and carotid plaque tissue, can affect by regulating cholesterol homeostasis via expression transporters/mediators.Human primary cells were...