A5060776627- HIV Research and Treatment
- HIV-related health complications and treatments
- Liver Disease Diagnosis and Treatment
- Diet and metabolism studies
- Gut microbiota and health
- Immune Cell Function and Interaction
- Fibroblast Growth Factor Research
- Diabetes and associated disorders
- Tryptophan and brain disorders
- Epigenetics and DNA Methylation
- Blood Coagulation and Thrombosis Mechanisms
- Adipokines, Inflammation, and Metabolic Diseases
- Metabolomics and Mass Spectrometry Studies
- Fatty Acid Research and Health
- HIV/AIDS drug development and treatment
- Cytomegalovirus and herpesvirus research
- COVID-19 epidemiological studies
- Diet, Metabolism, and Disease
- T-cell and B-cell Immunology
- Clostridium difficile and Clostridium perfringens research
- Advanced Proteomics Techniques and Applications
- Birth, Development, and Health
- Vaccine Coverage and Hesitancy
- Viral Infections and Vectors
- Hemoglobinopathies and Related Disorders
Radboud University Nijmegen
2021-2025
Radboud University Medical Center
2021-2025
University Medical Center
2023-2024
Dr. Kariadi Hospital
2021-2023
Diponegoro University
2021-2023
Abstract Background Steatotic liver disease is suggested to have a higher prevalence and severity in people with HIV (PHIV), including those normal body mass index (BMI). In this study, we used data from the 2000HIV cohort (1) assess of steatosis fibrosis lean versus overweight/obese PHIV (2) associations these subgroups between traditional risk factors HIV-specific characteristics. Methods The study comprises 1895 virally suppressed that were included 2019 2021 4 treatment centers...
Metabolic dysfunction-associated steatotic liver disease (MASLD) is a key risk factor for cardiovascular (CVD), potentially driven by shared metabolic mechanisms. perturbations associated with MASLD and CVD remain underexplored in people HIV (PWH). We used data from the longitudinal multicenter 2000HIV study comprising 1895 virally suppressed PWH, out of which 970 had available carotid artery measurements. Transient elastography controlled attenuation parameter (CAP) was performed assessment...
People living with human immunodeficiency virus (PLHIV) are exposed to chronic immune dysregulation, even when replication is suppressed by antiretroviral therapy (ART). Given the emerging role of gut microbiome in immunity, we hypothesized that may be related cytokine production capacity PLHIV.
Immunological non-responders (INR) are people living with HIV (PLHIV) who fail to fully restore CD4+ T-cell counts despite complete viral suppression antiretroviral therapy (ART). INR at higher risk for non-HIV related morbidity and mortality. Previous research suggest persistent qualitative defects. The 2000HIV study (clinical trials NTC03994835) enrolled 1895 PLHIV, divided in a discovery validation cohort. PLHIV CD4 count <350 cells/mm3 after ≥2 years of suppressive ART were defined as...
Background Even during long-term combination antiretroviral therapy (cART), people living with HIV (PLHIV) have a dysregulated immune system, characterized by persistent activation, accelerated ageing and increased risk of non-AIDS comorbidities. A multi-omics approach is applied to large cohort PLHIV understand pathways underlying these dysregulations in order identify new biomarkers novel genetically validated therapeutic drugs targets. Methods The 2000HIV study prospective longitudinal on...
Background Cardiovascular disease is a major cause of morbidity and mortality in people living with HIV, who are at higher risk than the general population. We assessed, large cohort which cardiovascular, HIV-specific, lipoproteomic markers were associated carotid intima-media thickness (cIMT) plaque presence. also studied guideline adherence on lipid-lowering medication individuals high very for cardiovascular disease. Methods Results In 1814 median (interquartile range) age 53 (44-60)...
BackgroundGeographic variability in coagulation across populations and their determinants are poorly understood.ObjectiveTo compare thrombin (TG) plasmin (PG) generation parameters between healthy Tanzanian Dutch individuals, to study associations with inflammation different genetic, host environmental factors.MethodsTG PG were measured 313 Tanzanians of African descent living Tanzania 392 European the Netherlands related results a dietary questionnaire, circulating inflammatory markers,...
In people living with HIV (PLHIV), integrase strand transfer inhibitors (INSTIs) are part of the first-line combination antiretroviral therapy (cART), while non-nucleoside reverse transcriptase inhibitor (NNRTI)-based regimens alternatives. Distinct cART may variably influence risk for non-AIDS comorbidities. We aimed to compare metabolome and lipidome INSTI NNRTI-based regimens. The 2000HIV study includes asymptomatic PLHIV (n = 1646) on long-term cART, separated into a discovery cohort 730...
We aimed to compare the non-AIDS events (nADE) risk between normal progressors using ART (NP-ART) and people with HIV (PWH) that naturally control infection (HIV controllers), as well outcomes after in controllers on nADE.
Background The immunopathogenesis of dengue virus (DENV) infection remains incompletely understood. To increase our understanding inflammatory response in non-severe dengue, we assessed longitudinal changes the proteome patients with an acute DENV infection. Methods Using a multiplex proximity extension assay (PEA), measured relative levels 368 markers plasma samples from hospitalized (n = 43) and convalescence 35) phase healthy controls 10). Results We identified 203 upregulated 39...
BACKGROUNDPeople living with HIV (PLHIV) receiving antiretroviral therapy (ART) exhibit persistent immune dysregulation and microbial dysbiosis, leading to development of cardiovascular diseases (CVDs). We initially compared plasma proteomic profiles between 205 PLHIV 120 healthy control participants (HCs) validated the results in an independent cohort 639 99 HCs. Differentially expressed proteins (DEPs) were then associated microbiome data. Finally, we assessed which linked CVD...
Non-communicable diseases (NCDs) are rising rapidly in urbanizing populations sub-Saharan Africa. Assessment of inflammatory and metabolic characteristics a African population the comparison with outside Africa could provide insight pathophysiology increasing epidemic NCDs, including role environmental dietary changes. Using proteomic plasma profiling approach comprising 92 inflammation-related molecules, we examined differences proteome healthy Tanzanian Dutch adults. We show that...
People living with HIV (PLHIV) exhibit dysregulation of tryptophan metabolism. Altered gut microbiome composition in PLHIV might be involved. Mechanistic consequences within the 3 major metabolism pathways (serotonin, kynurenine, and indoles), functional for platelet, immune behavioral functions are unknown. We investigated plasma metabolites, composition, their association platelet function, inflammation, psychiatric symptoms.
Psychiatric symptoms are prevalent in people living with HIV (PLWH), especially depression, anxiety, impulsivity, and substance use. Various biological mechanisms might play a role the occurrence of psychiatric this population. A hypothesis free, data-driven metabolomics approach can further our understanding these mechanisms. In study, we identified metabolic pathways associated depression use 157 PLWH. First, Spearman's rank correlations between metabolite feature intensities symptom...
Genetics play a significant role in coagulation phenotype and venous thromboembolism risk. Resistance to the anticoagulant activated protein C (APC) is an established risk for thrombosis. Herein, we explored genetic determinants of thrombin generation (TG) thrombomodulin (TM)-modulated TG using plasma from Human Functional Genomics Project.Calibrated was measured both absence presence TM tissue factor as trigger. Genetic parameters pathway function were assessed genome-wide single-nucleotide...
To bridge the gap between genetics and disease biology, it is crucial to explore relationship genetic variations plasma inflammatory protein concentrations. Studies in European cohorts have shown a significant impact of variation on regulation proteins. African populations are underrepresented immunogenetic studies. We aimed compare 72 proteins using two Human Functional Genomics Project (http://www.humanfunctionalgenomics.org): cohort 286 healthy East individuals from Tanzania 340 Western...
During the COVID-19 pandemic, major events with immune-modulating effects at population-level included infection, lockdowns, and mass vaccinations campaigns. As immune responses influence many immune-mediated diseases, population scale immunological changes may have broad consequences.
HIV-1 reservoir shows high variability in size and activity among virally suppressed individuals. Differences the of viral may relate to differences plasma protein concentrations. We tested whether expression levels are associated with cell-associated (CA) DNA RNA 211 people living HIV (PLHIV). Plasma concentrations FOLR1, IL1R1, MICA, FETUB showed a positive association CA DNA. Moreover, SNPs close proximity IL1R1 MICA genes were found influence difference mRNA gene homozygotes carrying...
Platelets play a key role in hemostasis, inflammation, and cardiovascular diseases. Platelet reactivity is highly variable between individuals. The drivers of this variability populations from Sub-Saharan Africa remain largely unknown.
Abstract People living with HIV (PLHIV) are exposed to chronic immune dysregulation, even when virus replication is suppressed by antiretroviral therapy (ART). Given the emerging role of gut microbiome in immunity, we hypothesized that may be related cytokine production capacity PLHIV. To test this hypothesis, collected metagenomic data from 143 ART-treated PLHIV and assessed ex vivo eight different cytokines (IL-1β, IL-6, IL-1Ra, IL-10, IL17, IL22, TNF IFN-γ) response stimuli. We also...