Rob J.W. Arts
A5066547819- Immune responses and vaccinations
- Immune cells in cancer
- COVID-19 Impact on Reproduction
- Vaccine Coverage and Hesitancy
- Diabetes and associated disorders
- Immunodeficiency and Autoimmune Disorders
- Immune Response and Inflammation
- Epigenetics and DNA Methylation
- Neonatal Respiratory Health Research
- Inflammation biomarkers and pathways
- Immune Cell Function and Interaction
- Tuberculosis Research and Epidemiology
- Ubiquitin and proteasome pathways
- Inflammasome and immune disorders
- Genetic Neurodegenerative Diseases
- Plant biochemistry and biosynthesis
- Muscle Physiology and Disorders
- COVID-19 Clinical Research Studies
- Extracellular vesicles in disease
- NF-κB Signaling Pathways
- Heat shock proteins research
- Neuroinflammation and Neurodegeneration Mechanisms
- Child Nutrition and Water Access
- Histiocytic Disorders and Treatments
- Cardiomyopathy and Myosin Studies
Radboud University Nijmegen
2015-2024
Radboud University Medical Center
2015-2024
University Medical Center
2014-2023
Radboud Institute for Molecular Life Sciences
2019-2023
University of Colorado Denver
2021
Epigenetic reprogramming of myeloid cells, also known as trained immunity, confers nonspecific protection from secondary infections. Using histone modification profiles human monocytes with the Candida albicans cell wall constituent β-glucan, together a genome-wide transcriptome, we identified induced expression genes involved in glucose metabolism. Trained display high consumption, lactate production, and ratio nicotinamide adenine dinucleotide (NAD(+)) to its reduced form (NADH),...
The protective effects of the tuberculosis vaccine Bacillus Calmette-Guerin (BCG) on unrelated infections are thought to be mediated by long-term metabolic changes and chromatin remodeling through histone modifications in innate immune cells such as monocytes, a process termed trained immunity. Here, we show that BCG induction immunity monocytes is accompanied strong increase glycolysis and, lesser extent, glutamine metabolism, both an in-vitro model after vaccination mice humans....
Innate immune memory is the phenomenon whereby innate cells such as monocytes or macrophages undergo functional reprogramming after exposure to microbial components lipopolysaccharide (LPS). We apply an integrated epigenomic approach characterize molecular events involved in LPS-induced tolerance a time-dependent manner. Mechanistically, LPS-treated fail accumulate active histone marks at promoter and enhancers of genes lipid metabolism phagocytic pathways. Transcriptional inactivity...
Recent evidence suggests that certain vaccines, including Bacillus-Calmette Guérin (BCG), can induce changes in the innate immune system with non-specific memory characteristics, termed 'trained immunity'. Here we present results of a randomised, controlled phase 1 clinical trial 20 healthy male and female volunteers to evaluate induction immunity protective efficacy anti-tuberculosis BCG vaccine against human malaria infection. After challenge infection, vaccinated earlier more severe...
The lack of defined correlates protection hampers development vaccines against tuberculosis (TB). In vitro mycobacterial outgrowth assays are thought to better capture the complexity human host/Mycobacterium (Mtb) interaction. Here, we used a growth inhibition assay (MGIA) based on peripheral blood mononuclear cells investigate capacity control bacille Calmette-Guérin (BCG). Interestingly, strong BCG was observed almost exclusively in individuals with recent exposure Mtb, but not (long-term)...
BACKGROUNDInduction of innate immune memory, also termed trained immunity, by the antituberculosis vaccine bacillus Calmette-Guérin (BCG) contributes to protection against heterologous infections. However, overall impact BCG vaccination on inflammatory status an individual is not known; while induction immunity may suggest increased inflammation, has been epidemiologically associated with a reduced incidence and allergic diseases.METHODSWe investigated (BCG-Bulgaria, InterVax) systemic...
BACKGROUND. The antituberculosis vaccine bacillus Calmette-Guérin (BCG) reduces overall infant mortality. Induction of innate immune memory, also termed trained immunity, contributes toward protection against heterologous infections. Since cells display oscillations in numbers and function throughout the day, we investigated effect BCG administration time on induction immunity.
Tumor-associated macrophages (TAMs) are key components of the tumor microenvironment in non-medullary thyroid cancer (TC), most common endocrine malignancy. However, little is known regarding regulation their function TC. Transcriptome analysis a model TC-induced identified increased inflammatory characteristics and rewiring cell metabolism as functional changes. This reprogramming was partly mediated by TC-derived lactate that induced upregulation cytokine production through an...
Metformin, the most widely administered diabetes drug, has been proposed as a candidate adjunctive host-directed therapy for tuberculosis, but little is known about its effects on human host responses to Mycobacterium tuberculosis.We investigated in vitro and vivo of metformin humans.Metformin added peripheral blood mononuclear cells from healthy volunteers enhanced cellular metabolism while inhibiting mammalian target rapamycin targets p70S6K 4EBP1, with decreased cytokine production...
A growing number of studies show that innate immune cells can undergo functional reprogramming, facilitating a faster and enhanced response to heterologous secondary stimuli. This concept has been termed "trained immunity." We outline here protocol recapitulate this in vitro using adherent monocytes from consecutive isolation peripheral blood mononuclear cells. The induction trained immunity the associated reprogramming is described detail β-glucan (from Candida albicans) Bacillus...
Abstract BCG vaccination is associated with a reduced mortality from nonmycobacterial infections. This likely to be mediated by combination of innate-immune memory (“trained immunity”) and heterologous effects on adaptive immunity. As such, could used boost host immunity but not in immunocompromised hosts, as it live, attenuated vaccine. Therefore, we assessed whether killed γBCG has similar potentiating effects. In an vitro model trained immunity, human monocytes were incubated for 24 h...