Martijn C. Nawijn
A5024463990- Asthma and respiratory diseases
- IL-33, ST2, and ILC Pathways
- Allergic Rhinitis and Sensitization
- Single-cell and spatial transcriptomics
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Neonatal Respiratory Health Research
- Pediatric health and respiratory diseases
- Immune Cell Function and Interaction
- Cancer Mechanisms and Therapy
- Eosinophilic Esophagitis
- Epigenetics and DNA Methylation
- Food Allergy and Anaphylaxis Research
- Wnt/β-catenin signaling in development and cancer
- Dermatology and Skin Diseases
- Respiratory and Cough-Related Research
- Peptidase Inhibition and Analysis
- SARS-CoV-2 and COVID-19 Research
- Mast cells and histamine
- Respiratory viral infections research
- Inhalation and Respiratory Drug Delivery
- COVID-19 Clinical Research Studies
- T-cell and B-cell Immunology
- Cancer-related gene regulation
- Immune Response and Inflammation
- Cancer-related molecular mechanisms research
University Medical Center Groningen
2016-2025
University of Groningen
2016-2025
Institute for Asthma and Allergy
2010-2024
Dialyse Centrum Groningen
2020-2024
Duke University
2021
University College London
2021
Wellcome Sanger Institute
2020
Odense University Hospital
2017
The Netherlands Cancer Institute
2004-2009
Oncode Institute
2004-2008
There is pressing urgency to understand the pathogenesis of severe acute respiratory syndrome coronavirus clade 2 (SARS-CoV-2), which causes disease COVID-19. SARS-CoV-2 spike (S) protein binds angiotensin-converting enzyme (ACE2), and in concert with host proteases, principally transmembrane serine protease (TMPRSS2), promotes cellular entry. The cell subsets targeted by tissues factors that regulate ACE2 expression remain unknown. Here, we leverage human, non-human primate, mouse...
The recent advent of methods for high-throughput single-cell molecular profiling has catalyzed a growing sense in the scientific community that time is ripe to complete 150-year-old effort identify all cell types human body. Human Cell Atlas Project an international collaborative aims define terms distinctive profiles (such as gene expression profiles) and connect this information with classical cellular descriptions location morphology). An open comprehensive reference map state cells...
Single-cell technologies have transformed our understanding of human tissues. Yet, studies typically capture only a limited number donors and disagree on cell type definitions. Integrating many single-cell datasets can address these limitations individual the variability present in population. Here we integrated Human Lung Cell Atlas (HLCA), combining 49 respiratory system into single atlas spanning over 2.4 million cells from 486 individuals. The HLCA presents consensus re-annotation with...
Cellular diversity of the lung endothelium has not been systematically characterized in humans. We provide a reference atlas human endothelial cells (ECs) to facilitate better understanding phenotypic and composition comprising endothelium.We reprocessed control single-cell RNA sequencing (scRNAseq) data from 6 datasets. EC populations were through iterative clustering with subsequent differential expression analysis. Marker genes validated by fluorescent microscopy situ hybridization....
In recent years, functional genomics approaches combining genetic information with bulk RNA-sequencing data have identified the downstream expression effects of disease-associated risk factors through so-called quantitative trait locus (eQTL) analysis. Single-cell creates enormous opportunities for mapping eQTLs across different cell types and in dynamic processes, many which are obscured when using methods. Rapid increase throughput reduction cost per now allow this technology to be applied...
The EMBL-EBI Expression Atlas is an added value knowledge base that enables researchers to answer the question of where (tissue, organism part, developmental stage, cell type) and under which conditions (disease, treatment, gender, etc) a gene or protein interest expressed. brings together data from >4500 expression studies >65 different species, across tissues. It makes these freely available in easy visualise form, after expert curation accurately represent intended experimental design,...
Mast cells in type 2 airway inflammation exhibit heterogeneous inflammation-associated phenotypes and proliferate situ.
ABSTRACT Organ- and body-scale cell atlases have the potential to transform our understanding of human biology. To capture variability present in population, these must include diverse demographics such as age ethnicity from both healthy diseased individuals. The growth size number single-cell datasets, combined with recent advances computational techniques, for first time makes it possible generate comprehensive large-scale through integration multiple datasets. Here, we integrated Human...
The Pim family of proto-oncogenes encodes a distinct class serine/threonine kinases consisting PIM1, PIM2, and PIM3. Although the genes are evolutionarily highly conserved, contribution PIM proteins to mammalian development is unclear. PIM1-deficient mice were previously described but showed only minor phenotypic aberrations. To assess role in physiology, compound knockout generated. Mice lacking expression Pim1, Pim2, Pim3 viable fertile. However, PIM-deficient show profound reduction body...
GATA-3 is a zinc-finger transcription factor that essential for both early T cell development and Th2 differentiation. To quantify expression during in vivo the mouse, gene was targeted by insertion of lacZ reporter homologous recombination embryonic stem (ES) cells. Although we could detect GATA-3+ cells throughout thymus, proportions varied considerably between distinct differentiation stages. The two periods TCR α β recombination, which occur quiescent or slowly dividing cells, were...
Studies of SOCS-1-deficient mice have implicated Socs-1 in the suppression JAK-STAT (Janus tyrosine kinase-signal transducers and activators transcription) signaling T cell development. It has been suggested that levels protein may be regulated through proteasome pathway. Here we show interacts with members Pim family serine/threonine kinases thymocytes. Coexpression results phosphorylation stabilization protein. The are significantly reduced Pim-1(-/-), Pim-2(-/-) as compared wild-type...
Recent data indicate a role for airway epithelial necroptosis, regulated form of necrosis, and the associated release damage-associated molecular patterns (DAMPs) in development chronic obstructive pulmonary disease (COPD). DAMPs can activate pattern recognition receptors (PRRs), triggering innate immune responses. We hypothesized that cigarette smoke (CS)-induced necroptosis DAMP initiate inflammation COPD. Human bronchial BEAS-2B cells were exposed to extract (CSE), necrotic cell death...