Aaron G. Schmidt
A5041178880- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- Monoclonal and Polyclonal Antibodies Research
- Influenza Virus Research Studies
- Immunotherapy and Immune Responses
- SARS-CoV-2 detection and testing
- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Animal Virus Infections Studies
- vaccines and immunoinformatics approaches
- Respiratory viral infections research
- Viral gastroenteritis research and epidemiology
- Mosquito-borne diseases and control
- COVID-19 epidemiological studies
- HIV Research and Treatment
- Virus-based gene therapy research
- CAR-T cell therapy research
- Viral Infections and Immunology Research
- Viral Infections and Outbreaks Research
- Transgenic Plants and Applications
- Immune responses and vaccinations
- Viral Infections and Vectors
- COVID-19 Impact on Reproduction
- interferon and immune responses
- American Environmental and Regional History
Ragon Institute of MGH, MIT and Harvard
2019-2025
Massachusetts General Hospital
2007-2025
Harvard University
2016-2025
TU Dresden
2022-2023
Concord Consortium
2020-2021
Boston VA Research Institute
2020
Boston Children's Hospital
2013-2019
Massachusetts Institute of Technology
2019
Moscow Research and Clinical Center for Neuropsychiatry
2016
University of Iowa Hospitals and Clinics
2016
There is pressing urgency to understand the pathogenesis of severe acute respiratory syndrome coronavirus clade 2 (SARS-CoV-2), which causes disease COVID-19. SARS-CoV-2 spike (S) protein binds angiotensin-converting enzyme (ACE2), and in concert with host proteases, principally transmembrane serine protease (TMPRSS2), promotes cellular entry. The cell subsets targeted by tissues factors that regulate ACE2 expression remain unknown. Here, we leverage human, non-human primate, mouse...
The global coronavirus disease 2019 (COVID-19) pandemic caused by severe acute respiratory syndrome 2 (SARS-CoV-2) has made the development of a vaccine top biomedical priority. In this study, we developed series DNA candidates expressing different forms SARS-CoV-2 spike (S) protein and evaluated them in 35 rhesus macaques. Vaccinated animals humoral cellular immune responses, including neutralizing antibody titers at levels comparable to those found convalescent humans macaques infected...
An understanding of protective immunity to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is critical for vaccine and public health strategies aimed at ending the global disease 2019 (COVID-19) pandemic. A key unanswered question whether infection with SARS-CoV-2 results in against reexposure. We developed a rhesus macaque model observed that macaques had high viral loads upper lower tract, humoral cellular immune responses, pathologic evidence pneumonia. After initial...
We measured plasma and/or serum antibody responses to the receptor-binding domain (RBD) of spike (S) protein SARS-CoV-2 in 343 North American patients infected with (of which 93% required hospitalization) up 122 days after symptom onset and compared them 1548 individuals whose blood samples were obtained prior pandemic. After setting seropositivity thresholds for perfect specificity (100%), we estimated sensitivities 95% IgG, 90% IgA, 81% IgM detecting between 15 28 onset. While median time...
Biological data are lacking with respect to risk of vertical transmission and mechanisms fetoplacental protection in maternal severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection.To quantify SARS-CoV-2 viral load neonatal biofluids, transplacental passage anti-SARS-CoV-2 antibody, incidence infection.This cohort study was conducted among pregnant women presenting for care at 3 tertiary centers Boston, Massachusetts. Women reverse transcription-polymerase chain reaction...
As SARS-CoV-2 infections and death counts continue to rise, it remains unclear why some individuals recover from infection, whereas others rapidly progress die. Although the immunological mechanisms that underlie different clinical trajectories remain poorly defined, pathogen-specific antibodies often point of protection. Here, we profiled SARS-CoV-2-specific humoral responses in a cohort 22 hospitalized individuals. Despite inter-individual heterogeneity, distinct antibody signatures...
Pregnant women are at increased risk of morbidity and mortality from COVID-19 but have been excluded the phase 3 vaccine trials. Data on safety immunogenicity in these populations therefore limited.
Abstract Coronavirus disease 2019 (COVID-19) in humans is often a clinically mild illness, but some individuals develop severe pneumonia, respiratory failure and death 1–4 . Studies of acute syndrome coronavirus 2 (SARS-CoV-2) infection hamsters 5–7 nonhuman primates 8–10 have generally reported clinical disease, preclinical SARS-CoV-2 vaccine studies demonstrated reduction viral replication the upper lower tracts 11–13 Here we show that high-dose intranasal results including high levels...
Affinity maturation refines a naive B-cell response by selecting mutations in antibody variable domains that enhance antigen binding. We describe lineage expressing broadly neutralizing influenza virus antibodies derived from subject immunized with the 2007 trivalent vaccine. The comprises three mature antibodies, unmutated common ancestor, and intermediate. Their heavy-chain complementarity determining region inserts into conserved receptor-binding pocket of HA. show analysis structures,...
As the COVID-19 pandemic continues to spread, investigating processes underlying interactions between SARS-CoV-2 and its hosts is of high importance. Here, we report identification CD209L/L-SIGN related protein CD209/DC-SIGN as receptors capable mediating entry into human cells. Immunofluorescence staining tissues revealed prominent expression CD209L in lung kidney epithelia endothelia. Multiple biochemical assays using a purified recombinant spike receptor-binding domain (S-RBD) or S1...
Abstract We show that SARS-CoV-2 spike protein interacts with cell surface heparan sulfate and angiotensin converting enzyme 2 (ACE2) through its Receptor Binding Domain. Docking studies suggest a putative heparin/heparan sulfate-binding site adjacent to the domain binds ACE2. In vitro, binding of ACE2 heparin ectodomains occurs independently ternary complex can be generated using as template. Contrary purified components, on cells codependently. Unfractionated heparin, non-anticoagulant...
Characterizing the humoral immune response to SARS-CoV-2 and developing accurate serologic assays are needed for diagnostic purposes estimating population-level seroprevalence. We measured kinetics of early antibody responses receptor-binding domain (RBD) spike (S) protein in a cohort 259 symptomatic North American patients infected with (up 75 days after symptom onset) compared levels 1548 individuals whose blood samples were obtained prior pandemic. Between 14-28 from onset symptoms, IgG,...