A5044474928- Proteoglycans and glycosaminoglycans research
- Glycosylation and Glycoproteins Research
- Lysosomal Storage Disorders Research
- Nuclear Structure and Function
- Carbohydrate Chemistry and Synthesis
- RNA Research and Splicing
- Brain Metastases and Treatment
- Glioma Diagnosis and Treatment
- Skin and Cellular Biology Research
- Muscle Physiology and Disorders
- Influenza Virus Research Studies
- COVID-19 Clinical Research Studies
- Viral Infectious Diseases and Gene Expression in Insects
- Meningioma and schwannoma management
- SARS-CoV-2 and COVID-19 Research
- Platelet Disorders and Treatments
- Genomics and Chromatin Dynamics
- Cell Adhesion Molecules Research
- Monoclonal and Polyclonal Antibodies Research
- Cancer, Hypoxia, and Metabolism
- Cellular Mechanics and Interactions
- Meta-analysis and systematic reviews
- Ion channel regulation and function
- Cardiomyopathy and Myosin Studies
- Statistical Methods in Clinical Trials
General Atomics (United States)
2020-2024
Louisiana State University Agricultural Center
2022
Brown University
2014
University of California, San Diego
1992-2013
Thomas Jefferson University
2009-2013
Sidney Kimmel Cancer Center
2013
Neurological Surgery
2010
Johns Hopkins University
2010
Jefferson College
2010
Sidney Kimmel Comprehensive Cancer Center
2010
In response to oxidative stress, the nuclear factor E2-related 2 (Nrf2) transcription translocates from cytoplasm into nucleus and transactivates expression of genes with antioxidant activity. Despite this cellular mechanism, damage is abundant in Alzheimer Parkinson disease (AD PD). To investigate mechanisms by which Nrf2 activity may be aberrant or insufficient neurodegenerative conditions, we assessed localization affected brain regions AD, Lewy body variant AD (LBVAD), PD. By...
Salvage options for recurrent high-grade gliomas (HGGs) are limited by cumulative toxicity and efficacy despite advances in chemotherapeutic radiotherapeutic techniques. Previous studies have reported encouraging survival results favorable with fractionated stereotactic radiotherapy, small shown similar benefit using a shortened course of hypofractionated radiation therapy (H-SRT). We sought to determine the profile H-SRT alone or addition repeat craniotomy concomitant chemotherapy.Between...
Abstract We show that SARS-CoV-2 spike protein interacts with cell surface heparan sulfate and angiotensin converting enzyme 2 (ACE2) through its Receptor Binding Domain. Docking studies suggest a putative heparin/heparan sulfate-binding site adjacent to the domain binds ACE2. In vitro, binding of ACE2 heparin ectodomains occurs independently ternary complex can be generated using as template. Contrary purified components, on cells codependently. Unfractionated heparin, non-anticoagulant...
Interaction of chromatin with the nuclear envelope and lamina is thought to help determine higher order chromosome organization in interphase nucleus. Previous studies have shown that lamins bind directly. Here we localized a binding site carboxyl-terminal tail domains both A- B-type mammalian lamins, characterized biochemical properties this detail. Recombinant glutathione-S-transferase fusion proteins containing C, B1, B2 were analyzed for their ability associate rat liver fragments...
While homomers containing 5-HT3A subunits form functional ligand-gated serotonin (5-HT) receptors in heterologous expression systems (Jackson, M. B., and Yakel, J. L. (1995) Annu. Rev. Physiol. 57, 447–468; Lambert, J., Peters, A., Hope, A. G. Ligand-Voltage-Gated Ion Channels (North, R., ed) pp. 177–211, CRC Press, Inc., Boca Raton, FL), it has been proposed that native may exist as heteromers (Fletcher, S., Barnes, N. (1998) Trends Pharmacol. Sci. 19, 212–215). We report the cloning of a...
In a search for small molecule antagonists of heparan sulfate, we examined the activity bis -2-methyl-4-amino-quinolyl-6-carbamide, also known as surfen. Fluorescence-based titrations indicated that surfen bound to glycosaminoglycans, and extent binding increased according charge density in order heparin > dermatan sulfate chondroitin sulfate. All charged groups ( N -sulfates, O carboxyl groups) contributed binding, consistent with idea interacted electrostatically. Surfen neutralized...
Autoimmune diseases are characterized by spontaneously occurring autoantibodies which have proven to be useful reagents for the characterization of specific nuclear proteins. Using a monoclonal autoantibody (72B9) derived from murine lupus strain, we cloned cDNA human T-cell line MOLT-4, encodes lamin B. The identity encoded protein as B was established both biochemical and immunological criteria. Inspection deduced amino acid sequence revealed presence in coil 1B alpha-helical domain...
Abstract We isolated a VH1 germline gene (Humhv 1263) that is closely related to the heavy chains of 2 human rheumatoid factors (RF), Bor and Kas. also found 783 rearranged actually 91–93% homologous Kas, it differs from complementary DNA (51P1) by only single base. Thus, likely be unmutated form V H may encode RF Kas several other monoclonal RF.
We show that SARS-CoV-2 spike protein interacts with cell surface heparan sulfate and angiotensin converting enzyme 2 (ACE2) through its Receptor Binding Domain. Docking studies suggest a putative heparin/heparan sulfate-binding site adjacent to the domain binds ACE2. In vitro, binding of ACE2 heparin ectodomains occurs independently ternary complex can be generated using as template. Contrary purified components, on cells codependently. Unfractionated heparin, non-anticoagulant treatment...
Abstract Purpose: The binding of hematogenously borne malignant cells that express the carbohydrate sialyl Lewis X (sLeX) to selectin adhesion receptors on leukocytes, platelets, and endothelial facilitates metastasis. glycosylation inhibitor, per-O-acetylated GlcNAcβ1,3Galβ-O-naphthalenemethanol (AcGnG-NM), inhibits biosynthesis sLeX in tumor cells. To evaluate efficacy AcGnG-NM as an antimetastatic agent, we examined its effect experimental metastasis spontaneous hematogenous dissemination...
Using mRNA from cultured human mesothelial cells, we constructed bacterial plasmids and lambda phage vectors that contained cDNA sequences specific for the keratins expressed in these cells. A cloned encoding keratin K7 (55 kD) was identified by positive hybrid selection. Southern Blot analysis indicated this sequence is represented only once genome, Northern demonstrated gene abundance bronchial but weakly epidermal cells not at all liver, colon, or exocervical tissue. The predicted amino...
Simple epithelial cells synthesize a different set of keratins than epidermal cells. In experiments reported in this manuscript, we show that the base level keratin expression simple is variable for cell types, and that, some epithelia, can be upregulated by increasing exposure to retinoids, but not glucocorticoids or estradiol. To elucidate molecular mechanisms underlying gene regulation, have isolated characterized human encoding K7. By examining possible regulatory elements investigating...
Human fetal muscles at ages 110, 125, and 132 days contain a fetal-specific myosin light chain. This chain is absent in adult human muscle, copurifies with myosin, identified as slow because it reacts purified antibody to chicken muscle chains does not react strongly fast chains. synthesized cultures of along normal The presence culture provides marker for studies disease which important know when or if the makes transition from embryonic expression true phenotype.
The increasing cost of high-volume cultures and dependence on serum growth factor supplementation limit the affordability mesenchymal stromal cell (MSC) therapies. This has spurred interest in developing strategies that support adherent expansion while reducing raw material costs. Culture surfaces coated with sulfated glycosaminoglycans (GAGs), specifically heparan sulfate (HS), are an alternative to prolong retention cultures. Unlike heparin, recombinant HS (rHS) offers strong binding...
Autoimmune diseases are characterized by spontaneously occurring autoantibodies which have proven to be useful reagents for the characterization of specific nuclear proteins. Using a monoclonal autoantibody (72B9) derived from murine lupus strain, we cloned cDNA human T-cell line MOLT-4, encodes lamin B. The identity encoded protein as B was established both biochemical and immunological criteria. Inspection deduced amino acid sequence revealed presence in coil 1B alpha-helical domain...