A5002079653- Cancer Cells and Metastasis
- Mesenchymal stem cell research
- Proteoglycans and glycosaminoglycans research
- Periodontal Regeneration and Treatments
- Cell Adhesion Molecules Research
- Polymer Surface Interaction Studies
- Protease and Inhibitor Mechanisms
- Hedgehog Signaling Pathway Studies
University of Puerto Rico-Mayaguez
2020-2024
Moffitt Cancer Center
2024
The increasing cost of high-volume cultures and dependence on serum growth factor supplementation limit the affordability mesenchymal stromal cell (MSC) therapies. This has spurred interest in developing strategies that support adherent expansion while reducing raw material costs. Culture surfaces coated with sulfated glycosaminoglycans (GAGs), specifically heparan sulfate (HS), are an alternative to prolong retention cultures. Unlike heparin, recombinant HS (rHS) offers strong binding...
Abstract The therapeutic potential of human mesenchymal stromal cells (h‐MSC) is dependent on the viability and secretory capacity both modulated by culture environment. Our previous studies introduced heparin collagen I (HEP/COL) alternating stacked layers as a substrate to enhance secretion immunosuppressive factors h‐MSCs. Herein, we examined impact HEP/COL multilayers growth, morphology, secretome bone marrow adipose‐derived physicochemical properties stability coatings were confirmed at...
Understanding mesenchymal stromal cells (MSCs) growth mechanisms in response to surface chemistries is essential optimize culture methods for high-quality and robust cell yields manufacturing applications. Heparin (HEP) collagen 1 (COL) substrates have been reported enhance adhesion, growth, viability, secretory potential MSCs. However, the biomolecular underlying benefits of combined HEP/COL are unknown. This work used bilayered surfaces investigate role integrin-HEP interactions advantages...
Abstract Preclinical models of Sonic Hedgehog (SHH)-driven tumors support the therapeutic benefit pharmacological inhibitors, yet clinical outcomes are conflicted due to biphasic tumor responses that include complete remission or faster disease progression. In such studies, activity SHH pathway in tumor-adjacent stroma supported restrained Our prior studies show altered ligand levels can lead growth during inhibition suggesting a role for strength triple-negative breast cancer (TNBC) model....