A5040084135- Monoclonal and Polyclonal Antibodies Research
- Systemic Lupus Erythematosus Research
- RNA Research and Splicing
- MicroRNA in disease regulation
- Inflammatory Myopathies and Dermatomyositis
- RNA and protein synthesis mechanisms
- T-cell and B-cell Immunology
- Glycosylation and Glycoproteins Research
- RNA modifications and cancer
- Salivary Gland Disorders and Functions
- Systemic Sclerosis and Related Diseases
- Cytomegalovirus and herpesvirus research
- Lymphoma Diagnosis and Treatment
- Cancer-related molecular mechanisms research
- Neuroblastoma Research and Treatments
- Telomeres, Telomerase, and Senescence
- RNA Interference and Gene Delivery
- Eosinophilic Disorders and Syndromes
- Biochemical and Molecular Research
- Chronic Lymphocytic Leukemia Research
- Viral Infections and Immunology Research
- Immunodeficiency and Autoimmune Disorders
- Diabetes and associated disorders
- Cellular transport and secretion
- Cell Adhesion Molecules Research
University of Florida
2016-2025
University of Florida Health Science Center
2004-2024
Florida College
2003-2022
Stony Brook University
2005-2017
Universidade Federal de São Paulo
2017
Janssen (United Kingdom)
2016
Stony Brook Medicine
2015
University of Calgary
1980-2014
Christian Health Association of Nigeria
2014
Nova Medical (United States)
2014
Abstract Introduction MicroRNAs are small noncoding RNA molecules that negatively regulate gene expression via degradation or translational repression of their targeted mRNAs. It is known aberrant microRNA can play important roles in cancer, but the role microRNAs autoimmune diseases only beginning to emerge. In this study, selected examined rheumatoid arthritis. Methods Total was isolated from peripheral blood mononuclear cells obtained patients with arthritis, and healthy disease control...
Anti-nuclear antibodies (ANA) are fundamental for the diagnosis of autoimmune diseases, and have been determined by indirect immunofluorescence assay (IIFA) decades. As demand ANA testing increased, alternative techniques were developed challenging classic IIFA. These platforms differ in their antigen profiles, sensitivity specificity, raising uncertainties regarding standardisation interpretation incongruent results. Therefore, an international group experts has created recommendations...
Translation initiation on poliovirus RNA occurs by internal binding of ribosomes to a sequence within the 5' untranslated region. We have previously characterized HeLa cell protein, p52, that binds fragment region (K. Meerovitch, J. Pelletier, and N. Sonenberg, Genes Dev. 3:1026-1034, 1989). Here we report purification p52. Protein microsequencing identified p52 as La autoantigen. The protein is human antigen recognized antibodies from patients with autoimmune disorders such systemic lupus...
To estimate the prevalence, types, and sociodemographic biobehavioral correlates of antinuclear antibodies (ANAs) in US.
Anti-SS-A/Ro autoantibodies are found in the sera of patients with Sjogren's syndrome (SS) and SLE. In course analyzing 61 SS for their autoantibody profiles, we that 42 were positive anti-SS-A by double diffusion agarose demonstrated precipitin lines identical to produced a prototype serum. Further analysis these SS-A antibody-positive Western blotting cell extracts revealed 21 reacted two proteins 60 52 kD, 13 52-kD protein, detected only while six nonreactive. Affinity-purified anti-60-kD...
The human toll-like receptor 4 (TLR4) pathway is activated in response to lipopolysaccharide (LPS), and subsequent signal transductions lead the production of cytokines such as tumor necrosis factor-α (TNF-α) by innate immune cells. Defects may contribute overproduction TNF-α leading systemic inflammation diseases. Thus, needs be tightly regulated elaborate mechanisms control its onset termination. LPS tolerance a state hyporesponsiveness challenge achieved monocytic cells after prolonged...
Antibodies producing an unusual immunofluorescent pattern were identified in the sera of patients with diverse autoimmune features. This was characterized by presence up to six round discrete nuclear bodies interphase cell nuclei. Immunoblotting analysis showed that these recognized 80-kD protein, and affinity-purified anti-p80 antibody from protein band reproduced fluorescent staining bodies. Colloidal gold immunoelectron microscopy coiled body, ultramicroscopic structure probably first...
The indirect immunofluorescence assay (IIFA) on HEp-2 cells is widely used for detection of antinuclear antibodies (ANA). dichotomous outcome, negative or positive, integrated in diagnostic and classification criteria several systemic autoimmune diseases. However, the IIFA test has much more to offer: besides titre fluorescence intensity, it also provides pattern(s). latter include nucleus cytoplasm interphase as well patterns associated with mitotic cells. International Consensus ANA...
Growing evidence suggests increasing frequencies of autoimmunity and certain autoimmune diseases, but findings are limited by the lack systematic data evolving approaches definitions. This study was undertaken to investigate whether prevalence antinuclear antibodies (ANA), most common biomarker autoimmunity, changed over a recent 25-year span in US.Serum ANA were measured standard indirect immunofluorescence assays on HEp-2 cells 14,211 participants age ≥12 years from National Health...
ABSTRACT Periodontitis (PD) is a polymicrobial dysbiotic immuno-inflammatory disease. Toll-like receptors (TLRs) are present on gingival epithelial cells and recognize pathogen-associated molecular patterns (PAMPs) pathogenic bacteria, induce the secretion of proinflammatory cytokines, initiate innate adaptive antigen-specific immune responses to eradicate invading microbes. Since PD chronic inflammatory disease, TLR2/TLR4 plays vital role in disease pathogenesis maintaining periodontium...
A novel human cellular structure has been identified that contains a unique autoimmune antigen and multiple messenger RNAs. This complex was discovered using an serum from patient with motor sensory neuropathy protein of 182 kDa. The gene cDNA encoding the indicated open reading frame glycine-tryptophan (GW) repeats single RNA recognition motif. Both patient's rabbit raised against recombinant GW costained discrete cytoplasmic speckles designated as bodies (GWBs) do not overlap Golgi...
Serum SS-A/Ro autoantibodies are commonly found in patients with Sjogren's syndrome, systemic lupus erythematosus, neonatal lupus, and subacute cutaneous lupus. Two proteins of 60 52 kD have been described as targets for these autoantibodies. To define the 52-kD component unambiguously, cDNA clones were isolated from human HepG2 MOLT-4 cell libraries. The identity was established by (a) specificity antibody affinity purified recombinant protein, (b) reactivity protein prototype sera...
Rab11a is a small GTP-binding protein enriched in the pericentriolar plasma membrane recycling systems. We hypothesized that Rab11a-binding proteins exist as downstream effectors of its action. Here we define family four Rab11-interacting proteins: Rab11-Family Interacting Protein 1 (Rab11-FIP1), InteractingProtein 2 (Rab11-FIP2), Rab11-FamilyInteracting 3 (Rab11-FIP3), and pp75/Rip11. All interacting associated with wild type dominant active (Rab11aS20V) well Rab11b Rab25. Rab11-FIP2 also...