A5102985472- Monoclonal and Polyclonal Antibodies Research
- Systemic Lupus Erythematosus Research
- Vasculitis and related conditions
- T-cell and B-cell Immunology
- Systemic Sclerosis and Related Diseases
- Glycosylation and Glycoproteins Research
- Nuclear Structure and Function
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Renal Diseases and Glomerulopathies
- Galectins and Cancer Biology
- Inflammatory Myopathies and Dermatomyositis
- Advanced Biosensing Techniques and Applications
- Case Reports on Hematomas
- Skin Diseases and Diabetes
- Virus-based gene therapy research
- Sarcoidosis and Beryllium Toxicity Research
- Advanced biosensing and bioanalysis techniques
- Infections and bacterial resistance
- Biosensors and Analytical Detection
- Complement system in diseases
- Cancer Diagnosis and Treatment
- Liver Diseases and Immunity
- IgG4-Related and Inflammatory Diseases
- Cerebral Venous Sinus Thrombosis
- Parathyroid Disorders and Treatments
University of New Mexico
2006-2023
Raymond G. Murphy VA Medical Center
2012-2023
New Mexico VA Health Care System
2008-2021
University of Maryland, College Park
2016
Hadassah Medical Center
2016
University of Natural Medicine
2013
Scripps Research Institute
1994-1996
Bispebjerg Hospital
1990
New York Academy of Medicine
1986-1989
Anti-nuclear antibodies (ANA) are fundamental for the diagnosis of autoimmune diseases, and have been determined by indirect immunofluorescence assay (IIFA) decades. As demand ANA testing increased, alternative techniques were developed challenging classic IIFA. These platforms differ in their antigen profiles, sensitivity specificity, raising uncertainties regarding standardisation interpretation incongruent results. Therefore, an international group experts has created recommendations...
We have identified and partially characterized the autoantibodies in sera of 60 patients with chronic fatigue syndrome. Approximately 52% were found to react nuclear envelope antigens. The combination rim staining observed immunofluorescence microscopy immunoblot analysis highly purified proteins provided initial characterization these autoantibodies. Further showed that some immunoprecipitated vitro transcription translation product a human cDNA clone encoding protein lamin B1. IgG isotype....
From a panel of monoclonal antibodies raised against fractions rat liver nuclear envelopes (NEs), we have identified an antibody, RL30, which reacts with novel pore complex (NPC) antigens that are not O-glycosylated. By immunofluorescence staining cultured cells, RL30 exclusively the NE in punctate pattern largely coincides NPC proteins. labels only cytoplasmic surface immunogold electron microscopy, predominantly peripheral regions nearby ring. In immunoblots isolated NEs and recognizes...
Rheumatoid arthritis (RA) is a complex autoimmune disease with poorly understood pathogenesis. The associated polyclonal B cell activation and the production of autoantibodies (autoAbs), but there longstanding controversy as to whether such Abs contribute to, or are secondary pathogenesis RA. To address potential pathogenicity human RA–associated Abs, we developed passive transfer model involving mice deficient in low-affinity inhibitory Fc receptor, FcγRIIB. We report that plasma serum from...
A 54-year-old man was diagnosed with Streptococcus mutans endocarditis of the mitral valve. Serological tests disclosed presence multiple autoantibodies including c-ANCA, anti-PR3 and anti-MPO. While fever subsided antibiotics, mental status renal function deteriorated rapidly. Kidney biopsy revealed pauci-immune glomerulonephritis acute eosinophilic interstitial nephritis. The abnormal clinical features improved rapidly after addition corticosteroids cyclophosphamide to antibiotics....
We report a patient with scleroderma, renal cell carcinoma (RCC) and membranous nephropathy (MN). Certain clinical laboratory features suggested that RCC caused or enhanced the other two conditions. A 55-year-old man developed scleroderma which progressed rapidly during its first 2 years development of hypertension acute failure, peak serum creatinine (SCr) 327 micromol/l (3.7 mg/dl) partial improvement function (SCr 239 2.7 after initiation an angiotensin converting enzyme inhibitor. He...
Testing for total antinuclear antibodies (ANA) is a critical tool diagnosis and management of autoimmune diseases at both the primary care subspecialty settings. Repurposing ANA from test lupus to any condition has driven increase in requests. Changes referral patterns include early or subclinical disease detection patients with low pre-test probability use negative results rule out underlying disease. A positive result can lead further diagnostic considerations. Currently, tests are...
We present a patient with systemic symptoms including 4 months of dyspnoea worsened exertion, fatigue, rhinorrhoea, intermittent facial swelling, generalised lymphadenopathy and weight loss. Laboratory studies demonstrated proteinuria eosinophilia. His serology was consistent Epstein-Barr Virus (EBV) reactivation. A lymph node biopsy EBV-associated reactive lymphoid hyperplasia. He told to continue symptomatic treatment for EBV infection. After several admissions, vasculitis workup...
Tumoral calcinosis (TC) is rare in patients with systemic sclerosis but associated morbidity. Paraspinal TC may cause severe pain and potentially devastating neurological deficits. Surgical decompression by removing the masses applying surgical techniques to support spine have provided substantial relief of symptoms majority cases. However, death has occurred immediate postoperative period can even occur after several months. Current indications for surgery include intractable neck and, most...
Journal Article Evidence for absence of histones in the Crithidia luciliae kinetoplast: a study with anti‐H2A and monoclonal anti‐H3 antibodies Get access K.N. KONSTANTINOV, KONSTANTINOV Institute Dermatology Venerology, Academy Medicine, Sofia Dr Konstantin Konstantinov, Boul. Georgi Sofiiski 1, 1431 Sofia, Bulgaria. Search other works by this author on: Oxford Academic Google Scholar VALYA R. RUSSANOVA Molecular Biology, Bulgarian Sciences, Bulgaria British Dermatology, Volume 117, Issue...