A5024659496- Reproductive System and Pregnancy
- Systemic Lupus Erythematosus Research
- Monoclonal and Polyclonal Antibodies Research
- Parvovirus B19 Infection Studies
- Prenatal Screening and Diagnostics
- Chronic Lymphocytic Leukemia Research
- Cytomegalovirus and herpesvirus research
- Pregnancy and Medication Impact
- COVID-19 Clinical Research Studies
- SARS-CoV-2 and COVID-19 Research
- Vasculitis and related conditions
- Systemic Sclerosis and Related Diseases
- COVID-19 epidemiological studies
- Renal and related cancers
- Biliary and Gastrointestinal Fistulas
- Enterobacteriaceae and Cronobacter Research
- Esophageal and GI Pathology
- Fetal and Pediatric Neurological Disorders
- Diabetes and associated disorders
- Herpesvirus Infections and Treatments
- Cancer Genomics and Diagnostics
- Infectious Disease Case Reports and Treatments
- Hepatitis C virus research
- Molecular Biology Techniques and Applications
- Reproductive tract infections research
Hospital do Desterro
2023
Hospital CUF Descobertas
2023
Portuguese Army
2019-2023
Lusíada University of Lisbon
2021
University of Lisbon
2021
Los Alamos National Laboratory
2021
IPO Porto
2019-2020
Université Paris-Sud
2016
Hôpital Antoine-Béclère
2016
Université Libre de Bruxelles
2016
Anti-nuclear antibodies (ANA) are fundamental for the diagnosis of autoimmune diseases, and have been determined by indirect immunofluorescence assay (IIFA) decades. As demand ANA testing increased, alternative techniques were developed challenging classic IIFA. These platforms differ in their antigen profiles, sensitivity specificity, raising uncertainties regarding standardisation interpretation incongruent results. Therefore, an international group experts has created recommendations...
B cells play a role in pregnancy due to their humoral and regulatory activities. To our knowledge, different maturational stages (from transitional memory) of circulating cell subsets have not yet been characterized (cell quantification phenotype identification) healthy pregnant women. Thus, the objective study was characterize these (as well as cells) from late post-partum compare them with non-pregnant In all enrolled women, flow cytometry used according expression IgD CD38 (Bm1-Bm5...
During the COVID-19 pandemic, Portugal has experienced three distinct SARS-CoV-2 infection waves. We previously documented prevalence of immunity, measured by specific antibodies, in September 2020, 6 months after initial moderate wave. Here, we show seroprevalence changes later, up to second week March 2021, shortly following third wave, which was one most severe world, and 2 start vaccination campaign. A longitudinal epidemiological study conducted, with a stratified quota sample...
Abstract Special conditions associated with laboratory autoimmune testing are not well compatible recent developments in regulatory frameworks such as EN/ISO 15189 accreditation or vitro diagnostic medical device regulation (IVD-R). In addition, international recommendations, guidelines and disease criteria poorly defined respect to requirements on autoantibody testing. Laboratory specialists from Austria, Belgium, Croatia, Estonia, Finland, France, Germany, Greece, Hungary, Italy, Norway,...
The International Consensus on Antinuclear Antibody (ANA) Patterns (ICAP) has recently proposed nomenclature in order to harmonize ANA indirect immunofluorescence (IIF) pattern reporting. ICAP distinguishes competent-level from expert-level patterns. A survey was organized evaluate reporting, familiarity, and considered clinical value of IIF patterns.Two surveys were distributed by European Autoimmunity Standardization Initiative (EASI) working groups, the UK NEQAS laboratory professionals...
Lawsonella clevelandensis is a Gram-stain-positive, partially acid-fast, anaerobic, being considered new species within genus in the suborder Corynebacterineae. There are only few cases reported worldwide. This fastidious microorganism, difficult to identify by conventional methods, leading inappropriate treatments. The authors report case of 29-year-old woman with 3-week evolution breast nodule. was family history carcinoma. Samples were collected for histological and microbiological...
Cytomegalovirus (CMV) is the most frequent cause of congenital infection all over world. Its prevalence ranges from 0.2 to 2.2%. Transmission children their pregnant mothers a well-known risk factor, particularly if they attend childcare centre. This study aims compare CMV (CMV_CI) in Portugal (Lisbon) between two studies, performed respectively 2019 and 2020. In study, hospitals, we found 0.67% CMV_CI prevalence, using pool strategy previously tested with saliva samples. 2020 same approach...
To evaluate the occurrence of plasmid-mediated fos genes among fosfomycin-resistant Escherichia coli isolates collected from patients in Lisbon, Portugal, and characterize fos-positive strains. A total 19,186 E. were prospectively between April 2022 January 2023 inpatients outpatients at a private laboratory Lisbon. Fosfomycin resistance was initially assessed by semi-automated systems further confirmed disc diffusion method. Resistant investigated for (fosA1-fosA10, fosC fosL1-fosL2)...
Abstract Human cytomegalovirus (HCMV) is the leading congenital infection agent in world. The importance of screening this has been debated, as 10–15% asymptomatic newborns with HCMV at birth will present late sequelae. aim study was to test feasibility using saliva pools from a program for infection, two Portuguese hospitals. based on use 10 samples detection viral DNA by real-time PCR. Whenever there positive pool, were tested individually, and each sample result confirmed urine collected...
Abstract Background: Detection of anti-neutrophil cytoplasmic antibodies (ANCA) is important for the diagnosis ANCA-associated vasculitides (AAV). For AAV, especially ANCA directed against myeloperoxidase (MPO) and proteinase 3 (PR3) are most relevant. with less well-defined specificities may, however, also be detected in other inflammatory non-inflammatory conditions. Methods: A questionnaire, initiated by European Autoimmunity Standardisation Initiative (EASI), was used to gather...
In September 2020, we tested 13,398 persons in Portugal for antibodies against severe acute respiratory syndrome coronavirus 2 by using a quota sample stratified age and population density. We found seroprevalence of 2.2%, 3-4 times larger than the official number cases at end first wave pandemic.
Prenatal diagnosis of fetal trisomy 21 and other chromosomal abnormalities is based on invasive tests, such as amniocentesis chorionic villus sampling, which are carried out in women identified through screening being at high risk for these abnormalities. The most widely used method the first-trimester combined test utilizes maternal age, measurements nuchal translucency thickness (NT) serum pregnancy-associated plasma protein-A (PAPP-A) free β-human gonadotropin (hCG). To assess influence...
Objective: The rapid increase of cell-free fetal DNA analysis for Down syndrome screening requires evidence-based clinical practice guidelines noninvasive prenatal testing (NIPT). Several studies show that the quality many is low and there are still health areas where this not systematically evaluated. Given absence research, in NIPT field, we used an internationally validated tool to evaluate a set three look at dimensions can be improved.Methods: Four appraisers, experts screening,...
Background: Cytomegalovirus is the most common cause of congenital infections worldwide. Screening all newborns in first 2 weeks life only way to detect cases infection, allowing monitoring children with asymptomatic infection at birth and early intervention. Aim: In this multicenter study, we aimed evaluate feasibility using a saliva pool strategy for mass screening 7 Portuguese hospitals, estimate current prevalence these hospitals. Methods: A total 7033 were screened between June 2020...