A5067054819- Monoclonal and Polyclonal Antibodies Research
- Toxoplasma gondii Research Studies
- Systemic Lupus Erythematosus Research
- Cytomegalovirus and herpesvirus research
- Herpesvirus Infections and Treatments
- Multiple Myeloma Research and Treatments
- Chronic Lymphocytic Leukemia Research
- Immunodeficiency and Autoimmune Disorders
- Blood groups and transfusion
- Reproductive System and Pregnancy
- Amyloidosis: Diagnosis, Treatment, Outcomes
- Pancreatic function and diabetes
- Diabetes Management and Research
- Reproductive Biology and Fertility
- Bacteriophages and microbial interactions
- Rabies epidemiology and control
- Diabetes and associated disorders
- Hemoglobinopathies and Related Disorders
- Vasculitis and related conditions
- Multiple Sclerosis Research Studies
- Cystic Fibrosis Research Advances
- Vehicle emissions and performance
- Platelet Disorders and Treatments
- Parasitic Infections and Diagnostics
- Air Quality Monitoring and Forecasting
AZ Sint-Jan
2017-2025
Vrije Universiteit Brussel
2009-2025
Universitair Ziekenhuis Brussel
2010-2018
Palmetto Hematology Oncology
2012-2016
Bipar
1987-2011
Institut Belge pour la Sécurité Routière
1997
Objective: To validate kappa free light chain (KFLC) and lambda (LFLC) indices as a diagnostic biomarker in multiple sclerosis (MS). Methods: We performed multicenter study including 745 patients from 18 centers (219 controls 526 clinically isolated syndrome (CIS)/MS patients) with known oligoclonal IgG band (OCB) status. KFLC LFLC were measured paired cerebrospinal fluid (CSF) serum samples. Gaussian mixture modeling was used to define cut-off for indexes. Results: The the index 6.6 (95%...
ABSTRACT C57BL/6, C3H, and BALB/c mice were vaccinated with plasmids encoding Toxoplasma gondii antigens GRA1, GRA7, ROP2, previously described as strong inducers of immunity. Seroconversion for the relevant antigen was obtained in majority animals. T. lysate stimulated specific T-cell proliferation secretion gamma interferon (IFN-γ) spleen cell cultures from C3H but not those control mice. Although proliferating, splenocytes DNA-vaccinated C57BL/6 also produced IFN-γ. No interleukin-4...
Abstract Objectives Antinuclear antibodies (ANA) are important for the diagnosis of various autoimmune diseases. ANA usually detected by indirect immunofluorescence assay (IFA) using HEp-2 cells (HEp-2 IFA). There many variables influencing IFA results, such as subjective visual reading, serum screening dilution, substrate manufacturing, microscope components and conjugate. Newer developments on testing that offer novel features adopted some clinical laboratories include automated...
Abstract Human leukocyte Ag-G, a tolerogenic molecule that acts on cells of both innate and adaptive immunity, plays an important role in tumor progression, transplantation, placentation, as well the protection allogeneic fetus from maternal immune system. We investigated HLA-G mRNA protein expression human embryonic stem (hESC) derived inner cell mass (ICM) blastocysts. hESC self-renew indefinitely culture while maintaining pluripotency, providing unlimited source for therapy. was present...
ABSTRACT Dense granule protein 7 (GRA7) of Toxoplasma gondii was expressed in Escherichia coli as a fusion protein. The leader peptide contained 25-amino-acid mouse tumor necrosis factor fragment and six histidyl residues. After purification by metal chelate affinity chromatography, the antigen evaluated an enzyme-linked immunosorbent assay for detection immunoglobulin G (IgG). For two sets IgG-positive human serum samples, obtained from routine screening, overall sensitivity 81% obtained....
Protective immunity against Toxoplasma gondii is known to be mediated mainly by T lymphocytes and gamma interferon (IFN-gamma). The contribution of CD4(+) CD8(+) T-lymphocyte subsets protective immune responses T. infection, triggered a GRA1 (p24) DNA vaccine, was assessed in this study. In vitro T-cell depletion experiments indicated that both produced IFN-gamma upon restimulation with lysate. addition, the vaccine elicited cells were shown have cytolytic activity parasite-infected target...
Antineutrophil cytoplasmic antibodies (ANCA) are valuable laboratory markers to support the diagnosis of ANCAassociated vasculitis (AAV).High-quality immunoassays for proteinase-3 (PR3)-ANCA and myeloperoxidase (MPO)-
Background Cerebrospinal fluid (CSF) kappa free light chain (κFLC) measures gained increasing interest as diagnostic markers in multiple sclerosis (MS). However, the lack of studies comparing assay-dependent cutoff values hinders their use clinical practice. Additionally, optimal κFLC parameter for identifying MS remains a subject ongoing debate. Objectives The aim this study was to compare same-sample accuracies index, κIgG CSF κFLC/IgG ratio, and isolated (iCSF-κFLC) between two reference...
Free light chain (FLC) measurement gained a lot of interest for diagnostic workup monoclonal gammopathy. We evaluated the performance turbidimetric polyclonal Freelite (The Binding Site, Birmingham, UK) assays on Cobas 6000 (Roche Diagnostics, Rotkreuz, Switzerland) and nephelometric N Latex (Siemens Healthcare Marburg, Germany) BN ProSpec (Dade Behring, Deerfield, IL) vs established ProSpec. Analytical was acceptable. Method comparison (n = 118) showed significant proportional FLC...
The International Consensus on Antinuclear Antibody (ANA) Patterns (ICAP) has recently proposed nomenclature in order to harmonize ANA indirect immunofluorescence (IIF) pattern reporting. ICAP distinguishes competent-level from expert-level patterns. A survey was organized evaluate reporting, familiarity, and considered clinical value of IIF patterns.Two surveys were distributed by European Autoimmunity Standardization Initiative (EASI) working groups, the UK NEQAS laboratory professionals...
Autoantibodies to nuclear antigens, i.e. antinuclear antibodies (ANA), double-stranded DNA (dsDNA) and extractable antigens (ENA), are useful as diagnostic markers for a variety of autoimmune diseases. In March 2010, the Belgian national External Quality Assessment Scheme sent questionnaire on ANA, anti-dsDNA anti-ENA antibody testing designed by Dutch EASI (European Autoimmunity Standardization Initiative) team, all clinical laboratories performing ANA testing. Virtually completed (97·7%,...
ABSTRACT Murine antibody responses to soluble proteins are generally restricted the immunoglobulin G1 (IgG1) isotype. When mice were infected with Toxoplasma gondii Beverley and concomitantly immunized a unrelated protein antigen, modification in isotypic distribution of antibodies directed against this nonparasite antigen was observed, preferential production IgG2a. Interestingly, when during chronic phase (day 40) infection T. Beverley, similar antiprotein observed.
SUMMARY We have recently shown that Toxoplasma gondii tachyzoites grown in vitro culture can bind unspecific immunoglobulin (Ig) through their Fc moiety. show now receptors are also present on T. within the host animal, and intraperitoneal parasites immunocompetent mice saturated with Ig. investigated effect of parasite's receptor interaction mammalian cells, using Vero cell line as a model for nonphagocytic cells murine peritoneal macrophages primary phagocytic cells. Coating...