A5030695428- Tuberculosis Research and Epidemiology
- Mycobacterium research and diagnosis
- Reproductive tract infections research
- Syphilis Diagnosis and Treatment
- Urinary and Genital Oncology Studies
- Infectious Diseases and Tuberculosis
- Infectious Diseases and Mycology
- Pneumocystis jirovecii pneumonia detection and treatment
- Genetics, Bioinformatics, and Biomedical Research
- Pneumonia and Respiratory Infections
- Diagnosis and treatment of tuberculosis
- Cervical Cancer and HPV Research
- Respiratory viral infections research
- Urinary Tract Infections Management
- Liver Disease Diagnosis and Treatment
- Liver Disease and Transplantation
- Parasitic Infections and Diagnostics
- Genital Health and Disease
Administração Regional de Saúde de Lisboa e Vale do Tejo
2024-2025
Hospital Prof. Dr. Fernando Fonseca
2004-2015
Background Lymphogranuloma venereum (LGV) is a sexually transmitted infection caused by Chlamydia trachomatis ompA -genotypes L1–L3, with increasing numbers of detected cases across Europe. Here, we analysed diversity and temporal distribution the LGV -subvariants in Portugal between 2007 2023, order to better understand dissemination diversification landscape strains. Methods The collection Portuguese National Reference Laboratory includes 1188 -genotyped samples 2023. In-depth analysis...
Objectives Chlamydia trachomatis is classified into 15 major genotypes, A to L3, based on the diversity of ompA gene. Here, we evaluated and characterised distribution -genotypes over 32 years (1990–2021) in Portugal. Methods The collection Portuguese National Reference Laboratory for Sexually Transmitted Infections includes 5824 C . -positive samples that were successfully -genotyped between 1990 2021. An in-depth analysis across years, as well by biological sex, age anatomical site...
To survey genetic markers of potential antimicrobial resistance (AMR) to macrolides and fluoroquinolones among Chlamydia trachomatis-positive samples from the collection Portuguese National Reference Laboratory for Sexually Transmitted Infections (STIs), explore a multiplex PCR approach coupled with NGS provide complementary information regarding strain's genomic backbone. A total 502 C. samples, mostly anorectal exudates, were subjected sequencing five targets, including loci potentially...
Major contributions from pathogen genome analysis and host genetics have equated the possibility of Mycobacterium tuberculosis co-evolution with its human leading to more stable sympatric host-pathogen relationships. However, attribution either or allopatric categories depends on resolution grain genotypic characterization. We explored influence relationship clinical (HIV infection multidrug-resistant [MDRTB]) demographic (gender age) factors in regards by using spacer oligonucleotide typing...
<title>Abstract</title> <bold>Background</bold> Lymphogranuloma venereum is a sexually transmitted infection caused by <italic>Chlamydia trachomatis ompA</italic>-genotypes L1-L3, with increasing numbers of detected cases across Europe. Here, we analysed diversity and temporal distribution the LGV <italic>ompA</italic>-subvariants in Portugal between 2007 2023, order to better understand dissemination diversification landscape strains.<bold>Methods</bold> The collection Portuguese National...
O Spoligotyping foi utilizado na genotipagem de 219 isolados do complexo Mycobacterium tuberculosis doentes Hospital Fernando Fonseca. Esta técnica, baseada metodologia PCR, analisa uma região cromossómica específica M. tuberculosis, o locus DR (Direct Repeats). Com auxílio base dados internacional, mostramos que 29,2 % dos Spoligotypes predominantes pertenciam à família LAM (Latino-American Mediterranean). A 9, com 12,3 %, deixou-nos atentos possível importação da doença populações oriundas...
O diagnóstico diferencial do Mycobacterium bovis é importante no controlo da transmissão para a população humana e também tratamento doença, visto que o M. naturalmente resistente à pirazinamida. Foram analisados onze isolados clínicos Hospital Fernando Fonseca, com um padrão de Spoligotype indicativo pela ausência dos espaçadores 39-43, mas contaram espaçador 38. Para identificação destas estirpes procedeu-se análise fenotípica resistência pirazinamida estudo polimorfismos genes pncA gyrB....
O presente estudo populacional, que decorreu entre 1999 e 2003, foi baseado na utilização do Spoligotyping genotipagem de 452 isolados complexo Mycobacterium tuberculosis doentes com tuberculose internados no Hospital Fernando Fonseca. Spoligotypes foram identificados como shared types (ST) recorrendo a uma base dados internacional. Onze ST raros, não dados, acomodaram 8,4% dos isolados. Aliás, particular Portugal poderá ser predominância mas previamente classificados famílias genotípicas,...
Introduction The prevalence of nontuberculous mycobacteria (NTM) is higher in patients with structural lung disease and immunocompromised patients. Lung involvement the most common.
<title>Abstract</title> <bold>Objectives: </bold>To survey genetic markers of potential antimicrobial resistance (AMR) to macrolides and fluoroquinolones among <italic>C. trachomatis</italic>-positive samples from the collection Portuguese National Reference Laboratory for STI explore application a multiplex PCR approach coupled with Next-Generation Sequencing (NGS) provide complementary information regarding strain’s genomic backbone. <bold>Methods: </bold>502 were subjected sequencing five...
The diagnosis of tuberculosis is seriously hampered in the absence standard biosafety laboratory facilities for specimen concentration and Mycobacterium culture. Within a twinning arrangement, heat-fixed direct smear sediment from 74 bleach-processed 20 non-processed specimens Cumura Hospital, Guinea-Bissau, were sent to Lisbon molecular evaluation rifampicin resistance. Sequence analysis 369 base-pair rpoB locus detected 3.2% (3/94) resistant specimens. To our knowledge, this represents...