A5026951365- Bone health and osteoporosis research
- Climate Change and Health Impacts
- Heavy metals in environment
- Dental Implant Techniques and Outcomes
- Dental materials and restorations
- Air Quality and Health Impacts
- Heavy Metals in Plants
- Radioactivity and Radon Measurements
- Global Health Care Issues
- Carcinogens and Genotoxicity Assessment
- Bone Metabolism and Diseases
- Biomarkers in Disease Mechanisms
- Diet and metabolism studies
- Orthodontics and Dentofacial Orthopedics
- Endodontics and Root Canal Treatments
- Multiple Myeloma Research and Treatments
- Trace Elements in Health
- Protein Degradation and Inhibitors
- Folate and B Vitamins Research
- Microbial Fuel Cells and Bioremediation
- Hibiscus Plant Research Studies
- Bone health and treatments
- Pericarditis and Cardiac Tamponade
- Respiratory viral infections research
- Adipose Tissue and Metabolism
Escola Superior de Saúde Egas Moniz
2014-2024
Polytechnic Institute of Bragança
2020
University College London
2010-2016
University of Toronto
2009
Canada Research Chairs
2009
Rede de Química e Tecnologia
2007
Instituto Superior Técnico
2003
Instituto Politécnico de Lisboa
2003
Universidade Nova de Lisboa
2003
National Center for Toxicological Research
2001
Following neuropathy α2-adrenoceptor-mediated diffuse noxious inhibitory controls (DNIC), whereby a conditioning stimulus inhibits the activity of spinal wide dynamic range (WDR) neurons, are abolished, and 5-HT7 receptor densities increased. Here, we manipulate 5-HT content in nerve ligated (SNL) animals investigate which mediated actions predominate.Using vivo electrophysiology recorded WDR neuronal responses to von frey filaments applied hind paw before, concurrent to, ear pinch (the...
In Portugal, data on mortality rate attributed to household and ambient air pollution are not reported due shortness irregularity of the available series, therefore, disclosure national progress in reducing number deaths illnesses from contamination exposures multiple pollutants is incomplete. The present work describes application AirQ+ model developed by WHO calculate how much specific health outcomes attributable long-term exposure atmospheric NO2, PM2.5, O3 population various...
Thalidomide (TD) causes birth defects in humans and rabbits via several potential mechanisms, including bioactivation by embryonic prostaglandin H synthase (PHS) enzymes to a reactive intermediate that enhances oxygen species (ROS) formation. We show herein TD rabbit embryo culture produces relevant embryopathies, decreases head/brain development 28% limb bud growth 71% (P<0.05). Two hydrolysis products, 2-phthalimidoglutaramic acid (PGMA) 2-phthalimidoglutaric (PGA), were similarly...
The cytochrome c nitrite reductase is isolated from the membranes of sulfate‐reducing bacterium Desulfovibrio desulfuricans ATCC 27774 as a heterooligomeric complex composed by two subunits (61 kDa and 19 kDa) containing c‐ type hemes, encoded genes nrfA nrfH , respectively. extracted has in average 2NrfA:1NrfH composition. separation ccNiR one another accomplished gel filtration chromatography presence SDS. amino‐acid sequence biochemical characterization show that NrfA contains five hemes...
2,6-Dimethylaniline (2,6-diMeA) is a ubiquitous environmental pollutant that used in industry as synthetic intermediate. It also found tobacco smoke and major metabolite of lidocaine. Although the potential carcinogenicity 2,6-diMeA humans presently uncertain, this aromatic amine has been classified rodent carcinogen. In addition, it known to form hemoglobin adducts humans, which indicates profile metabolic activation similar typical arylamine carcinogens. Like other amines, shown yield...
The role of prostaglandin H synthase-1 (PHS-1) and a related model enzyme, horseradish peroxidase (HRP), in catalyzing the bioactivation dopamine (DA) epinephrine their precursors metabolites to potential neurodegenerative free radical intermediates was examined. To determine contribution PHS-dependent reactive oxygen species (ROS) formation, neurotransmitter DA or its precursor were incubated vitro with purified ovine PHS-1 calf thymus DNA. DA, l-dihydroxyphenylalanine (l-DOPA), precursor,...
Nowadays, food supplements are widely consumed, often without any medical supervision. In this study, 25 for weight loss, randomly purchased from five different suppliers in the European Union, were analysed by Wavelength Dispersive X ray Fluorescence spectrometry (WDXRF). The aim of study was risk assessment trace elements and mixture present loss. obtained Hazard Index (0.11) showed no potential non-carcinogenic effects to human health. However, since humans frequently exposed routes...
Dysphagic patients who underwent endoscopic gastrostomy (PEG) usually present protein-energy malnutrition, but little is known about micronutrient malnutrition. The aim of the study was evaluation serum zinc in and its relationship with proteins, whole blood zinc, nature underlying disorder.From that a sample obtained minutes before procedure. Serum evaluated using Wavelength Dispersive X-ray Fluorescence Spectroscopy. albumin transferrin were evaluated. Patients studied as divided into two...
Due to their polymerization characteristics, hyper-branched dendrimers have lately shown be promising candidates for use in dental materials. In this study, a new adhesive system was prepared, using dendrimer derived from 2-isocyanatoethyl methacrylate (G-IEMA), and its properties were investigated. The exposed dentin treated with four universal adhesives (UAs): SBU (Scotchbond Universal™), FUT (Futurabond M+™), AE1 (experimental Bis-GMA) AE2 G-IEMA), Etch & Rinse (ER) or Self (SE)...
This work demonstrates that an analytical method based on wavelength dispersive X-ray fluorescence spectrometry has acceptable degree of linearity, precision and accuracy in a given range, which make it suitable for the purpose determining Cu, Zn, Fe Cr impurities final drug products.
Product ion spectra from thirteen C8-substituted alkylaniline adducts of guanine and deoxyguanosine were generated using electrospray ionization quadrupole trap mass spectrometry studied to investigate the possibility differentiating isomeric adduct structures based upon relative abundances fragment ions derived alkylaniline-modified bases (BH2+ ions). The structural discrimination BH2+ formed by attachment alkylanilines C8 position is a challenging problem because tend yield product that...
Thalidomide (TD) displays remarkable species specificity, causing birth defects (teratogenesis) in humans and rabbits, but not rats or mice; yet, few determinants of susceptibility have been identified. Also, certain mouse strains are susceptible to the embryopathic effects some teratogens embryo culture despite their resistance vivo. Herein we show that CD-1 embryos resistant limb embryopathies caused by TD two its hydrolysis products, 2-phthalimidoglutaramic acid 2-phthalimidoglutaric...
A vast number of adhesive formulations exist currently. However, available adhesives still have several drawbacks such as increased hydrophilicity, polymerization deficiency, potential cytotoxicity and limited monomer interdiffusion within dentin. To improve material properties, a Bisphenol A-free containing novel dendrimer G(2)-isocyanatoethyl methacrylate (G-IEMA) in replacement Bis-GMA was made tested. Sound human molars were sectioned to expose mid-coronal dentin, which bonded using four...
The ultimate goal of this study was the development a new Bis-GMA-free dental adhesive system with improved properties. Instead Bis-GMA, dendritic macromer (G-IEMA), derived from 2-isocyanatoethyl methacrylate (IEMA), used and its physicochemical properties were investigated. G-IEMA synthesized subsequently incorporated into an experimental (AE2) formulated without Bis-GMA. Double bond conversion (DBC), polymerization shrinkage (PS), water sorption (WS) solubility (SL) evaluated results...
Abstract The elemental composition of chemical elements can vary between healthy and diseased tissues, providing essential insights into metabolic processes in physiological states. This study aimed to evaluate the calcium (Ca) phosphorus (P) levels bones rats with/without streptozotocin-induced diabetes and/or exposure infrasound. X-ray fluorescence spectroscopy was used determine concentrations Ca P Wistar rat tibiae samples. results showed a significant decrease bone concentration...