A5041488438- Protease and Inhibitor Mechanisms
- Peptidase Inhibition and Analysis
- Cell Adhesion Molecules Research
- Skin and Cellular Biology Research
- Immunotherapy and Immune Responses
- TGF-β signaling in diseases
- Nonmelanoma Skin Cancer Studies
- Telomeres, Telomerase, and Senescence
- Systemic Sclerosis and Related Diseases
- Wound Healing and Treatments
- Cancer-related molecular mechanisms research
- Cutaneous Melanoma Detection and Management
- Cutaneous lymphoproliferative disorders research
- Connective tissue disorders research
- Virus-based gene therapy research
- Advanced biosensing and bioanalysis techniques
- Dermatologic Treatments and Research
- Periodontal Regeneration and Treatments
- Blood Coagulation and Thrombosis Mechanisms
- Proteoglycans and glycosaminoglycans research
- NF-κB Signaling Pathways
- Nanoplatforms for cancer theranostics
- Dermatological and Skeletal Disorders
- Plant and Fungal Interactions Research
- Cancer Research and Treatments
Turku University Hospital
2016-2025
University of Turku
2016-2025
Creative Commons
2022
Queen Mary University of London
2015
University of Southern California
2015
St George Hospital
2015
King's College London
2015
Åbo Akademi University
2000-2013
University of Helsinki
1994-2013
Tampere University Hospital
2013
Degradation of basement membranes and stromal extracellular matrix (ECM) is crucial for invasion metastasis malignant cells. ECM initiated by proteinases secreted different cell types participating in tumor invasion, increased expression or activity every known class (metallo-, serine-, aspartic-, cysteine) has been linked to malignancy Studies performed over the last decade have revealed that metalloproteinases (MMPs) play a role invasion. Expression MMP genes transcriptionally regulated...
Wound repair is initiated with the aggregation of platelets, formation a fibrin clot, and release growth factors from activated coagulation pathways, injured cells, extracellular matrix (ECM), followed by migration inflammatory cells to wound site. Thereafter, keratinocytes migrate over wound, angiogenesis initiated, fibroblasts deposit remodel granulation tissue. Cell migration, angiogenesis, degradation provisional matrix, remodeling newly formed tissue, all require controlled ECM....
Thymic peptides, a heterogenous family of polypeptidic hormones synthesized within the thymus, not only exert important regulatory effects both immune and neuroendocrine systems but are also themselves subject to control by derived from hypothalamic-pituitary-adrenal axis (HPA) other endocrine glands. Regarding thymic hormonal function, thymulin production is up-regulated several hormones, including prolactin, growth hormone thyroid hormones. Other aspects physiology epithelial cells can be...
Abstract The term ‘sclerosing diseases of the skin' comprises specific dermatological entities, which have fibrotic changes skin in common. These mostly manifest different clinical subtypes according to cutaneous and extracutaneous involvement can sometimes be difficult distinguish from each other. present guideline focuses on characteristic histopathological features, diagnostic scores serum autoantibodies most useful for differential diagnosis. In addition, current strategies first‐...
The tumor necrosis factor (TNF), Fas, and TNF-related apoptosis-inducing ligand (TRAIL) receptors (R) are highly specific physiological mediators of apoptotic signaling. We observed earlier that a number FasR-insensitive cell lines could redirect the proapoptotic signal to an anti-apoptotic ERK1/2 resulting in inhibition caspase activation. Here we determine similar mechanisms operational regulating signaling other death receptors. Activation FasR, TNF-R1, TRAIL-R, respectively, rapidly...
Collagenase-3 (MMP-13) is a novel matrix metalloproteinase, the expression of which has so far only been documented in human breast carcinomas and osteoarthritic cartilage. In this study we have examined MMP-13 during fetal development. Northern blot hybridizations revealed abundant mRNAs total RNA from cartilage calvaria at gestational age 15 weeks. By situ hybridization transcripts were detected chondrocytes hypertrophic vertebrae spinal column dorsal end ribs undergoing ossification, as...
Collagenase-3 (matrix metalloproteinase-13, MMP-13) is a recently identified human MMP with an exceptionally wide substrate specificity and restricted tissue-specific expression. Here we show that MMP-13 expression induced in normal skin fibroblasts cultured within three-dimensional collagen gel resulting production proteolytic activation of MMP-13. Induction mRNAs by was potently inhibited blocking antibodies against alpha1 alpha2 integrin subunits augmented activating antibody beta1...
A classical model for studying the effects of extracellular matrix is to culture cells inside a three-dimensional collagen gel. When surrounded by fibrillar collagen, many cell types decrease production type I and expression interstitial collagenase (matrix metalloproteinase-1; MMP-1) simultaneously induced. To study role collagen-binding integrins α1β1 α2β1 in this process, we used three different osteogenic lines with distinct patterns putative receptors: HOS cells, which express only...
Proteoglycans (PGs) comprise a group of extracellular matrix macromolecules which play an important role in biology. In this study, normal human skin and gingival fibroblast cultures were incubated with transforming growth factor-beta 1 (TGF-beta 1), the expression three PGs, viz. biglycan (PGI), decorin (PGII), versican (a large proteoglycan) was examined. The results indicate that TGF-beta (5 ng/ml) markedly increased (up to 24-fold) 6-fold) mRNAs enhancement coordinate elevated type I...
ABSTRACT Collagenase-3 (MMP-13) is a human matrix metalloproteinase specifically expressed by transformed squamous epithelial cells, i.e. cell carcinoma (SCC) cells in culture and vivo. Here, we have elucidated the signaling pathways regulating MMP-13 expression epidermal keratinocytes, ras-transformed HaCaT line A-5 cutaneous SCC (UT-SCC-7). Treatment with tumor necrosis factor-α (TNF-α) resulted activation of extracellular signal-regulated kinase (ERK)1,2, Jun N-terminal p38...
Two collagen receptors, integrins α1β1 and α2β1, can regulate distinct functions in cells. Ligation of α1β1, unlike has been shown to result recruitment Shc activation the Ras/ERK pathway. To identify downstream signaling molecules activated by α2β1 integrin, we have overexpressed wild-type α2, or chimeric α2 subunit with α1 integrin cytoplasmic domain human osteosarcoma cells (Saos-2) lacking endogenous α2β1. The α2/α1 chain formed a functional heterodimer β1. In contrast chimera, forced...
Human collagenase-3 (matrix metalloproteinase 13 (MMP-13)) is characterized by exceptionally wide substrate specificity and restricted tissue specific expression. skin fibroblasts in culture express MMP-13 only when they are three-dimensional collagen (Ravanti, L., Heino, J., López-Otı́n, C., Kähäri. V.-M. (1999)J. Biol. Chem. 274, 2446–2455). Here we show that expressed during normal human gingival wound repair. Expression of cultured monolayer or gel was induced transforming growth...
Inflammatory cytokines tumor necrosis factor-alpha and interleukin-1 trigger the ceramide signaling pathway, initiated by neutral sphingomyelinase-elicited hydrolysis of cell membrane phospholipid sphingomyelin to ceramide, a new lipid second messenger. Here, we show that triggering pathway sphingomyelinase or C2- C6-ceramide enhances collagenase-1 (matrix metalloproteinase-1; MMP-1) gene expression fibroblasts. C2-ceramide activates three distinct mitogen-activated protein kinases (MAPKs)...
Degradation of collagenous extracellular matrix by collagenase 1 (also known as metalloproteinase [MMP-1]) plays a role in the pathogenesis various destructive disorders, such rheumatoid arthritis, chronic ulcers, and tumor invasion metastasis. Here, we have investigated distinct mitogen-activated protein kinase (MAPK) pathways regulation MMP-1 gene expression. The activation signal-regulated (ERK1)/ERK2 (designated ERK1,2) pathway oncogenic Ras, constitutively active Raf-1, or phorbol ester...