A5035369200- Viral Infections and Outbreaks Research
- Viral Infections and Vectors
- Viral gastroenteritis research and epidemiology
- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- Disaster Response and Management
- Phagocytosis and Immune Regulation
- Neonatal Respiratory Health Research
- Respiratory Support and Mechanisms
- Neonatal Health and Biochemistry
- Immune cells in cancer
- Respiratory viral infections research
- Hepatitis B Virus Studies
- Biochemical and Molecular Research
- Mosquito-borne diseases and control
- Congenital Diaphragmatic Hernia Studies
- Bioinformatics and Genomic Networks
- Cytomegalovirus and herpesvirus research
- CRISPR and Genetic Engineering
- Sepsis Diagnosis and Treatment
- Rabies epidemiology and control
- Virology and Viral Diseases
- PARP inhibition in cancer therapy
- Immunotherapy and Immune Responses
- COVID-19 epidemiological studies
Boston University
2020-2024
University of Massachusetts Boston
2022
National Centre for Infectious Diseases
2021
Boston Medical Center
2020
National Institute of Allergy and Infectious Diseases
2018
National Institutes of Health
2018
As the COVID-19 pandemic continues to spread, investigating processes underlying interactions between SARS-CoV-2 and its hosts is of high importance. Here, we report identification CD209L/L-SIGN related protein CD209/DC-SIGN as receptors capable mediating entry into human cells. Immunofluorescence staining tissues revealed prominent expression CD209L in lung kidney epithelia endothelia. Multiple biochemical assays using a purified recombinant spike receptor-binding domain (S-RBD) or S1...
Abstract As the COVID-19 pandemic continues to spread, investigating processes underlying interactions between SARS-CoV-2 and its hosts is of high importance. Here, we report identification CD209L/L-SIGN related protein CD209/DC-SIGN as receptors capable mediating entry into human cells. Immunofluorescence staining tissues revealed prominent expression CD209L in lung kidney epithelium endothelium. Multiple biochemical assays using a purified recombinant spike receptor binding domain (S-RBD)...
Abstract Some filoviruses can be transmitted to humans by zoonotic spillover events from their natural host and filovirus outbreaks have occured with increasing frequency in the last years. The Lloviu virus (LLOV), was identified 2002 Schreiber’s bats ( Miniopterus schreibersii ) Spain subsequently detected Hungary. Here we isolate infectious LLOV blood of a live sampled bat is sequenced cultured sp. kidney cell line SuBK12-08. It furthermore able infect monkey human cells, suggesting that...
Exacerbated and persistent innate immune response marked by pro-inflammatory cytokine expression is thought to be a major driver of chronic COVID-19 pathology. Although macrophages are not the primary target cells SARS-CoV-2 infection in humans, viral RNA antigens activated monocytes have been detected post-mortem samples, dysfunctional hypothesized contribute protracted hyper-inflammatory state patients. In this study, we demonstrate that CD169, myeloid cell specific I-type lectin,...
Next generation sequencing has revealed the presence of numerous RNA viruses in animal reservoir hosts, including many closely related to known human pathogens. Despite their zoonotic potential, most these remain understudied due not yet being cultured. While reverse genetic systems can facilitate virus rescue, this is often hindered by missing viral genome ends. A prime example Lloviu (LLOV), an uncultured filovirus that highly pathogenic Ebola virus. Using minigenome systems, we...
Abstract Lloviu cuevavirus (LLOV) was the first identified member of Filoviridae family outside Ebola and Marburgvirus genera. A massive die-off Schreibers’s bats ( Miniopterus schreibersii ) in Iberian Peninsula 2002 led to its initial discovery. Recent studies with recombinant wild-type LLOV isolates confirmed zoonotic nature virus vitro. We examined bat samples from Italy for presence an area currently known distribution range virus. detected one positive sample 2020, sequenced complete...
ABSTRACT PARP14 is a 203 kDa multi-domain protein that primarily known as an ADP-ribosyltransferase, and involved in variety of cellular functions including DNA damage, microglial activation, inflammation, cancer progression. In addition, upregulated by interferon (IFN), indicating role the antiviral response. Furthermore, has evolved under positive selection, again it host-pathogen conflict. We found required for increased IFN-I production response to coronavirus infection lacking...
The most severe and fatal infections with SARS-CoV-2 result in the acute respiratory distress syndrome, a clinical phenotype of coronavirus disease 2019 (COVID-19) that is associated virions targeting epithelium distal lung, particularly facultative progenitors this tissue, alveolar epithelial type 2 cells (AT2s). Little known about initial responses human lung alveoli to infection due part inability access these from patients, at early stages disease. Here we present an vitro model...
Exacerbated and persistent innate immune response marked by pro-inflammatory cytokine expression is thought to be a major driver of chronic COVID-19 pathology. Although macrophages are not the primary target cells SARS-CoV-2 infection in humans, viral RNA antigens activated monocytes have been detected post-mortem samples, dysfunctional hypothesized contribute protracted hyper-inflammatory state patients. In this study, we demonstrate that CD169, myeloid cell specific I-type lectin,...
Liver damage and an exacerbated inflammatory response are hallmarks of Ebola virus (EBOV) infection. Little is known about the intrinsic to infection in human hepatocytes their contribution inflammation. Here, we present induced pluripotent stem cell (iPSC)-derived hepatocyte-like (HLC) platform define hepato-intrinsic EBOV We used this show robust infection, with characteristic ultrastructural changes evidence for viral replication. Transcriptomics analysis revealed a delayed minimal early...
Abstract Several molecular datasets have been recently compiled to characterize the activity of SARS-CoV-2 within human cells. Here we extend computational methods integrate several different types sequence, functional and interaction data reconstruct networks pathways activated by virus in host We identify key proteins these further intersect them with genes differentially expressed at conditions that are known impact viral activity. top ranked do not directly interact proteins....
SUMMARY The coronavirus disease 2019 (COVID-19) pandemic has created an urgent need for therapeutics that inhibit the SARS-CoV-2 virus and suppress fulminant inflammation characteristic of advanced illness. Here, we describe anti-COVID-19 potential PTC299, orally available compound is a potent inhibitor dihydroorotate dehydrogenase (DHODH), rate-limiting enzyme de novo pyrimidine biosynthesis pathway. In tissue culture, PTC299 manifests robust, dose-dependent, DHODH-dependent inhibition SARS...
Abstract Next generation sequencing has revealed the presence of many RNA viruses in animal reservoir hosts, including closely related to known human pathogens. Despite their zoonotic potential, these remain understudied due not yet being cultured. While reverse genetic systems can facilitate virus rescue, this is often hindered by missing viral genome ends. A prime example Lloviu (LLOV), an uncultured filovirus that highly pathogenic Ebola virus. Using minigenome systems, we complemented...
Abstract Lloviu cuevavirus (LLOV) was the first identified member of Filoviridae family outside Ebola and Marburgvirus genera. A massive die-off Schreibers’ bent-winged bats ( Miniopterus schreibersii ) in Iberian Peninsula 2002 led to its discovery. Studies with recombinant wild-type LLOV isolates confirmed susceptibility human-derived cell lines primary human macrophages infection vitro . Based on these data, is now considered as a potential zoonotic virus unknown pathogenicity humans...
Abstract Liver damage and an exacerbated inflammatory response are hallmarks of Ebola virus (EBOV) infection. Little is known about the intrinsic to infection in human hepatocytes their contribution observed response. Here, we present iPSC-derived hepatocyte platform define hepato-intrinsic EBOV Transcriptomics analysis revealed a delayed host with minimal transcriptomic changes at one day post (dpi) followed by general downregulation genes associated hepatic functions upregulation...