A5010332288- Clinical Laboratory Practices and Quality Control
- SARS-CoV-2 and COVID-19 Research
- Electronic Health Records Systems
- Meta-analysis and systematic reviews
- COVID-19 Clinical Research Studies
- Biomedical Text Mining and Ontologies
- Healthcare cost, quality, practices
- Health Sciences Research and Education
- Clinical Reasoning and Diagnostic Skills
- SARS-CoV-2 detection and testing
- Immune Cell Function and Interaction
- Liver Disease Diagnosis and Treatment
- Healthcare Technology and Patient Monitoring
- COVID-19 and healthcare impacts
- Sepsis Diagnosis and Treatment
- Immune Response and Inflammation
- Immunodeficiency and Autoimmune Disorders
- Patient Safety and Medication Errors
- T-cell and B-cell Immunology
- Quality and Safety in Healthcare
- Biosensors and Analytical Detection
- Cytokine Signaling Pathways and Interactions
- AI in cancer detection
- Medical Malpractice and Liability Issues
- interferon and immune responses
Harvard University
2015-2025
Mass General Brigham
2018-2025
Yale Cancer Center
2025
Massachusetts General Hospital
2015-2024
Political Research Associates
2023
Koç University
2022
Marmara University
2022
University of Pittsburgh Medical Center
2022
Roche (Switzerland)
2022
Michigan Medicine
2022
This study demonstrates that endogenously produced interferon gamma (IFN-gamma) forms the basis of a tumor surveillance system controls development both chemically induced and spontaneously arising tumors in mice. Compared with wild-type mice, mice lacking sensitivity to either IFN-gamma (i.e., receptor-deficient mice) or all IFN family members Stat1-deficient developed more rapidly greater frequency when challenged different doses chemical carcinogen methylcholanthrene. In addition,...
The developmental commitment to a T helper 1 (Th1)- or Th2-type response can significantly influence host immunity pathogens. Extinction of the IL-12 signaling pathway during early Th2 development provides mechanism that allows stable phenotype commitment. In this report we demonstrate extinction in cells results from selective loss receptor (IL-12R) beta 2 subunit expression. To determine basis for loss, examined IL-12R expression Th cell treatment with different cytokines. is not expressed...
The diagnosis of COVID-19 requires integration clinical and laboratory data. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) diagnostic assays play a central role in have fixed technical performance metrics. Interpretation becomes challenging because the sensitivity changes as virus clears immune response emerges. Our goal was to examine two most common SARS-CoV-2 test modalities, polymerase chain reaction (PCR) serology, over disease course provide insight into their...
Many studies have examined the impact of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants on neutralizing antibody activity after they become dominant strains. Here, we evaluate consequences further viral evolution. We demonstrate mechanisms through which SARS-CoV-2 receptor binding domain (RBD) can tolerate large numbers simultaneous escape mutations and show that pseudotypes containing up to seven mutations, as opposed one three found in previously studied concern, are...
Interferon gamma (IFN-gamma) responsiveness in certain cells depends on the state of cellular differentiation or activation. Here an vitro developmental system was used to show that IFN-gamma produced during generation CD4+ T helper cell type 1 (TH1) subset extinguishes expression receptor beta subunit, resulting TH1 are unresponsive IFN-gamma. This chain loss also occurred IFN-gamma-treated TH2 and thus represents a specific response rather than TH1-specific event. These results define...
While clinical laboratories report most test results as individual numbers, findings, or observations, diagnosis usually relies on the of multiple tests. Clinical decision support that integrates elements laboratory data could be highly useful in enhancing diagnosis.Using analyte ferritin a proof concept, we extracted from patient testing and applied variety machine-learning algorithms to predict using other We compared predicted with measured reviewed selected cases assess value ferritin.We...
We define the scope and needs within new discipline of computational pathology, a critical to future both practice pathology and, more broadly, medical in general.To pathology.A meeting was convened Boston, Massachusetts, July 2014 prior annual Association Pathology Chairs meeting, it attended by variety pathologists, including individuals highly invested informatics as well chairs departments.The made recommendations promote clearly defining field articulating its value propositions;...
A key challenge in clinical data mining is that most datasets contain missing data. Since many commonly used machine learning algorithms require complete (no data), analytic approaches often entail an imputation procedure to "fill in" However, although a temporal component, methods do not adequately accommodate longitudinal time-based We sought develop new algorithm, 3-dimensional multiple with chained equations (3D-MICE), can perform accurate of time series data.We extracted laboratory test...
The immunogenicity and reactogenicity of SARS-CoV-2 vaccines in patients with cancer are poorly understood.We performed a prospective cohort study adults solid-organ or hematologic cancers to evaluate anti-SARS-CoV-2 immunoglobulin A/M/G spike antibodies, neutralization, ≥ 7 days following two doses mRNA-1273, BNT162b2, one dose Ad26.COV2.S. We analyzed responses by multivariate regression included data from 1,638 healthy controls, previously reported, for comparison.Between April July 2021,...
Management of laboratory test utilization presents an ongoing challenge. Most studies reported in the literature have described efforts to control one or a few tests, but results cannot be generalized broader management strategy. Herein we report our experiences with organizational program during 10-year period. Cumulatively, has achieved significant success, saving millions dollars blood components and reducing inpatient tests per discharge by 26%. Highlights from include importance...
Sensitive and specific severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) serologic assays are needed to inform diagnostic, therapeutic, public health decision-making. We evaluated three commercial as stand-alone tests components of two-test algorithms. Two nucleocapsid antibody (Abbott IgG Roche total antibody) one spike protein test (DiaSorin IgG) were included. assessed sensitivity using 128 serum samples from symptomatic PCR-confirmed disease 2019 (COVID-19)-infected patients...
Complex coagulation test panels ordered by clinicians are typically reported to without a patient-specific interpretive paragraph.To survey regarding pathologist-generated interpretations of complex laboratory testing and assess the ability educate orderers.Surveys were conducted physicians ordering that included narrative interpretation. Evaluation order requisitions was performed interpretation's influence on practices.Physicians at large academic medical center hospital in Boston, Mass,...
Reporting of laboratory critical values has become an issue national attention as illustrated by recent guidelines described in the National Patient Safety Goals Joint Commission on Accreditation Healthcare Organizations. Herein, we report results analysis 37,503 consecutive at our institution, a large urban academic medical center. We evaluated value reporting test, specialty, patient type, clinical care area, time day, and limits. Factors leading to delays are identified, describe...
Clinicians have traditionally ordered laboratory tests using paper-based orders and requisitions. However, paper are becoming increasingly incompatible with the complexities, challenges, resource constraints of our modern healthcare systems being replaced by electronic order entry systems. Electronic that allow direct provider input diagnostic testing or medication into a computer system known as Computerized Provider Order Entry (CPOE) Adoption CPOE may offer institutions many benefits,...