A5015136634- Cancer Immunotherapy and Biomarkers
- Monoclonal and Polyclonal Antibodies Research
- HIV Research and Treatment
- Peptidase Inhibition and Analysis
- Liver Disease Diagnosis and Treatment
- Hepatitis C virus research
- HIV/AIDS drug development and treatment
- HIV-related health complications and treatments
- Immunotherapy and Immune Responses
- HIV/AIDS Research and Interventions
- SARS-CoV-2 and COVID-19 Research
- Liver Disease and Transplantation
- Hepatitis B Virus Studies
- COVID-19 Clinical Research Studies
- Immune Cell Function and Interaction
- Galectins and Cancer Biology
- T-cell and B-cell Immunology
- Diabetes and associated disorders
- Vitamin D Research Studies
- Dendrimers and Hyperbranched Polymers
- COVID-19 epidemiological studies
- Reproductive System and Pregnancy
- Advanced Breast Cancer Therapies
- Pneumocystis jirovecii pneumonia detection and treatment
- Diabetes, Cardiovascular Risks, and Lipoproteins
Agenus (United States)
2022-2024
Ragon Institute of MGH, MIT and Harvard
2017-2022
Brigham and Women's Hospital
2022
Instituto de Salud Carlos III
2013-2021
Centro Nacional de Microbiologia
2014-2021
Harvard University
2020
Hospital General Universitario Gregorio Marañón
2016-2019
Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine
2017-2019
The relationship between SARS-CoV-2 viral load and risk of disease progression remains largely undefined in coronavirus 2019 (COVID-19). Here, we quantify from participants with a diverse range COVID-19 severity, including those requiring hospitalization, outpatients mild disease, individuals resolved infection. We detected plasma RNA 27% hospitalized participants, 13% diagnosed COVID-19. Amongst the COVID-19, report that higher prevalence detectable is associated worse respiratory lower...
Antiretroviral treatment initiation immediately after birth reduces HIV-1 viral reservoir size and alters innate immune responses in neonates.
Abstract Conventional immune checkpoint inhibitors (ICI) targeting cytotoxic T-lymphocyte antigen 4 (CTLA-4) elicit durable survival but primarily in patients with immune-inflamed tumors. Although the mechanisms underlying response to anti–CTLA-4 remain poorly understood, Fc-gamma receptor (FcγR) IIIA coengagement seems critical for activity, potentially explaining modest clinical benefits of approved antibodies. We demonstrate that engineered enhanced FcγR affinity leverages FcγR-dependent...
Despite low plasma human immunodeficiency virus (HIV) RNA, HIV controllers have evidence of viral replication and elevated inflammation. We assessed the effect antiretroviral therapy (ART) on suppression, immune activation, quality life (QoL).A5308 was a prospective, open-label study rilpivirine/emtricitabine/tenofovir disoproxil fumarate in ART-naive (N = 35), defined as having RNA <500 copies/mL for ≥12 months. The primary outcome measured change %CD38+HLA-DR+ CD8+ T cells. Residual...
Highlights•ART initiation in neonates leads to a profound decline of intact HIV-1 proviruses•The longitudinal proviruses is linked NK cell responses•Initiation ART corrects innate immune perturbations ILCs and DCs•ILCs, macrophages, DCs are not associated with provirusesSummaryInitiation antiretroviral therapy (ART) infected within hours after birth limits viral reservoir seeding but does prevent long-term persistence. Here, we report parallel assessments cells antiviral responses unique...
Anionic carbosilane dendrons decorated with sulfonate functions and one thiol moiety at the focal point have been used to synthesize water-soluble gold nanoparticles (AuNPs) through direct reaction of dendrons, precursor, reducing agent in water, also a place-exchange reaction. These characterized by NMR spectroscopy, TEM, thermogravimetric analysis, X-ray photoelectron spectroscopy (XPS), UV/Vis elemental zeta-potential measurements. The interacting ability anionic was investigated EPR...
Objectives: Immune dysregulation is a hallmark of HIV and hepatitis C virus (HCV) infections. We aimed to evaluate the relationship between liver stiffness measurement (LSM) biomarkers T-cell activation, bacterial translocation, inflammation, endothelial dysfunction, coagulopathy in HIV/HCV-coinfected patients. Design: Cross-sectional study. Methods: studied 238 patients, 32 healthy controls, 39 HIV-monoinfected Patients were stratified according LSM into four groups: less than 12.5,...
Humoral responses in COVID-19 disease are often of limited durability, as seen with other human coronavirus epidemics. To address the underlying etiology, we examined postmortem thoracic lymph nodes and spleens acute SARS-CoV-2 infection observed absence germinal centers, a striking reduction Bcl-6+ center B cells but preservation AID+ cells. Absence centers correlated an early specific block Bcl-6+TFH cell differentiation together increase T-bet+TH1 aberrant extra-follicular TNF-a...
Interferon alpha (IFN-α) can potently reduce human immunodeficiency virus type 1 (HIV-1) replication in tissue culture and animal models, but may also modulate residual viral reservoirs that persist despite suppressive antiretroviral combination therapy. However, mechanisms leading to reservoir reduction during IFN-α treatment are unclear.We analyzed HIV-1 gag DNA levels CD4 T cells by digital droplet polymerase chain reaction CD8 T-cell natural killer (NK) cell phenotypes flow cytometry a...
Unprotected heterosexual intercourse is the first route for sustaining global spread of human immunodeficiency virus type 1 (HIV-1), being responsible 80% new HIV-1 infections in world.
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) kinetics remain understudied, including the impact of remdesivir. In hospitalized individuals, peak sputum viral load occurred in week symptoms, whereas viremia peaked within 1 symptom-onset, suggesting early systemic seeding SARS-CoV-2. Remdesivir treatment was associated with faster decay.
Background: Eradication of hepatitis C virus (HCV) promotes an improvement in liver disease and the deactivation immune system. Here, we aimed to evaluate changes scores plasma biomarkers following HCV clearance with direct-acting antivirals (DAAs) HIV-infected patients advanced HCV-related cirrhosis. Methods: We performed observational study 50 cirrhosis who received DAAs therapy. Variables were assessed at baseline 48 weeks after treatment completion. Epidemiological clinical data...
The impact of very early infant treatment on human immunodeficiency virus (HIV) reservoir, and markers for success, require study.The Early Infant Treatment Study (EIT) enrolled 40 children living with HIV started antiretroviral (ART) at <7 days age, 23 who had between 30-365 to serve as controls. Quantitative DNA was evaluated every 1-3 months in peripheral blood mononuclear cells. 84-week repeat qualitative whole polymerase chain reaction dual enzyme immunosorbent assay were...
Background: Advanced cirrhosis is related to alterations in immunity. We aimed evaluate the levels of peripheral CD4+ T cells (Tregs) and plasma cytokine patients coinfected with human immunodeficiency virus hepatitis C (HIV/HCV) according liver fibrosis stages [evaluated as stiffness measure (LSM)] their linear relationship. Methods: performed a cross-sectional study on 238 HIV/HCV-coinfected (119 had <12.5 kPa, 73 12.5–25 46 >25 kPa). Peripheral T-cell subsets were phenotyped by flow...
The IL7RA polymorphisms have recently been associated with CD4+ T-cell decline in untreated HIV-infected subjects and recovery patients on combination antiretroviral therapy (cART). aim of this study was to evaluate whether are long-term cART.We performed a retrospective 151 naïve cART severe immunodeficiency (CD4+ counts ≤200 cells/mm(3) ). polymorphisms' genotyping using Sequenom's MassARRAY platform. outcome variable the time achieve first value count ≥500 during follow-up.Two different...
Abstract Background The adiponectin ( ADIPOQ ) rs2241766 polymorphism is related to metabolic abnormalities. aim of this study was evaluate the association with serum dyslipidemia and insulin resistance IR in human immunodeficiency virus HIV )/hepatitis C HCV )‐coinfected patients. Methods We carried out a cross‐sectional on 262 polymorphisms were genotyped by GoldenGate ® assay. Generalized linear models GLMs used compare continuous outcome variables (total cholesterol TC ), triglycerides...
Interleukin-7 (IL-7) is a critical factor for T cell development and maintaining restoring homeostasis of mature cells. Polymorphisms at α-chain the IL-7 receptor (IL7R or CD127) gene are related to evolution HIV-infection, but there no data concerning hepatitis C virus (HCV) infection. The aim this study was analyze association between IL7R polymorphisms severe liver disease in HCV/HIV coinfected patients. We performed cross-sectional 220 naïve patients who underwent biopsy. (rs6897932,...
The Fat Mass and Obesity-Associated Protein (FTO) gene rs9939609 single nucleotide polymorphism (SNP) has been associated with obesity, metabolic syndrome, insulin resistance (IR), type 2 diabetes mellitus in the general population. aim of our study was to examine for first time association disturbances, liver disease virologic response hepatitis C virus (HCV) therapy pegylated-interferon-alpha plus ribavirin (pegIFNα/RBV) human immunodeficiency (HIV)/HCV coinfected patients. We carried out...
Abstract We aimed to evaluate the association of plasma biomarkers linked inflammation (bacterial translocation, inflammatory response, and endothelial dysfunction), coagulopathy, angiogenesis with severity liver cirrhosis (assessed by Child-Pugh-Turcotte score, CTP) Child-Pugh B (CTP 7–9) in patients advanced hepatitis C virus (HCV)-related cirrhosis. carried out a cross-sectional study 97 HCV-related (32 HCV-monoinfected 65 HIV/HCV-coinfected). Plasma were measured ProcartaPlex multiplex...
To analyze the relationship of SLC30A8 rs13266634 polymorphism with insulin resistance and dyslipidemia in HIV/hepatitis C virus (HCV)-coinfected patients.Cross-sectional study 260 HIV/HVC-coinfected patients.SLC30A8 polymorphisms were genotyped by GoldenGate assay. Genetic data analyzed under dominant inheritance model (CT/TT versus CC). Cholesterol, triglycerides, high-density lipoprotein (HDL-C), low-density (LDL-C), LDL-C/HDL-C, atherogenic index, homeostatic assessment (HOMA-IR) values...
BackgroundThe persistence of HIV-1-infected cells during antiretroviral therapy is well documented but may be modulated by early initiation in infants.MethodsHere, we longitudinally analyzed the proviral landscape nine infants with vertical HIV-1 infection from Mozambique over a median period 24 months, using single-genome, near full-length, next-generation sequencing.ResultsWe observed rapid decline frequency intact proviruses, leading to disproportional under-representation sequences...