A5079125099- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- Enzyme Production and Characterization
- Xenotransplantation and immune response
- Glycosylation and Glycoproteins Research
- Erythrocyte Function and Pathophysiology
- Carbohydrate Chemistry and Synthesis
- T-cell and B-cell Immunology
- Monoclonal and Polyclonal Antibodies Research
- Peptidase Inhibition and Analysis
- Immunotherapy and Immune Responses
- Blood groups and transfusion
- Neonatal Health and Biochemistry
- Advanced biosensing and bioanalysis techniques
- RNA and protein synthesis mechanisms
- SARS-CoV-2 detection and testing
- Transgenic Plants and Applications
- Polyamine Metabolism and Applications
- Viral gastroenteritis research and epidemiology
- vaccines and immunoinformatics approaches
- Lysosomal Storage Disorders Research
- Coffee research and impacts
- Respiratory viral infections research
- COVID-19 epidemiological studies
- Studies on Chitinases and Chitosanases
Hangzhou Children's Hospital
2025
Nantong University
2025
Moderna Therapeutics (United States)
2024
University of Duisburg-Essen
2021-2023
Phillips Academy
2022-2023
University of Alberta
2023
Baylor Scott & White Health
2023
Ragon Institute of MGH, MIT and Harvard
2020-2022
Brigham and Women's Hospital
2021
Harvard University
2020-2021
The first and major clinical obstacle in xenotransplantation is antibody-mediated hyperacute rejection. Although human natural antibodies against Galalpha1,3Gal (Gal) antigens, which are common on porcine cells organs, have been identified to play a role rejection, other non-Gal epitopes may be also involved the process. Here, we present evidence suggesting that majority of anti-non-Gal specific for carbohydrate structures carrying terminally linked N-glycolylneuraminic acid (NeuGc),...
Abstract Antibodies serve as biomarkers of infection, but if sustained can confer long-term immunity. Yet, for most clinically approved vaccines, binding antibody titers only a surrogate protection. Instead, the ability vaccine induced antibodies to neutralize or mediate Fc-effector functions is mechanistically linked While evidence has begun point persisting responses among SARS-CoV-2 infected individuals, cases re-infection have emerge, calling protective nature humoral immunity against...
Vaccination of nonhuman primates with SARS-CoV-2 spike trimers and AS03 adjuvant induces neutralizing antibodies sufficient to protect from disease.
ABSTRACT mRNA-1647 is an investigational mRNA-based vaccine against cytomegalovirus (CMV) that contains sequences encoding the CMV proteins glycoprotein B and pentamer. Humoral cellular immune responses were evaluated in blood samples collected from healthy CMV-seropositive CMV-seronegative adults who participated a phase 1 trial of three-dose series (NCT03382405). Neutralizing antibody (nAb) titers fibroblast epithelial cell infection sera recipients higher than those control remained...
Abstract Adjuvanted soluble protein vaccines have been used extensively in humans for protection against various viral infections based on their robust induction of antibody responses. Here, prefusion-stabilized spike trimers (preS dTM) from the severe acute respiratory syndrome coronavirus (SARS-CoV-2) were formulated with adjuvant AS03 and administered twice to nonhuman primates (NHP). Binding functional neutralization assays systems serology revealed that NHP developed AS03-dependent...
Abstract The mechanisms by which antibodies confer protection vary across vaccines, ranging from simple neutralization to functions requiring innate immune recruitment via Fc-dependent mechanisms. role of adjuvants in shaping the maturation antibody-effector remains under investigated. Using systems serology, we compared licensed vaccines (AS01 B /AS01 E /AS03/AS04/Alum) combined with a model antigen. Antigen-naive adults received two adjuvanted immunizations followed late revaccination...
Humoral responses in COVID-19 disease are often of limited durability, as seen with other human coronavirus epidemics. To address the underlying etiology, we examined postmortem thoracic lymph nodes and spleens acute SARS-CoV-2 infection observed absence germinal centers, a striking reduction Bcl-6+ center B cells but preservation AID+ cells. Absence centers correlated an early specific block Bcl-6+TFH cell differentiation together increase T-bet+TH1 aberrant extra-follicular TNF-a...
Abstract Recently approved vaccines have already shown remarkable protection in limiting SARS-CoV-2 associated disease. However, immunologic mechanism(s) of protection, as well how boosting alters immunity to wildtype and newly emerging strains, remain incompletely understood. Here we deeply profiled the humoral immune response a cohort non-human primates immunized with stable recombinant full-length spike (S) glycoprotein (NVX-CoV2373) at two dose levels, administered single or two-dose...
This research was conducted to establish a fast-track detection scheme for the adenovirus, common respiratory pathogen in children, facilitate precise clinical diagnosis and treatment.
B cell receptors (BCRs) display a combination of variable (V)-gene-encoded complementarity determining regions (CDRs) and adaptive/hypervariable CDR3 loops to engage antigens. It has long been proposed that the former tune for recognition pathogens or groups pathogens. To experimentally evaluate this within human antibody repertoire, we perform immune challenges in transgenic mice bear diverse light chains but are constrained different VH-genes. We find that, six commonly deployed VH...
Abstract The development of a portfolio SARS-CoV-2 vaccines to vaccinate the global population remains an urgent public health imperative. Here, we demonstrate capacity subunit vaccine under clinical development, comprising Spike protein receptor binding domain displayed on two-component nanoparticle (RBD-NP), stimulate robust and durable neutralizing antibody (nAb) responses protection against in non-human primates. We evaluated five different adjuvants combined with RBD-NP including Essai...
BACKGROUND : It has previously been shown that 1 and 2 units (200 – 400 mL) of red cells (RBCs) enzymatically converted from group B to O by treatment with alpha‐galactosidase (ECO RBCs) are safe efficacious when transfused normal or A persons. STUDY DESIGN AND METHODS The current report describes studies in which 1) subjects received large volumes these (3 units), 2) some underwent transfusion several months later, 3) ECO RBCs were prepared the use recombinant coffee bean a subject,...
To provide information on the specificity of induced anti-pig antibodies (Abs) in baboons after exposure and sensitization to pig antigens.Baboons (n=7) received either porcine mobilized peripheral blood progenitor cells (n=3), kidney (n=3) or heart (n=1) transplants. After rejection these organs, pre- post-rejection sera were analyzed by enzyme-linked immunosorbent assay (ELISA) flow cytometry detect measure anti-Galactosealpha1,3Galactose (Gal) anti-non-Gal Abs. study carbohydrate...
Obesity is a key correlate of severe SARS-CoV-2 outcomes while the role obesity on risk infection, symptom phenotype, and immune response remain poorly defined. We examined data from prospective cohort study to address these questions. Serostatus, body mass index, demographics, comorbidities, prior COVID-19 compatible symptoms were assessed at baseline serostatus monthly thereafter. immunoassays included an IgG ELISA targeting spike RBD, multiarray Luminex 20 viral antigens, pseudovirus...
Background. Xenotransplantation is considered one of possible solutions for the serious shortage organs and cells in transplantation. Although αGal epitope (Galα1,3Galβ1,4GlcNAc-R) has been identified as being a major xenoantigen responsible hyperacute rejection, removal anti-αGal antibody alone from human serum insufficient to circumvent antibody-mediated immune responses. Methods Results. We report here characterization xenoreactive natural antibodies against antigens without (nonαGal...