A5044053330- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- Viral Infections and Immunology Research
- Mosquito-borne diseases and control
- Animal Virus Infections Studies
- Viral Infections and Vectors
- RNA Research and Splicing
- vaccines and immunoinformatics approaches
- Electrochemical Analysis and Applications
- Electrochemical sensors and biosensors
- Polyomavirus and related diseases
- Respiratory viral infections research
- Monoclonal and Polyclonal Antibodies Research
- interferon and immune responses
- Liver Disease Diagnosis and Treatment
- Conducting polymers and applications
- Hepatitis B Virus Studies
- Virology and Viral Diseases
- Hepatitis C virus research
- RNA and protein synthesis mechanisms
- Cytomegalovirus and herpesvirus research
- Free Radicals and Antioxidants
- SARS-CoV-2 detection and testing
- Immunotherapy and Immune Responses
- Virus-based gene therapy research
Novavax (United States)
2020-2023
National Institute of Allergy and Infectious Diseases
2018-2023
Global Viral
2022-2023
National Institutes of Health
2020-2021
University of Maryland, College Park
2021
University of California, Irvine
2013-2018
Utrecht University
2013
Radboud University Nijmegen
2013
Leiden University
2013
San Diego State University
2009-2011
Abstract The COVID-19 pandemic continues to spread throughout the world with an urgent need for a safe and protective vaccine effectuate herd protection control of SARS-CoV-2. Here, we report development SARS-CoV-2 subunit (NVX-CoV2373) from full-length spike (S) protein that is stable in prefusion conformation. NVX-CoV2373 S form 27.2-nm nanoparticles are thermostable bind high affinity human angiotensin-converting enzyme 2 (hACE2) receptor. In mice, low-dose saponin-based Matrix-M adjuvant...
There is an urgent need for a safe and protective vaccine to control the global spread of SARS-CoV-2 prevent COVID-19. Here, we report immunogenicity efficacy subunit (NVX-CoV2373) produced from full-length spike (S) glycoprotein stabilized in prefusion conformation. Cynomolgus macaques (Macaca fascicularis) immunized with NVX-CoV2373 saponin-based Matrix-M™ adjuvant induced anti-S antibody that was neutralizing blocked binding human angiotensin-converting enzyme 2 (hACE2) receptor....
To disseminate through the body, Zika virus (ZIKV) is thought to exploit mobility of myeloid cells, in particular monocytes and dendritic cells. However, timing mechanisms underlying shuttling by immune cells remains unclear. understand early steps ZIKV transit from skin, at different time points, we spatially mapped infection lymph nodes (LNs), an intermediary site en route blood. Contrary prevailing hypotheses, migratory are not required for reach LNs or Instead, rapidly infects a subset...
Abstract The COVID-19 pandemic continues to spread throughout the world with an urgent need for a safe and protective vaccine effectuate herd immunity control of SARS-CoV-2. Here, we report development SARS-CoV-2 subunit (NVX-CoV2373) produced from full-length spike (S) protein, stabilized in prefusion conformation. Purified NVX-CoV2373 S form 27.2nm nanoparticles that are thermostable bind high affinity human angiotensin-converting enzyme 2 (hACE2) receptor. In mice baboons, low-dose...
ABSTRACT Coxsackieviruses are significant human pathogens, and the neonatal central nervous system (CNS) is a major target for infection. Despite extreme susceptibility of newborn infants to coxsackievirus infection viral tropism CNS, few studies have been aimed at determining long-term consequences on developing CNS. We previously described mouse model B3 (CVB3) determined that proliferating stem cells in CNS were preferentially targeted. Here, we describe later stages infection, ensuing...
The emergence of Zika virus (ZIKV) in the Americas stimulated development multiple ZIKV vaccine candidates. We previously developed two related DNA candidates encoding structural proteins that were immunogenic animal models and humans. sought to identify neutralizing antibody (NAb) properties induced by each correlated with protection nonhuman primates (NHPs). Despite eliciting equivalent NAb titers NHPs, these vaccines not equally protective. transfer vaccine-elicited into AG129 mice also...
Abstract Recently approved vaccines have already shown remarkable protection in limiting SARS-CoV-2 associated disease. However, immunologic mechanism(s) of protection, as well how boosting alters immunity to wildtype and newly emerging strains, remain incompletely understood. Here we deeply profiled the humoral immune response a cohort non-human primates immunized with stable recombinant full-length spike (S) glycoprotein (NVX-CoV2373) at two dose levels, administered single or two-dose...
SARS-CoV-2 variants have emerged with elevated transmission and a higher risk of infection for vaccinated individuals. We demonstrate that recombinant prefusion-stabilized spike (rS) protein vaccine based on Beta/B.1.351 (rS-Beta) produces robust anamnestic response in baboons against when given as booster one year after immunization NVX-CoV2373. Additionally, rS-Beta is highly immunogenic mice neutralizing antibodies WA1/2020, Beta/B.1.351, Omicron/BA.1. Mice two doses Novavax prototype...
SUMMARY Background Emerging SARS-CoV-2 variants and evidence of waning vaccine efficacy present significant obstacles toward controlling the COVID-19 pandemic. Booster doses vaccines may address these concerns by both amplifying broadening immune responses seen with initial vaccination regimens. Methods In a phase 2 study, single booster dose recombinant spike protein Matrix-M™ adjuvant (NVX-CoV2373) was administered to healthy adult participants 18 84 years age approximately 6 months...
ABSTRACT There is an urgent need for a safe and protective vaccine to control the global spread of SARS-CoV-2 prevent COVID-19. Here, we report immunogenicity efficacy subunit (NVX-CoV2373) produced from full-length spike (S) glycoprotein stabilized in prefusion conformation. Cynomolgus macaques ( Macaca fascicularis ) immunized with NVX-CoV2373 saponin-based Matrix-M adjuvant induced anti-S antibody that was neutralizing blocked binding human angiotensin-converting enzyme 2 (hACE2)...
Coxsackieviruses are significant human pathogens causing myocarditis, meningitis, and encephalitis. We previously demonstrated the ability of coxsackievirus B3 (CVB3) to persist within neonatal central nervous system (CNS) target neural stem cells. Given that CVB3 is a cytolytic virus may therefore damage cells, we characterized potential reduction in neurogenesis developing brain subsequent developmental defects occurred after loss these essential Neonatal mice were inoculated with...
Abstract Picornaviruses constitute a large group of viruses comprising medically and economically important pathogens such as poliovirus, coxsackievirus, rhinovirus, enterovirus 71 foot-and-mouth disease virus. A unique characteristic these is the use viral peptide (VPg) primer for RNA synthesis. As consequence, all newly formed molecules possess covalently linked VPg peptide. It known that enzymatically released from incoming by host protein, called TDP2, but it still unclear whether...
Abstract The COVID-19 pandemic continues to spread throughout the world with an urgent need for a safe and protective vaccine effectuate herd immunity control of SARS-CoV-2. Here, we report development SARS-CoV-2 subunit (NVX-CoV2373) produced from full-length spike (S) protein, stabilized in prefusion conformation. Purified NVX-CoV2373 S form 27.2 nm nanoparticles that are thermostable bind high affinity human angiotensin-converting enzyme 2 (hACE2) receptor. In mice baboons, low-dose...
Abstract The 2019 outbreak of a severe respiratory disease caused by an emerging coronavirus, SARS-CoV-2, has spread globally with high morbidity and mortality. Co-circulating seasonal influenza greatly diminished recently, but expected to return novel strains emerging, thus requiring annual strain adjustments. We have developed recombinant hemagglutinin (HA) quadrivalent nanoparticle vaccine (qNIV) produced using established insect cell expression system produce nanoparticles. Influenza...
The voltammetry of 2,3,5,6-tetramethyl-p-phenylenediamine, H2PD, has been studied and compared to that its isomer N,N,N'N'-tetramethyl-p-phenylenediamine, Me2PD. Both undergo two reversible electron transfer processes in acetonitrile nominally correspond 1e- oxidation the radical cations, Me2PD+ H2PD+, a second at more positive potentials quinonediimine dications, Me2PD2+ H2PD2+. While Me2PD agrees with this simple mechanism, H2PD does not. voltammetric wave is too small. UV/Vis...
ABSTRACT Viruses of the Enterovirus genus picornaviruses, including poliovirus, coxsackievirus B3 (CVB3), and human rhinovirus, commandeer functions host cell proteins to aid in replication their small viral genomic RNAs during infection. One these is a cellular DNA repair enzyme known as 5′ tyrosyl-DNA phosphodiesterase 2 (TDP2). TDP2 was previously demonstrated mediate cleavage unique covalent linkage between protein (VPg) end picornavirus RNAs. Although VPg absent from actively...
Abstract Recently approved vaccines have already shown remarkable protection in limiting SARS-CoV-2 associated disease. However, immunologic mechanism(s) of protection, as well how boosting alters immunity to wildtype and newly emerging strains, remain incompletely understood. Here we deeply profiled the humoral immune response a cohort non-human primates immunized with stable recombinant full-length spike (S) glycoprotein (NVX-CoV2373) at two dose levels, administered single or two-dose...
Abstract Zika virus (ZIKV) deoxyribonucleic acid vaccine VRC5283 encoding viral structural genes has been shown to be immunogenic in humans. Recognizing that antigenically related flaviviruses cocirculate regions with ZIKV activity, we explored the degree of antibody cross-reactivity elicited by this candidate using genetically diverse flaviviruses. The response vaccinated individuals no evidence prior flavivirus infection or experience had a limited capacity bind heterologous viruses. In...
Abstract The severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) continues to spread globally. As SARS-CoV-2 has transmitted from person person, variant viruses have emerged with elevated transmission rates and higher risk of infection for vaccinees. We present data showing that a recombinant prefusion-stabilized Spike (rS) protein based on the B.1.351 sequence (rS-B.1.351) was highly immunogenic in mice produced neutralizing antibodies against SARS-CoV-2/WA1, B.1.1.7, B.1.351. Mice...
Abstract Transplantation of major histocompatibility complex-mismatched mouse neural precursor cells (NPCs) into mice persistently infected with the neurotropic JHM strain hepatitis virus (JHMV) results in rapid rejection that is mediated, part, by T cells. However, contribution innate immune response to allograft a model viral-induced neurological disease has not been well defined. Herein, we demonstrate natural killer (NK) cell-expressing-activating receptor NKG2D participates transplanted...