A5033151388- HIV Research and Treatment
- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- T-cell and B-cell Immunology
- Cytomegalovirus and herpesvirus research
- SARS-CoV-2 and COVID-19 Research
- Herpesvirus Infections and Treatments
- Monoclonal and Polyclonal Antibodies Research
- Animal Virus Infections Studies
- COVID-19 Clinical Research Studies
- Glycosylation and Glycoproteins Research
- SARS-CoV-2 detection and testing
- HIV-related health complications and treatments
- Multiple Myeloma Research and Treatments
- Immune Response and Inflammation
- Inflammasome and immune disorders
- Virus-based gene therapy research
- Bone health and treatments
- CAR-T cell therapy research
- interferon and immune responses
- Long-Term Effects of COVID-19
- Viral Infections and Immunology Research
- Malaria Research and Control
- Reproductive System and Pregnancy
- Virology and Viral Diseases
University of Louisiana at Lafayette
2017-2025
Emory University
2007-2021
Emory and Henry College
2021
Division of Parasitic Diseases and Malaria
2016
Center for Global Health
2016
Emory National Primate Research Center
2012-2013
Georgia State University
2004-2011
Teer Coatings (United Kingdom)
2007-2009
Winship Cancer Institute
2007
Fred Hutch Cancer Center
1991
HIV/SIV infections induce chronic immune activation with remodeling of lymphoid architecture and hypergammaglobulinemia, although the mechanisms leading to such symptoms remain be fully elucidated. Moreover, lymph nodes have been highlighted as a predilection site for SIV escape in vivo. Following 20 rhesus macaques infected SIVmac239 they progress from pre-infection acute infection, we document first time, our knowledge, local dynamics T follicular helper (T(FH)) cells B situ. Progression...
Despite successful control of viremia, many HIV-infected individuals given antiretroviral therapy (ART) exhibit residual inflammation, which is associated with non-AIDS-related morbidity and mortality may contribute to virus persistence during ART. Here, we investigated the effects IL-21 administration on both inflammation in ART-treated, SIV-infected rhesus macaques (RMs). Compared animals only ART, RMs ART showed improved restoration intestinal Th17 Th22 cells a more effective reduction...
Antiretroviral therapy (ART) paradoxically intensifies bone loss in the setting of HIV infection. Although extent varies, it occurs with virtually all ART types, suggesting a common pathway that may be aligned disease reversal. Using an animal model immunodeficiency we recently demonstrated immune activation associated CD4 T-cell reconstitution induces increased production osteoclastogenic cytokines RANKL and TNFα by cells, driving enhanced resorption mineral density.To confirm these...
Despite the remarkable efficacy of COVID-19 vaccines, waning immunity and emergence SARS-CoV-2 variants such as Omicron represents a global health challenge. Here, we present data from study in nonhuman primates demonstrating durable protection against BA.1 variant induced by subunit vaccine comprising receptor binding domain ancestral strain (RBD-Wu) on I53-50 nanoparticle adjuvanted with AS03, which was recently authorized for use individuals 18 years or older. Vaccination neutralizing...
In pathogenic HIV and SIV infections of humans rhesus macaques (RMs), preferential depletion CD4+ Th17 cells correlates with mucosal immune dysfunction disease progression. Interleukin (IL)-21 promotes differentiation cells, long-term maintenance functional CD8+ T memory B antibody-secreting plasma cells. We hypothesized that administration IL-21 will improve function in the context HIV/SIV infections. To test this hypothesis, we infected 12 RMs SIVmac239 at day 14 post-infection treated six...
Adolescents are a growing population of people living with HIV. The period between weaning and sexual debut presents low-risk window for HIV acquisition, making early childhood an ideal time implementing immunization regimen. Because the elicitation broadly neutralizing antibodies (bnAbs) is critical effective vaccine, our goal was to assess ability bnAb B cell lineage-designed envelope SOSIP (protein stabilized by disulfide bond gp120-gp41-named "SOS"-and isoleucine-to-proline point...
Eliciting potent and broadly neutralizing antibodies (bnAbs) is a major goal in HIV-1 vaccine development. Here, we describe how germline-targeting immunogen BG505 SOSIP germline trimer 1.1 (GT1.1), generated through structure-based design, engages diverse range of VRC01-class bnAb precursors. A single immunization with GT1.1 expands CD4 binding site (CD4bs)–specific B cells knock-in mice drives maturation. In nonhuman primates (NHPs), primes CD4bs-specific serum responses. Selected...
Journal Article Cytomegalovirus (CMV)-Specific Intravenous Immunoglobulin for the Prevention of Primary CMV Infection and Disease after Marrow Transplant Get access Raleigh A. Bowden, Bowden Fred Hutchinson Cancer Research Center Departments Pediatrics, Medicine, Biostatistics, University Washington, Seattle Search other works by this author on: Oxford Academic PubMed Google Scholar Lloyd D. Fisher, Fisher Karin Rogers, Rogers Monica Cays, Cays Joel Meyers The Infectious Diseases, Volume...
Abstract Ig Fc receptors bind to immune complexes through interactions with the regions of specific Ab subclasses initiate or inhibit defense mechanisms leukocytes on which they are expressed. The mechanism action IgG-based therapeutic molecules, routinely evaluated in nonhuman primate models, involves binding low-affinity FcRIII (CD16). premise that IgG/CD16 primates mimic those present humans has not been evaluated. Therefore, we have identified and characterized CD16 associated TCR...
PD-1 expression is generally associated with exhaustion of T cells during chronic viral infections based on the finding that expressing respond poorly to antigen activation and blockade PD-1/PD-ligand interaction restores such specific responses in vitro. We tested this hypothesis by examining virus-specific CD8 total vivo determine whether constitutes a reliable marker immune SIV infection. The Ki67 was monitored longitudinally cell subsets peripheral blood, bone marrow, lymph node rectal...
Chronic immune activation (IA) is considered as the driving force of CD4+ T cell depletion and AIDS. Fundamental clues in mechanisms that regulate IA could lie natural hosts SIV, such African green monkeys (AGMs). Here we investigated role innate cells IFN-α control AGMs. AGMs displayed significant NK upon SIVagm infection, which was correlated with levels IFN-α. Moreover, detected cytotoxic lymph nodes during early acute phase infection. Both plasmacytoid myeloid dendritic (pDC mDC) homing...
The programmed cell death-1 (PD-1)/programmed death ligand-1 pathway has been shown to limit cell-mediated effector functions during chronic viral infections impeding clearance of pathogens. As a strategy reverse this exhaustion and increase T polyfunctionality, PD-1 ligands were blocked in vivo using recombinant macaque fused Ig-Fc (rPD-1-Fc) SIVmac239-infected rhesus macaques the early phase infection, either alone or combination with antiretroviral therapy. In vitro blockade showed...
High-mannose-type glycans (HMGs) are aberrantly enriched on HIV envelope glycoproteins. However, there is currently no drug selectively targeting HIV-associated HMGs. Here, we describe a novel HMG-targeting "lectibody," recombinant Fc-fusion protein comprising human IgG1 Fc and actinohivin lectin variant (Avaren) obtained by structure-guided modifications for improved overall surface charge properties (AvFc). AvFc was engineered produced using rapid scalable plant-based transient...
Abstract Acute SIV infection is characterized by explosive of memory CD4 T cells in peripheral and mucosal tissues. Interestingly, relatively few are infected until as late days 7–8 after challenge. However, day 10 postinfection, most the carry viral DNA. The rapidity with which expands within 2–3 to encompass virtually entire cell compartment suggests significant alterations susceptibility during this period. mechanism(s) underlying increased permissiveness not known. In study, we show that...
Abstract Zika virus (ZIKV) is a mosquito-borne and sexually transmitted flavivirus that associated with fetal CNS-damaging malformations during pregnancy in humans. This study documents the viral kinetics immune responses rhesus macaques infected clinical ZIKV Brazilian isolate. We evaluated induced after an i.v. infection isolate (HS-2015-BA-01) for up to 142 d. ZIKV-specific Ab-secreting cells, germinal center reactions, monocyte, dendritic cell, NK, T cell frequencies were monitored....
Authorization of the Matrix-M (MM)–adjuvanted R21 vaccine by three countries and its subsequent endorsement World Health Organization for malaria prevention in children are a milestone fight against malaria. Yet, our understanding innate adaptive immune responses elicited this remains limited. Here, we compared clinically relevant adjuvants [3M-052 + aluminum hydroxide (Alum) (3M), TLR7/8 agonist formulated Alum; GLA-LSQ, TLR4 liposomes with QS-21; MM, now-approved adjuvant R21] their...
Summary Phenotypic and functional studies of the programmed death‐1 (PD‐1) molecule on CD4 + CD8 T cells were performed peripheral blood mononuclear from uninfected simian immunodeficiency virus (SIV)‐infected rhesus macaques. These data demonstrated a rapid upregulation PD‐1 expression tetramer‐positive MamuA.01 SIV‐infected macaques upon infection. Upregulation total was not detectable. In contrast, T‐cell markedly higher in during chronic, but acute, infection there correlation between...