A5027138031- HIV Research and Treatment
- HIV/AIDS Research and Interventions
- Immunotherapy and Immune Responses
- Immune Cell Function and Interaction
- Cytomegalovirus and herpesvirus research
- HIV/AIDS drug development and treatment
- Lymphatic System and Diseases
- Immunodeficiency and Autoimmune Disorders
- T-cell and B-cell Immunology
- Blood groups and transfusion
- Monoclonal and Polyclonal Antibodies Research
- Respiratory viral infections research
- Influenza Virus Research Studies
- HIV-related health complications and treatments
- Parvovirus B19 Infection Studies
- Herpesvirus Infections and Treatments
- Viral-associated cancers and disorders
- Neonatal Health and Biochemistry
- Viral Infections and Vectors
- Virology and Viral Diseases
- COVID-19 Impact on Reproduction
- Hepatitis B Virus Studies
- Lymphoma Diagnosis and Treatment
- Biosensors and Analytical Detection
- Complement system in diseases
Duke Medical Center
2020-2024
GlaxoSmithKline (United States)
2023-2024
Duke University
2020-2024
Smiths Detection (Canada)
2024
University of Virginia
2019
University of Pittsburgh
2013-2014
Institute for Medical Research
2008
Human cytomegalovirus (HCMV) is the most common congenital infection and a leading cause of stillbirth, neurodevelopmental impairment, pediatric hearing loss worldwide. Development maternal vaccine or therapeutic to prevent HCMV has been hindered by limited knowledge immune responses that protect against transmission in utero. To identify protective antibody responses, we measured HCMV-specific IgG binding antiviral functions paired cord blood sera from HCMV-seropositive transmitting (n =...
Adolescents are a growing population of people living with HIV. The period between weaning and sexual debut presents low-risk window for HIV acquisition, making early childhood an ideal time implementing immunization regimen. Because the elicitation broadly neutralizing antibodies (bnAbs) is critical effective vaccine, our goal was to assess ability bnAb B cell lineage-designed envelope SOSIP (protein stabilized by disulfide bond gp120-gp41-named "SOS"-and isoleucine-to-proline point...
Lymphatic and blood vessels are formed by specialized lymphatic endothelial cells (LEC) (BEC), respectively. These populations not only form peripheral tissue vessels, but also critical supporting structures in secondary lymphoid organs, particularly the lymph node (LN). Lymph LEC (LN-LEC) have been shown to important immunological functions that observed from lymphatics. LN-LEC can maintain tolerance through direct presentation of self-antigen via MHC-I, leading CD8 T cell deletion;...
ABSTRACT HIV-1-infected nonprogressors (NP) inhibit disease progression for years without antiretroviral therapy. Defining the mechanisms this resistance to could be important in determining strategies controlling HIV-1 infection. Here we show that two types of professional antigen-presenting cells (APC), i.e., dendritic (DC) and B lymphocytes, from NP lacked ability mediate trans infection CD4 + T cells. In contrast, APC progressors (PR) HIV-1-seronegative donors (SN) were highly effective...
Uncovering the sanctuaries of long-lived HIV-1 reservoir is crucial to develop cure strategies. Pediatric immunity distinct from that adults, which may alter where established in infancy. Thus, it important utilize pediatric models inform cure-directed approaches for HIV-1-infected children. We used an infant rhesus macaque model infection via breastfeeding identify key sites viral persistence under antiretroviral therapy (ART). The gastrointestinal tract was found be a site low-level...
ABSTRACT Nipah virus nucleocapsid (NiV-N) protein was expressed in Escherichia coli and purified by histidine tag-based affinity chromatography. An indirect immunoglobulin G (IgG) enzyme-linked immunosorbent assay (ELISA) for human swine sera an IgM capture ELISA were established using the recombinant NiV-N as antigen. One hundred thirty-three suspected patient 16 used to evaluate newly systems comparison with CDC inactivated-virus-based systems. For sera, protein-based IgG had a sensitivity...
Understanding viral rebound in pediatric HIV-1 infection may inform the development of alternatives to lifelong antiretroviral therapy (ART) achieve remission. We thus investigated after analytical treatment interruption (ATI) 10 infant macaques orally infected with SHIV.C.CH505 and treated long-term ART. Rebound viremia was detected within 7 35 days ATI 9 animals, posttreatment control seen 5 Mamu-A*01+ macaques. Single-genome sequencing revealed that initial virus similar DNA present CD4+...
To develop vaccines for certain key global pathogens such as HIV, it is crucial to elicit both neutralizing and non-neutralizing Fc-mediated effector antibody functions. Clinical evidence indicates that functions including antibody-dependent cellular cytotoxicity (ADCC) phagocytosis (ADCP) contribute protection against several pathogens. In this study, we demonstrated conjugation of HIV Envelope (Env) antigen gp120 a self-assembling nanofiber material named Q11 induced antibodies with higher...
While the benefits of early antiretroviral therapy (ART) initiation in perinatally infected infants are well documented, is not always possible postnatal pediatric HIV infections. The timing ART likely to affect size latent viral reservoir established, as development adaptive immune responses, such generation neutralizing antibody responses against virus. How these parameters impact ability control viremia and time rebound after interruption unclear has never been modeled infants. To...
Kaposi's sarcoma (KS) is an unusual neoplasia wherein the tumor consists primarily of endothelial cells infected with human herpesvirus 8 (HHV-8; sarcoma-associated herpesvirus) that are not fully transformed but instead driven to excess proliferation by inflammatory and angiogenic factors. This oncogenic process has been postulated unproven depend on a paracrine effect abnormal host cytokines chemokines produced HHV-8-infected B lymphocytes. Using newly developed measures for intracellular...
Abstract Viral dynamics of acute HIV infection and rebound following suspension antiretroviral therapy may be qualitatively similar but must differ given, for one, development adaptive immune responses. Understanding the differences viral in pediatric populations provide insights into mechanisms control with potential implications vaccine design effective targeted therapeutics infants children. Mathematical models have been a crucial tool to elucidate complex processes driving infections...
To eliminate vertical HIV transmission and achieve therapy-free viral suppression among children living with HIV, novel strategies beyond antiretroviral therapy (ART) are necessary. Our group previously identified a triple broadly neutralizing antibody (bNAb) combination comprising of 3BNC117, PGDM1400 PGT151 that mediates robust in vitro neutralization non-neutralizing effector functions against cross-clade panel simian human immunodeficiency viruses (SHIVs). In this study, we evaluated the...
CXCL13 is a constitutively expressed chemokine that controls migration of immune cells to lymphoid follicles. Previously, we found mRNA levels increased in rhesus macaque spleen tissues during AIDS. This led us examine the and locations by detailed situ methods cynomolgus intestinal tissues. Our results revealed there were distinct localization patterns compared protein germinal centers. These shifted course simian immunodeficiency virus (SIV) infection, with expression within around...
Daily burden and clinical toxicities associated with antiretroviral therapy (ART) emphasize the need for alternative strategies to induce long-term human immunodeficiency virus (HIV) remission upon ART cessation. Broadly neutralizing antibodies (bNAbs) can both neutralize free virions mediate effector functions against infected cells therefore represent a leading immunotherapeutic approach. To increase potency breadth, as well limit development of resistant strains, it is likely that bNAbs...
A bstract While the benefits of early antiretroviral therapy (ART) initiation in perinatally infected infants are well documented, ART is not always possible postnatal pediatric HIV infections, which account for majority cases worldwide. The timing onset likely to affect size latent viral reservoir established, as development adaptive immune responses, such generation neutralizing antibody responses against virus. How these parameters impact ability control viremia and time rebound after...
Women of childbearing age are three times more likely to contract HIV infection than their male counterparts. Poor testing rates coupled with low adherence antiretroviral therapy (ART) result in a high risk mother-to-infant transmission, especially during the breastfeeding period.
ABSTRACT To eliminate vertical HIV transmission and achieve therapy-free viral suppression among children living with HIV, novel strategies beyond antiretroviral therapy (ART) are necessary. Our group previously identified a triple bNAb combination comprising of 3BNC117, PGDM1400 PGT151 that mediates robust in vitro neutralization non-neutralizing effector functions against cross-clade panel simian human immunodeficiency viruses (SHIVs). In this study, we evaluated the safety,...
A bstract The presence of antibodies against HIV in infected children is associated with a greater capacity to control viremia the absence therapy. While benefits early antiretroviral treatment (ART) infants are well documented, ART may interfere development antibody responses. In contrast adults, treated lack detectable HIV-specific antibodies, suggesting fundamental difference pathogenesis. Despite this potential adverse effect, decrease size latent reservoir established infection infants,...
Abstract Background In 2022, UNAIDS estimated out of the 1.7 million children living with HIV, only 57.1% had access to antiretroviral therapy (ART). Though ART has significantly decreased HIV-associated morbidity and mortality, it cannot eliminate latent viral reservoir. Consequently, most people HIV demonstrate rebound when is interrupted. There therefore a critical need for alternative therapeutic achieve long term free control. Broadly neutralizing antibodies (bnAb) have shown promising...