A5058974512- Cytomegalovirus and herpesvirus research
- Herpesvirus Infections and Treatments
- Immunotherapy and Immune Responses
- HIV Research and Treatment
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Virus-based gene therapy research
- Parvovirus B19 Infection Studies
- Influenza Virus Research Studies
- Immune Response and Inflammation
- SARS-CoV-2 and COVID-19 Research
- Respiratory viral infections research
- Toxoplasma gondii Research Studies
- Monoclonal and Polyclonal Antibodies Research
- vaccines and immunoinformatics approaches
- Biosensors and Analytical Detection
- Animal Virus Infections Studies
- Cancer Immunotherapy and Biomarkers
- Viral gastroenteritis research and epidemiology
- Neonatal Health and Biochemistry
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Hepatitis B Virus Studies
- SARS-CoV-2 detection and testing
- Advanced biosensing and bioanalysis techniques
- Cancer Genomics and Diagnostics
IGM Biosciences (United States)
2022-2025
Inner Mongolia University
2025
The University of Texas Health Science Center at Houston
2019-2024
Fudan University Shanghai Cancer Center
2020-2024
Shanghai Medical College of Fudan University
2024
Shanghai Cancer Institute
2024
Brown Foundation
2019-2024
Henan Agricultural University
2024
Fudan University
2024
Shanghai Stomatological Hospital
2023
With accumulating evidence indicating the importance of cytotoxic T lymphocytes (CTLs) in containing human immunodeficiency virus–1 (HIV-1) replication infected individuals, strategies are being pursued to elicit virus-specific CTLs with prototype HIV-1 vaccines. Here, we report protective efficacy vaccine-elicited immune responses against a pathogenic SHIV-89.6P challenge rhesus monkeys. Immune were elicited by DNA vaccines expressing SIVmac239 Gag and 89.6P Env, augmented administration...
ABSTRACT Cellular immune responses, particularly those associated with CD3 + CD8 cytotoxic T lymphocytes (CTL), play a primary role in controlling viral infection, including persistent infection human immunodeficiency virus type 1 (HIV-1). Accordingly, recent HIV-1 vaccine research efforts have focused on establishing the optimal means of eliciting such antiviral CTL responses. We evaluated several DNA formulations, modified vaccinia Ankara vector, and replication-defective adenovirus...
The prophylactic efficacy of DNA and replication-incompetent adenovirus serotype 5 (Ad5) vaccine vectors expressing simian immunodeficiency virus (SIV) Gag was examined in rhesus macaques using an SIVmac239 challenge. Cohorts either Mamu-A*01(+) or Mamu-A*01(-) were immunized with a prime-Ad5 boost regimen; for comparison, third cohort consisting monkeys the Ad5 vector alone both prime boost. All animals, along unvaccinated control cohorts macaques, challenged intrarectally SIVmac239. Viral...
DNA vaccination is an effective means of eliciting both humoral and cellular immunity, including cytotoxic T lymphocytes (CTL). Using influenza virus model, we previously demonstrated that injection encoding nucleoprotein (NP) induced major histocompatibility complex class I-restricted CTL cross-strain protection from lethal challenge in mice (J. B. Ulmer et al., Science 259:1745-1749, 1993). In the present study, have characterized more detail immune responses by NP DNA, which included...
Antibodies are an important component in host immune responses to viral pathogens. Because of their unique maturation process, antibodies can evolve be highly specific antigens. Physicians and researchers have been relying on such high specificity quest understand host-viral interaction pathogenesis mechanisms find potential cures for infection disease. With more than 60 recombinant monoclonal developed human use the last 20 years, now considered a viable therapeutic modality infectious...
Significance Congenital human cytomegalovirus (HCMV) infection is an important cause of newborn disability, and developing a vaccine against congenital HCMV top priority. However, despite decades efforts, remains elusive. Previous vaccines lacked antigen called pentameric glycoprotein H (gH) complex, essential for the virus to infect epithelial/endothelial cells, these induced poor neutralizing antibodies. To support unique concept featuring gH we established 45 mAbs from rabbit immunized...
A new replication-defective human cytomegalovirus vaccine shows promise in protecting against this dangerous virus that can cause severe neurodevelopmental defects babies.
Recent approval of mRNA vaccines for emergency use against COVID-19 is likely to promote rapid development mRNA-based targeting a wide range infectious diseases. Compared conventional approaches, this vaccine modality promises comparable potency while substantially accelerating the pace and deployment doses. Already demonstrated successfully single antigen such as COVID-19, technology could be optimized complex multi-antigen vaccines. Herein, utilizing multiple influenza antigens, we...
Abstract Emergency use authorization of COVID vaccines has brought hope to mitigate pandemic coronavirus disease 2019 (COVID-19). However, there remains a need for additional effective meet the global demand and address potential new viral variants. mRNA technologies offer an expeditious path alternative traditional vaccine approaches. Here we describe efforts utilize platform rational design evaluations candidates based on spike (S) glycoprotein SARS-CoV-2. Several constructs S-protein,...
Vaccination of nonhuman primates with SARS-CoV-2 spike trimers and AS03 adjuvant induces neutralizing antibodies sufficient to protect from disease.
Digital pathology allows computerized analysis of tumor ecosystem using whole slide images (WSIs). Here, we present single-cell morphological and topological profiling (sc-MTOP) to characterize by extracting the features nuclear morphology intercellular spatial relationship for individual cells. We construct a atlas comprising 410 million cells from 637 breast cancer WSIs dissect phenotypic diversity within tumor, inflammatory stroma respectively. Spatially-resolved identifies recurrent...
ABSTRACT Recent studies of human immunodeficiency virus (HIV)-specific CD8 + T cells have focused on responses to single, usually HLA-A2-restricted epitopes as surrogate measures the overall response HIV. However, assumption that a one epitope is representative total unconfirmed. Here we assess immunodominance and HIV-specific T-cell complexity using cytokine flow cytometry examine in 11 HLA-A2 HIV individuals. Initial demonstrated only 4 patients recognized putative immunodominant p17...
ABSTRACT The potential contribution of a plasmid DNA construct to vaccine-elicited protective immunity was explored in the simian immunodeficiency virus (SIV)/macaque model AIDS. Making use soluble major histocompatibility class I/peptide tetramers and peptide-specific killing assays monitor CD8 + T-lymphocyte responses dominant SIV Gag epitope genetically selected rhesus monkeys, codon-optimized gag vaccine shown elicit high-frequency SIV-specific cytotoxic (CTL) response. This CTL response...
DNA immunization offers a novel means to induce cellular immunity in population with heterogeneous genetic background. An immunorecessive cytotoxic T-lymphocyte (CTL) epitope influenza virus nucleoprotein (NP), residues 218 226, was identified when mice were immunized plasmid encoding full-length mutant NP which the anchor for immunodominant NP147-155 altered. Mice wild-type or protected from lethal cross-strain challenge, and protection could be adoptively transferred by immune splenocytes,...
Programmed Cell Death 1 (PD-1) plays a crucial role in immunomodulation. Binding of PD-1 to its ligand receptors down-regulates immune responses, and published reports suggest that this modulation is exploited cases tumor progression or chronic viral infection evade surveillance. Thus, blockade signal could restore enhance host functions. To test hypothesis, we generated panel mAbs specific human block PD tested them for vitro binding, blocking, functional T cell evaluated lead candidate two...
Human cytomegalovirus (HCMV) is the leading cause of congenital viral infection, and developing a prophylactic vaccine high priority to public health. We recently reported replication-defective human with restored pentameric complex glycoprotein H (gH)/gL/pUL128-131 for prevention HCMV infection. While quantity vaccine-induced antibody responses can be measured in neutralization assay, assessing quality such responses, including ability antibodies cross-neutralize field strains HCMV, remains...