A5039042920- Intensive Care Unit Cognitive Disorders
- Respiratory Support and Mechanisms
- Anesthesia and Sedative Agents
- Adenosine and Purinergic Signaling
- Long-Term Effects of COVID-19
- COVID-19 Clinical Research Studies
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Renal Transplantation Outcomes and Treatments
- Family and Patient Care in Intensive Care Units
- SARS-CoV-2 and COVID-19 Research
- Neuroinflammation and Neurodegeneration Mechanisms
- Pancreatic function and diabetes
- Barrier Structure and Function Studies
- Neonatal Respiratory Health Research
- Hemodynamic Monitoring and Therapy
- Anesthesia and Neurotoxicity Research
- Hematopoietic Stem Cell Transplantation
- Diabetes and associated disorders
- Safe Handling of Antineoplastic Drugs
- Protein purification and stability
- Hereditary Neurological Disorders
- Healthcare Decision-Making and Restraints
- Extracellular vesicles in disease
- Eosinophilic Esophagitis
Massachusetts General Hospital
2017-2022
Harvard University
2007-2022
Pain and Rehabilitation Medicine
2022
National Heart Lung and Blood Institute
2020-2022
Brigham and Women's Hospital
2007-2020
National Institute of General Medical Sciences
2018
Beth Israel Deaconess Medical Center
2008-2012
Miami Transplant Institute
2012
The relationship between SARS-CoV-2 viral load and risk of disease progression remains largely undefined in coronavirus 2019 (COVID-19). Here, we quantify from participants with a diverse range COVID-19 severity, including those requiring hospitalization, outpatients mild disease, individuals resolved infection. We detected plasma RNA 27% hospitalized participants, 13% diagnosed COVID-19. Amongst the COVID-19, report that higher prevalence detectable is associated worse respiratory lower...
We have shown that CD39 and CD73 are coexpressed on the surface of murine CD4+ Foxp3+ regulatory T cells (Treg) generate extracellular adenosine, contributing to Treg immunosuppressive activity. now describe CD39, independently CD73, is expressed by a subset blood-derived human CD25+ CD127lo Treg, defined robust expression Foxp3. A further distinct population CD39+ lymphocytes can be identified, which do not express CD25 FoxP3 exhibit memory effector cellular phenotype. Differential...
To the Editor Management of patient sedation and analgesia to alleviate anxiety pain facilitate mechanical ventilation is one key roles every intensivist. During coronavirus disease 2019 (COVID-19) pandemic, unprecedented numbers patients require in intensive care units (ICUs) other hospital locations due their ventilator dependence. However, pharmacologic mechanically ventilated with COVID-19 has thus far received very little attention critical literature, minimal mention Society Critical...
Oxygen is the most commonly used therapy in hospitalized patients with COVID-19. In those who develop worsening pneumonia and acute respiratory distress syndrome (ARDS), high concentrations of oxygen may need to be administered for prolonged time periods, often together mechanical ventilation. Hyperoxia, although lifesaving essential maintaining adequate oxygenation short term, have adverse long-term consequences upon lung parenchymal structure function. How hyperoxia per se impacts disease...
Purinergic signaling and associated ectonucleotidases, such as CD39 CD73, have been implicated in the pathogenesis of inflammatory bowel disease (IBD). is known to be a Treg memory cell marker, here we determine phenotype function CD73(+) CD4(+) T lymphocytes patients with IBD. We describe elevated levels cells peripheral blood intestinal lamina propria active The functional these was further determined by gene expression, ecto-enzymatic activity, suppressive assays. Increased numbers...
Abstract The relationship between SARS-CoV-2 viral load and risk of disease progression remains largely undefined in coronavirus 2019 (COVID-19). We quantified from participants with a diverse range COVID-19 severity, including those requiring hospitalization, outpatients mild disease, individuals resolved infection. plasma RNA was detected 27% hospitalized 13% diagnosed COVID-19. Amongst the COVID-19, higher prevalence detectable associated worse respiratory lower absolute lymphocyte...
The promise of islet cell transplantation cannot be fully realized in the absence improvements engraftment resilient islets. marginal mass islets surviving serial peritransplant insults may lead to exhaustion and thereby contribute an unacceptably high rate intermediate long-term graft loss. Hence, we have studied effects treatment with alpha 1-antitrypsin (AAT) a syngeneic nonautoimmune model. A number mouse were transplanted into diabetic hosts function was analyzed control AAT treated...
Background Previously, we have demonstrated that short-term treatment of new onset diabetic Non-obese (NOD) mice, mice are afflicted with both type 1 (T1D) and 2 (T2D) diabetes either Power Mix (PM) regimen or alpha1 antitrypsin (AAT) permanently restores euglycemia, immune tolerance to self-islets normal insulin signaling. Methodology Principal Findings To search for relevant therapeutic targets, applied genome wide transcriptional profiling systems biology oriented bioinformatics analysis...
Preliminary reports suggest that critically ill patients with coronavirus disease 2019 (COVID-19) infection requiring mechanical ventilation may have markedly increased sedation needs compared ill, mechanically ventilated without COVID-19. We conducted a study to examine sedative use for this patient population within multiple intensive care units (ICUs) of large academic medical center. A retrospective, single-center cohort practices COVID-19 during the first 10 days was in 8 ICUs at...
Critically ill patients are routinely exposed to high concentrations of supplemental oxygen for prolonged periods time, which can be life-saving in the short term, but such exposure also causes severe lung injury and increases mortality. To address this therapeutic dilemma, we studied mechanisms tissue-damaging effects mice. We show that pulmonary invariant natural killer T (iNKT) cells unexpectedly crucial development acute oxygen-induced injury. iNKT express ectonucleotidase CD39,...
Abstract Oscillatory blood flow in the microcirculation is generally considered to be result of cardiopulmonary influences or active vasomotion. In this report, we describe rhythmically oscillating bridging vessels mouse colon that appeared independent known biological control mechanisms. Corrosion casting and scanning electron microscopy demonstrated highly branched connected submucosal with mucosal plexus. Because similar morphometric characteristics (19 ± 11 μm vs. 28 16 μm), arterioles...
Abstract Prolonged exposure to hyperoxia has deleterious effects on the lung, provoking both inflammation and alveolar injury. The elements of hyperoxic injury, which result in high rates lethality experimental models, are thought include multicellular immune responses. To characterize these alterations cell populations, we performed time-of-flight mass cytometry (CyTOF) analysis CD45-expressing cells whole lung parenchyma bronchoalveolar space mice, exposed 48 hours together with normoxic...
ABSTRACT The relationship between SARS-CoV-2 viral load and risk of disease progression remains largely undefined in coronavirus 2019 (COVID-19). We quantified from participants with a diverse range COVID-19 severity, including those requiring hospitalization, outpatients mild disease, individuals resolved infection. plasma RNA was detected 27% hospitalized 13% diagnosed COVID-19. Amongst the COVID-19, higher prevalence detectable associated worse respiratory lower absolute lymphocyte...
Abstract Rhythmic changes in blood flow direction have been described the mucosal plexus of mice with acute colitis. In this report, we studied colitis induced either by dextran sodium sulfate or trinitrobenzenesulfonic acid. Both forms were associated oscillations as documented fluorescence intravital videomicroscopy. The complex oscillation patterns suggested more than one mechanism for these flow. By tracking fluorescent nanoparticles inflamed plexus, identified two within inflammatory...
To describe sedative and analgesic drug utilization in a cohort of critically ill patients with coronavirus disease 2019 compare standard sedation an alternative approach using inhaled isoflurane.This was retrospective study designed to doses sedatives between ICU receiving IV mixed including isoflurane. Data were obtained from electronic medical records.ICU at large academic center where mechanical ventilation delivered Draeger Apollo (Draeger Medical, Telford, PA) anesthesia...
Quantitative assessment of microvascular structure is relevant to the investigations ischemic injury, reparative angiogenesis and tumor revascularization. In light microscopy applications, thick tissue specimens are necessary characterize networks; however, leads image distortions due out-of-focus light. Structured illumination confocal an optical sectioning technique that improves contrast resolution by using a grid pattern identify plane-of-focus within specimen. Because structured can be...
See Article, page 671 The twenty-first century saw the rise of "systems biology," an interdisciplinary approach in biomedical research integrating computational modeling, cell biology, proteomics, and genomics to provide understanding larger picture a tissue or organism.1 This Open Mind article focuses on rapidly evolving areas critical care anesthesiology, including use novel molecular markers for disease diagnosis phenotyping, predictive models perioperative risk assessment. Taken...
Certain forms of inflammation an allograft are highly detrimental to the induction and maintenance transplant tolerance as they foster stable commitment graft-destructive, not graft-protective, T-cell immunity. Hence, a reduction in adverse tissue may prove crucial facilitating long-lasting state tolerance.