A5103045589- SARS-CoV-2 and COVID-19 Research
- T-cell and B-cell Immunology
- COVID-19 Clinical Research Studies
- vaccines and immunoinformatics approaches
- Monoclonal and Polyclonal Antibodies Research
- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- Long-Term Effects of COVID-19
- Reproductive Biology and Fertility
- CAR-T cell therapy research
- Viral Infections and Outbreaks Research
- Immune responses and vaccinations
- Viral gastroenteritis research and epidemiology
- Ovarian cancer diagnosis and treatment
- Adolescent Sexual and Reproductive Health
- Ferroptosis and cancer prognosis
- Neuroinflammation and Neurodegeneration Mechanisms
- Insect symbiosis and bacterial influences
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- SARS-CoV-2 detection and testing
- Reproductive System and Pregnancy
- Sperm and Testicular Function
- Cancer-related molecular mechanisms research
- Reproductive tract infections research
- Clostridium difficile and Clostridium perfringens research
Harvard University
2015-2024
Brigham and Women's Hospital
2014-2024
Zhongshan People's Hospital
2024
Guangzhou University of Chinese Medicine
2023
Ragon Institute of MGH, MIT and Harvard
2022
Concord Consortium
2020
Zhejiang University
2013
The global coronavirus disease 2019 (COVID-19) pandemic caused by severe acute respiratory syndrome 2 (SARS-CoV-2) has made the development of a vaccine top biomedical priority. In this study, we developed series DNA candidates expressing different forms SARS-CoV-2 spike (S) protein and evaluated them in 35 rhesus macaques. Vaccinated animals humoral cellular immune responses, including neutralizing antibody titers at levels comparable to those found convalescent humans macaques infected...
Understanding humoral responses to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is critical for improving diagnostics, therapeutics, and vaccines. Deep serological profiling of 232 disease 2019 (COVID-19) patients 190 pre–COVID-19 era controls using VirScan revealed more than 800 epitopes in the SARS-CoV-2 proteome, including 10 likely recognized by neutralizing antibodies. Preexisting antibodies ORF1, whereas only COVID-19 patient primarily spike protein nucleoprotein. A...
Key features of immune memory are greater and faster antigen-specific antibody responses to repeat infection. In the setting immune-evading viral evolution, it is important understand how far recognition stretches across variants when cells recalled action by invasions. It also recall influences longevity secreted responses. We analyzed SARS-CoV-2 variant recognition; dynamics B cells; over time after infection, vaccination, boosting. find that a two-dose vaccination regimen given natural...
CD4 + T cells are central to long-term immunity against viruses through the functions of helper 1 (T H 1) and follicular FH ) cell subsets. To better understand role these subsets in coronavirus disease 2019 (COVID-19) immunity, we conducted a longitudinal study severe acute respiratory syndrome 2 (SARS-CoV-2)–specific antibody responses convalescent individuals who seroconverted during first wave pandemic Boston, MA, USA, across range COVID-19 severities. Analyses spike (S) nucleocapsid (N)...
Neutralizing antibodies that recognize the SARS-CoV-2 spike glycoprotein are principal host defense against viral invasion. Variants of bear mutations allow escape from neutralization by many human antibodies, especially those in widely distributed (“public”) classes. Identifying neutralize these variants concern and determining their prevalence important goals for understanding immune protection. To determine Delta Omicron BA.1 variant specificity B cell repertoires established an initial...
The ability of immunoglobulin (Ig) to recognize pathogens is critical for optimal immune fitness. Early events that shape preimmune Ig repertoires, expressed on IgM+ IgD+ B cells as cell receptors (BCRs), are poorly defined. Here, we studied germ-free mice and conventionalized littermates explore the hypothesis symbiotic microbes help repertoire. Ig-binding assays showed exposure conventional microbial symbionts enriched frequencies antibacterial in intestine spleen. This enrichment affected...
Upon antigenic stimulation, naïve CD4+ T cells can give rise to phenotypically distinct effector helper and long-lived memory cells. We computationally reconstructed the in vivo trajectory of cell differentiation during a type I inflammatory immune response identified two paths for precursor central arising directly from Unexpectedly, our studies revealed heterogeneity among cells, which are typically considered homogeneous save their diverse receptor usage. Specifically, previously...
Background Polycystic ovary syndrome (PCOS) is a common heterogeneous disorder linked with endocrine and metabolic disturbances. The underlying mechanism of PCOS, especially its effect on oocyte competence, remains unclear. study aimed to identify abnormal follicular changes using multi-omics approach in fluid from PCOS patients determine their effects competence. Methods A total 36 women 35 without who underwent vitro fertilization embryo transfer were included the study. Cumulus cells...
ABSTRACT Memory B cell reserves can generate protective antibodies against repeated SARS-CoV-2 infections, but with an unknown reach from original infection to antigenically drifted variants. We charted memory receptor-encoded monoclonal (mAbs) 19 COVID-19 convalescent subjects spike (S) and found 7 major mAb competition groups epitopes recurrently targeted across individuals. Inclusion of published newly determined structures mAb-S complexes identified corresponding epitopic regions. Group...
Objective To screen out the potential prediction genes for nasopharyngeal carcinoma(NPC)from gene microarray data of NPC samples and then verify by cell experiments.Methods The dataset was downloaded from Gene Expression Omnibus,and limma package employed to differentially expressed genes.Weighted correlation network analysis used weighted co-expression analysis,and Venn diagram drawn find common genes.The ontology annotation Kyoto encyclopedia genomes pathway enrichment were performed...