Brenna McKaig
A5001907248- COVID-19 Clinical Research Studies
- SARS-CoV-2 and COVID-19 Research
- Long-Term Effects of COVID-19
- Neurosurgical Procedures and Complications
- Intracerebral and Subarachnoid Hemorrhage Research
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Cerebrospinal fluid and hydrocephalus
- Diverticular Disease and Complications
- Advanced Neuroimaging Techniques and Applications
- vaccines and immunoinformatics approaches
- COVID-19 Impact on Reproduction
- Advanced MRI Techniques and Applications
- Innovations in Medical Education
- SARS-CoV-2 detection and testing
- Empathy and Medical Education
- Clinical Reasoning and Diagnostic Skills
- Immune responses and vaccinations
- Immune cells in cancer
- Immunotherapy and Immune Responses
- Radiomics and Machine Learning in Medical Imaging
- Appendicitis Diagnosis and Management
- Acute Ischemic Stroke Management
- Viral Infections and Outbreaks Research
- Sepsis Diagnosis and Treatment
- MRI in cancer diagnosis
Harvard University
2020-2024
Massachusetts General Hospital
2020-2024
Massachusetts College of Pharmacy and Health Sciences
2021
Understanding humoral responses to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is critical for improving diagnostics, therapeutics, and vaccines. Deep serological profiling of 232 disease 2019 (COVID-19) patients 190 pre–COVID-19 era controls using VirScan revealed more than 800 epitopes in the SARS-CoV-2 proteome, including 10 likely recognized by neutralizing antibodies. Preexisting antibodies ORF1, whereas only COVID-19 patient primarily spike protein nucleoprotein. A...
Mechanisms underlying severe coronavirus disease 2019 (COVID-19) remain poorly understood. We analyze several thousand plasma proteins longitudinally in 306 COVID-19 patients and 78 symptomatic controls, uncovering immune non-immune linked to COVID-19. Deconvolution of our proteome data using published scRNA-seq datasets reveals contributions from circulating tissue cells. Sixteen percent display reduced inflammation yet comparably poor outcomes. Comparison who died severely ill survivors...
The hallmark of severe COVID-19 is an uncontrolled inflammatory response, resulting from poorly understood immunological dysfunction. We hypothesized that perturbations in FoxP3+ T regulatory cells (Treg), key enforcers immune homeostasis, contribute to pathology. Cytometric and transcriptomic profiling revealed a distinct Treg phenotype patients, with increase proportions intracellular levels the lineage-defining transcription factor FoxP3, correlating poor outcomes. These Tregs showed...
T cell-mediated immunity plays an important role in controlling SARS-CoV-2 infection, but the repertoire of naturally processed and presented viral epitopes on class I human leukocyte antigen (HLA-I) remains uncharacterized. Here, we report first HLA-I immunopeptidome two cell lines at different times post infection using mass spectrometry. We found peptides derived not only from canonical open reading frames (ORFs) also internal out-of-frame ORFs spike nucleocapsid captured by current...
BACKGROUND. SARS-CoV-2 plasma viremia has been associated with severe disease and death in COVID-19 small-scale cohort studies. The mechanisms behind this association remain elusive.
Rationale: Alveolar and endothelial injury may be differentially associated with coronavirus disease (COVID-19) severity over time. Objectives: To describe alveolar dynamics associations COVID-19 severity, cardiorenovascular injury, outcomes. Methods: This single-center observational study enrolled patients requiring respiratory support at emergency department presentation. More than 40 markers of (including receptor for advanced glycation endproducts [RAGE]), angiopoietin-2), renin, kidney...
Preexisting cross-coronavirus immunity influences the early development of SARS-CoV-2 humoral after COVID-19.
Background Portable, low-field-strength (0.064-T) MRI has the potential to transform neuroimaging but is limited by low spatial resolution and signal-to-noise ratio. Purpose To implement a machine learning super-resolution algorithm that synthesizes higher images (1-mm isotropic) from lower T1-weighted T2-weighted portable brain scans, making them amenable automated quantitative morphometry. Materials Methods An external high-field-strength data set isotropic scans Open Access Series of...
Mechanisms of neutrophil involvement in severe coronavirus disease 2019 (COVID-19) remain incompletely understood. Here, we collect longitudinal blood samples from 306 hospitalized COVID-19+ patients and 86 controls perform bulk RNA sequencing enriched neutrophils, plasma proteomics, high-throughput antibody profiling to investigate relationships between states severity. We identify dynamic switches six distinct subtypes. At days 3 7 post-hospitalization, with display a granulocytic...
Summary COVID-19 has caused over 1 million deaths globally, yet the cellular mechanisms underlying severe disease remain poorly understood. By analyzing several thousand plasma proteins in 306 patients and 78 symptomatic controls serial timepoints using two complementary approaches, we uncover host immune non-immune not previously linked to this disease. Integration of proteomics with nine published scRNAseq datasets shows that SARS-CoV-2 infection upregulates monocyte/macrophage,...
Spontaneous intracerebral hemorrhage (ICH) in the cerebellum has a poor short-term prognosis, whereas data on long-term case fatality and recurrent vascular events are sparse. Herewith, we aimed to assess recurrence rate of after first cerebellar ICH.
Point-of-care ultrasound (US) has been suggested as the primary imaging in evaluating patients with suspected diverticulitis. Discrimination between simple and complicated diverticulitis may help to expedite emergent surgical consults determine risk of complications. This study aimed to: (1) accuracy an US protocol (TICS) for diagnosing emergency department (ED) setting (2) assess ability TICS distinguish diverticulitis.Patients clinically who underwent a diagnostic computed tomography (CT)...
Many patients with intracerebral hemorrhage (ICH) develop intraventricular (IVH), which is associated higher mortality and worse clinical outcome. External ventricular drains (EVDs) are often placed, but there little data on how much benefit from this intervention. We explored the use, timing, location of EVD in ICH any association outcome.During study period, 2870 presented primary ICH, 2486 were included analyses. Overall, 73 (± 13) years old; 54% male, 46% had IVH. An was placed 29% IVH...
Background and Purpose: The computed tomography angiography spot sign is associated with hematoma expansion, case fatality, poor functional outcome in spontaneous supratentorial intracerebral hemorrhage (ICH). However, no data are available on the cerebellar ICH. Methods: We investigated consecutive patients ICH at 3 academic hospitals between 2002 2017. determined patient characteristics, expansion (>33% or 6 mL), rate of discharge 90-day outcome. Poor was defined as a modified Rankin...
Abstract Background Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) plasma viremia has been associated with severe disease and death in coronavirus 2019 (COVID-19) small-scale cohort studies. The mechanisms behind this association remain elusive. Methods We evaluated the relationship between SARS-CoV-2 viremia, outcome, inflammatory proteomic profiles a of COVID-19 emergency department participants. viral load was measured using qRT-PCR based platform. Proteomic data were...
Multiple studies have identified an association between neutrophils and COVID-19 disease severity; however, the mechanistic basis of this remains incompletely understood. Here we collected 781 longitudinal blood samples from 306 hospitalized + patients, 78 âˆ' acute respiratory distress syndrome 8 healthy controls, performed bulk RNA-sequencing enriched neutrophils, plasma proteomics, cfDNA measurements high throughput antibody profiling assays to investigate relationship neutrophil states...
The recent introduction of portable, low-field MRI (LF-MRI) into the clinical setting has potential to transform neuroimaging. However, LF-MRI is limited by lower resolution and signal-to-noise ratio, leading incomplete characterization brain regions. To address this challenge, advances in machine learning facilitate synthesis higher images derived from one or multiple scans. Here, we report extension a super-resolution (SR) algorithm synthesize 1 mm isotropic MPRAGE-like scans T1-weighted...
Rationale and Objectives Medical humanities are becoming increasingly popular, required, recognized as positively impacting medical education practice. However, the extent of teaching in schools is largely unknown. We aimed to review school curricula Canada, UK, US. Our secondary objective was compare inclusion with rankings schools. Methods searched curriculum websites all accredited US check which topics were taught, whether they mandatory or optional. then noted both by Times Higher...
Multiple studies have identified an association between neutrophils and COVID-19 disease severity; however, the mechanistic basis of this remains incompletely understood. Here we collected 781 longitudinal blood samples from 306 hospitalized + patients, 78 - acute respiratory distress syndrome 8 healthy controls, performed bulk RNA-sequencing enriched neutrophils, plasma proteomics, cfDNA measurements high throughput antibody profiling assays to investigate relationship neutrophil states...
Abstract Background and Aims: Many patients with intracerebral hemorrhage (ICH) develop intraventricular (IVH), which is associated higher mortality worse clinical outcome. External ventricular drains (EVDs) are often placed, but there little data on how much benefit from this intervention. We explored the use, timing, location of EVD in ICH any association Results During study period 2,870 presented primary 2,486 were included analyses. Overall, 73 (± 13) years old; 54% male, 46% had IVH....