A5039976666- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- Immunotherapy and Immune Responses
- vaccines and immunoinformatics approaches
- Viral gastroenteritis research and epidemiology
- Infection Control and Ventilation
- COVID-19 and healthcare impacts
- Viral Infections and Outbreaks Research
- Dental Research and COVID-19
- SARS-CoV-2 detection and testing
- Monoclonal and Polyclonal Antibodies Research
- Inhalation and Respiratory Drug Delivery
- Cervical Cancer and HPV Research
- Viral Infections and Vectors
- Long-Term Effects of COVID-19
- Rabies epidemiology and control
- Surgical Sutures and Adhesives
- Calpain Protease Function and Regulation
- Clinical Nutrition and Gastroenterology
- HIV Research and Treatment
- interferon and immune responses
- Corporate Taxation and Avoidance
- Respiratory viral infections research
- T-cell and B-cell Immunology
- Taxation and Compliance Studies
Boston University
2020-2024
University of Liverpool
2022
Universidad del Noreste
2020
University School
2020
Lancashire Teaching Hospitals NHS Foundation Trust
2006-2009
University of Florida
2007
Valdosta State University
2007
Abstract The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic has devastated global public health systems and economies, with over 52 million people infected, millions of jobs businesses lost, more than 1 deaths recorded to date. Contact surfaces contaminated droplets generated by infected persons through exhaling, talking, coughing sneezing is a major driver SARS-CoV-2 transmission, the virus being able survive on for extended periods time. To interrupt these chains...
Many studies have examined the impact of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants on neutralizing antibody activity after they become dominant strains. Here, we evaluate consequences further viral evolution. We demonstrate mechanisms through which SARS-CoV-2 receptor binding domain (RBD) can tolerate large numbers simultaneous escape mutations and show that pseudotypes containing up to seven mutations, as opposed one three found in previously studied concern, are...
Despite the vast diversity of antibody repertoire, infected individuals often mount responses to precisely same epitopes within antigens. The immunological mechanisms underpinning this phenomenon remain unknown. By mapping 376 immunodominant “public epitopes” at high resolution and characterizing several their cognate antibodies, we concluded that germline-encoded sequences in antibodies drive recurrent recognition. Systematic analysis antibody-antigen structures uncovered 18 human 21...
Neutralizing antibodies that recognize the SARS-CoV-2 spike glycoprotein are principal host defense against viral invasion. Variants of bear mutations allow escape from neutralization by many human antibodies, especially those in widely distributed (“public”) classes. Identifying neutralize these variants concern and determining their prevalence important goals for understanding immune protection. To determine Delta Omicron BA.1 variant specificity B cell repertoires established an initial...
There are a variety of methods routinely used in the sterilization medical devices using hydrogen peroxide (H 2 O ) including vaporization, plasma generation and ionization. Many these systems for validated bioburden reduction bacterial spores. Here, we explored benefits vaporized H (VHP) treatment N95 respirators emergency decontamination reuse to alleviate PPE shortages healthcare workers COVID-19 emergency. The factors that considered effective fit, filter efficiency...
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has led to an unprecedented global health crisis, resulting in a critical need for effective vaccines that generate protective antibodies. Protein subunit represent promising approach but often lack the immunogenicity required strong immune stimulation. To overcome this challenge, it is first demonstrated advanced biomaterials can be leveraged boost effectiveness of SARS-CoV-2 protein vaccines. Additionally, reported oxygen...
Monoclonal antibodies (mAbs) are an important class of antiviral therapeutics. MAbs highly selective, well tolerated, and have long in vivo half-life as the capacity to induce immune-mediated virus clearance. Their activities can be further enhanced by integration their variable fragments (Fvs) into bispecific (bsAbs), affording simultaneous targeting multiple epitopes improve potency breadth and/or mitigate against viral escape a single mutation. Here, we explore bsAb strategy for...
Abstract The SARS-CoV-2 viral spike (S) protein mediates attachment and entry into host cells is a major target of vaccine drug design. Potent neutralizing antibodies derived from closely related antibody heavy chain genes (IGHV3-53 or 3-66) have been isolated multiple COVID-19 convalescent individuals. These usually contain minimal somatic mutations bind the S receptor-binding domain (RBD) to interfere with cellular receptor angiotensin-converting enzyme 2 (ACE2). We used antigen-specific...
Licensed COVID-19 vaccines ameliorate viral infection by inducing production of neutralizing antibodies that bind the SARS-CoV-2 Spike protein and inhibit cellular entry. However, clinical effectiveness these is transitory as variants escape antibody neutralization. Effective solely rely upon a T cell response to combat could be transformational because they can utilize highly conserved short pan-variant peptide epitopes, but mRNA-LNP vaccine has not been shown provide effective...
Verapamil in Angina-Phear BuD m 741 reduction of glyceryl trinitrate intake on verapamil was 16.1 tablets a week, suggesting that used rather arbitrarily and not only for angina.Neither trial corresponds to the ordinary conditions ambulant treatment. Summary ConclusionsA double-blind is reported 20 outpatients with angina.The dose 80 mg.three times daily fortnight, similar period placebo tablets.No significant relief angina by found.I am grateful Dr.
A total of 100 Pseudomonas aeruginosa (P. aeruginosa) isolates were collected over a 1.5- year period from cases canine otitis. Sensitivities to enrofloxacin, marbofloxacin, and orbifloxacin determined using minimum inhibitory concentration testing (MICT). Isolates also tested for sensitivities enrofloxacin marbofloxacin disk-diffusion susceptibility (DDST). significantly more sensitive than (z = −4.57; P<0.05) or −5.02; P<0.05). Agreement was 87% between MICT DDST with...
ABSTRACT Memory B cell reserves can generate protective antibodies against repeated SARS-CoV-2 infections, but with an unknown reach from original infection to antigenically drifted variants. We charted memory receptor-encoded monoclonal (mAbs) 19 COVID-19 convalescent subjects spike (S) and found 7 major mAb competition groups epitopes recurrently targeted across individuals. Inclusion of published newly determined structures mAb-S complexes identified corresponding epitopic regions. Group...
Monoclonal antibodies are an efficacious therapy against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). However, rapid viral mutagenesis led to escape from most of these therapies, outlining the need for antibody cocktail with a broad neutralizing potency. Using unbiased interrogation memory B cell repertoire patients convalescent COVID-19, we identified human antiviral activity in vitro and efficacy vivo all tested SARS-CoV-2 variants concern, including Delta Omicron BA.1...
The emergence of highly transmissible severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants concern (VOCs) that are resistant to the current COVID-19 vaccines highlights need for continued development broadly protective future. Here, we developed two messenger RNA (mRNA)-lipid nanoparticle (LNP) vaccines, TU88mCSA and ALCmCSA, using ancestral SARS-CoV-2 spike sequence, optimized 5′ 3′ untranslated regions (UTRs), LNP combinations. Our data showed these nanocomplexes...
Abstract Effective intervention strategies are urgently needed to control the COVID-19 pandemic. Human angiotensin-converting enzyme 2 (ACE2) is a carboxypeptidase that forms dimer and serves as cellular receptor for SARS-CoV-2. It also key negative regulator of renin-angiotensin system (RAS), conserved in mammals, which modulates vascular functions. We report here properties trimeric ACE2 variant, created by structure-based approach, with binding affinity ~60 pM spike (S) protein...
Abstract Coronavirus disease 2019 continues to batter the world with unceasing introduction of new variants causative virus, SARS-CoV-2. In order understand differences in caused by concern and develop variant-specific vaccines, suitable small animal models are required that mimic progression humans at various stages life. this study, we compared dynamics infection two SARS-CoV-2 (Delta Omicron) aged (>1 year 3 months old) young (<5 weeks Syrian hamsters ( Mesocricetus auratus ). We...
Coronavirus disease 2019 (COVID-19) has had devastating effects worldwide, with particularly high morbidity and mortality in outbreaks on residential care facilities. Amantadine, originally licensed as an antiviral agent for therapy prophylaxis against influenza A virus, beneficial patients Parkinson’s is used treatment of disease, multiple sclerosis, acquired brain injury, various other neurological disorders. Recent observational data suggest inverse relationship between the use amantadine...
Abstract The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic has devastated global public health systems and economies, with over 23 million people infected, millions of jobs businesses lost, more than 800 000 deaths recorded to date. Contact surfaces contaminated droplets generated by infected persons through exhaling, talking, coughing sneezing is a major driver SARS-CoV-2 transmission, the virus being able survive on for extended periods time. To interrupt these...
Convalescent plasma has been used to treat many viral diseases including Ebola. The manufacture of a purified anti-Ebola virus (EBOV) intravenous immunoglobulin (IVIG) from pooled convalescent is described in this paper.An enzyme-linked immunosorbent assay (ELISA) targeting an EBOV surface glycoprotein antigen was determine the titer and anti-EBOV IVIG. Anti-EBOV IVIG also tested neutralization assays using vesicular stomatitis pseudovirion expressing on its with live EBOV. Finally, efficacy...
Following acute HCV infection, the virus establishes a chronic disease in majority of patients whilst few individuals clear infection spontaneously. The precise mechanisms that determine or spontaneous clearance are not completely understood but proposed to be driven by host and viral genetic factors as well encoded immunomodulatory proteins. Using HIV-1 LTR tool measure NF-κB activity, we identified E1E2 glycoproteins more so E2 protein down-modulates activation 293T, TZM-bl physiologically...
Abstract SARS-CoV-2 can be disinfected using ultraviolet-C (UVC) light. For effective inactivation strategies, design and implementation, knowledge of UVC wavelength sensitivity, disinfection rate the relevant pathogen are required. This study aimed to determine profile irradiation with different wavelengths. Specifically, determined dosage, levels, sensitivity SARS-CoV-2. Assessment (isolate USA/WA1-2020) at peak wavelengths 259, 268, 270, 275 280 nm was performed a plaque assay method. A...
Although next-generation sequencing (NGS) has been instrumental in determining the genomic sequences of emerging RNA viruses, de novo sequence determination often lacks sufficient coverage 5′ and 3′ ends viral genomes. Since genome viruses contain transcription replication promoters that are essential for propagation, a lack terminal information hinders efforts to study mechanisms re-emerging viruses. To circumvent this, we have developed novel method termed ViBE-Seq (Viral Bona Fide End...