A5042993609- Poxvirus research and outbreaks
- Virus-based gene therapy research
- Herpesvirus Infections and Treatments
- Bacteriophages and microbial interactions
- Cell Image Analysis Techniques
- Cellular transport and secretion
- Advanced Fluorescence Microscopy Techniques
- Advanced Electron Microscopy Techniques and Applications
- CRISPR and Genetic Engineering
- Plant Virus Research Studies
- Rabies epidemiology and control
- Erythrocyte Function and Pathophysiology
- interferon and immune responses
- Lipid Membrane Structure and Behavior
- Toxoplasma gondii Research Studies
- Phagocytosis and Immune Regulation
- Bacillus and Francisella bacterial research
- Immunotherapy and Immune Responses
- RNA Interference and Gene Delivery
- Viral Infectious Diseases and Gene Expression in Insects
- Virology and Viral Diseases
- Image Processing Techniques and Applications
- Mosquito-borne diseases and control
- Autophagy in Disease and Therapy
- SARS-CoV-2 and COVID-19 Research
University of Birmingham
2003-2025
MRC Laboratory for Molecular Cell Biology
2015-2024
University College London
2015-2024
Medical Research Council
2016-2021
General Motors (Canada)
2020
ETH Zurich
2008-2017
Medical College of Wisconsin
2003-2012
Board of the Swiss Federal Institutes of Technology
2010
University of London
1982
Imperial College London
1982
Viruses employ many different strategies to enter host cells. Vaccinia virus, a prototype poxvirus, enters cells in pH-dependent fashion. Live cell imaging showed that fluorescent virus particles associated with and moved along filopodia the body, where they were internalized after inducing extrusion of large transient membrane blebs. p21-activated kinase 1 (PAK1) was activated by endocytic process had general characteristics macropinocytosis. The induction blebs, event, infection all...
To enter host cells, vaccinia virus, a prototype poxvirus, can induce transient macropinocytosis followed by endocytic internalization and penetration through the limiting membrane of pinosomes fusion. Although mature virions (MVs) Western reserve (WR) strain do this in HeLa cells activating plasma blebbing, MVs from International Health Department-J were found to rapid formation (and lengthening) filopodia. When signaling pathways underlying these responses compared, differences observed at...
Isogenic cells in culture show strong variability, which arises from dynamic adaptations to the microenvironment of individual cells. Here we study influence cell population context, determines a single cell's microenvironment, image-based RNAi screens. We developed comprehensive computational approach that employs Bayesian and multivariate methods at single-cell level. applied these 45 RNA interference screens various sizes, including 7 druggable genome 2 genome-wide screens, analysing 17...
ABSTRACT Endocytosis plays a crucial role in receptor signalling. VEGFR2 (also known as KDR) and its ligand VEGFA are fundamental neovascularisation. However, our understanding of the endocytosis signalling remains limited. Despite existence diverse internalisation routes, only endocytic pathway for is clathrin-mediated pathway. Here, we show that this predominant route absence ligand. Intriguingly, induces new itinerary VEGFR2, macropinocytosis, which becomes prevalent presence ligand,...
For image-based infection biology, accurate unbiased quantification of host–pathogen interactions is essential, yet often performed manually or using limited enumeration employing simple image analysis algorithms based on segmentation. Host protein recruitment to pathogens refractory automated assessment due its heterogeneous nature. An intuitive intelligent program assess host within general cellular pathogen defense lacking. We present HRMAn (Host Response Microbe Analysis), an open-source...
Abstract Mpox is a zoonotic disease endemic to Central and West Africa. Since 2022, two human-adapted monkeypox virus (MPXV) strains have caused large outbreaks outside these regions. Tecovirimat the most widely used drug treat mpox. It blocks viral egress by targeting phospholipase F13; however, structural details are unknown, mutations in F13 gene can result resistance against tecovirimat, raising public health concerns. Here we report structure of an homodimer using X-ray crystallography,...
Synopsis It is widely accepted that axillary malodour attributable to the microbial biotransformation of odourless, natural secretions into volatile odorous products. Consequently, there a need understand ecology axilla in order deodorant products, which control action this region, can be developed appropriate manner. A detailed characterization microflora group human volunteers has been performed. The composed four principal groups bacteria (staphylococci, aerobic coryneforms, micrococci...
We report the results of experimental work on (a) influence temperature age-dependent survival and infectivity miracidia Schistosoma mansoni (b) relationship between snail age, size susceptibility to infection. The death rate declined exponentially with age where life-expectancy was maximal (approximately 16 h) at 15 degrees C. Infectivity also rapidly larval but, in contrast survival, infection a maximum 25 Snail shown be more closely correlated host rather than age. Susceptibility...