A5029069228- Cell Image Analysis Techniques
- Single-cell and spatial transcriptomics
- Cellular transport and secretion
- RNA Research and Splicing
- Gene Regulatory Network Analysis
- Caveolin-1 and cellular processes
- Lipid Membrane Structure and Behavior
- Epigenetics and DNA Methylation
- Advanced Fluorescence Microscopy Techniques
- DNA Repair Mechanisms
- RNA Interference and Gene Delivery
- CRISPR and Genetic Engineering
- Erythrocyte Function and Pathophysiology
- Genetics, Bioinformatics, and Biomedical Research
- Bioinformatics and Genomic Networks
- RNA and protein synthesis mechanisms
- Nuclear Structure and Function
- Virus-based gene therapy research
- Bacteriophages and microbial interactions
- Machine Learning in Bioinformatics
- Image Processing Techniques and Applications
- Ubiquitin and proteasome pathways
- Endoplasmic Reticulum Stress and Disease
- Genomics and Chromatin Dynamics
- interferon and immune responses
University of Zurich
2016-2025
Life Science Zurich
2015-2018
Maastricht University
2013-2016
Synapse (Netherlands)
2012-2016
ETH Zurich
1999-2013
University Medical Center Utrecht
2013
Maastricht University Medical Centre
2012
École Polytechnique Fédérale de Lausanne
2001-2011
Max Planck Institute of Molecular Cell Biology and Genetics
2003-2005
Max Planck Society
2004-2005
Simian virus 40 (SV40) utilizes endocytosis through caveolae for infectious entry into host cells. We found that after binding to caveolae, particles induced transient breakdown of actin stress fibers. Actin was then recruited virus-loaded as patches served sites “tail” formation. Dynamin II also transiently recruited. These events depended on the presence cholesterol and activation tyrosine kinases phosphorylated proteins in caveolae. They were necessary formation caveolae-derived endocytic...
Making multiplexed subcellular protein maps Being able to visualize localizations within cells and tissues by means of immuno-fluorescence microscopy has been key developments in cell biology beyond. Gut et al. present a high-throughput method that achieves the detection more than 40 different proteins biological samples across multiple spatial scales. This allows simultaneous quantification their expression levels thousands single cells; captures detailed distribution various compartments,...
Simian Virus 40 (SV40) has been shown to enter host cells by caveolar endocytosis followed transport via caveosomes the endoplasmic reticulum (ER). Using a caveolin-1 (cav-1)–deficient cell line (human hepatoma 7) and embryonic fibroblasts from cav-1 knockout mouse, we found that in absence of caveolae, but also wild-type fibroblasts, virus exploits an alternative, cav-1–independent pathway. Internalization was rapid (t1/2 = 20 min) cholesterol tyrosine kinase dependent independent clathrin,...
Enveloped viruses need to fuse with a host cell membrane in order deliver their genome into the cell. While some plasma membrane, many are endocytosed prior fusion. Specific cues endosomal microenvironment induce conformational changes viral fusion proteins leading and In present study we investigated entry of coronaviruses (CoVs). Using siRNA gene silencing, found that known be important for late maturation endosome-lysosome profoundly promote infection cells mouse hepatitis coronavirus...
Individual cells make decisions that are adapted to their internal state and surroundings, but how can reliably do this remains unclear. To study the information processing capacity of human cells, we conducted multiplexed quantification signaling responses markers cellular state. Signaling nodes in a network displayed adaptive processing, which led heterogeneous growth factor enabled capture partially nonredundant about Collectively, as multimodal percept gives individual large accurately...
Abstract Many membraneless organelles are liquid-like domains that form inside the active, viscoelastic environment of living cells through phase separation. To investigate potential coupling separation with cytoskeleton, we quantify structural correlations (stress granules) and cytoskeletal filaments (microtubules) in a human-derived epithelial cell line. We find microtubule networks substantially denser vicinity stress granules. When microtubules depolymerized, sub-units localize near...
Abstract Organoids generated from human pluripotent stem cells provide experimental systems to study development and disease, but quantitative measurements across different spatial scales molecular modalities are lacking. In this study, we multiplexed protein maps over a retinal organoid time course primary adult tissue. We developed toolkit visualize progenitor neuron location, the arrangements of extracellular subcellular components global patterning in each addition, single-cell...
A growing community is constructing a next-generation file format (NGFF) for bioimaging to overcome problems of scalability and heterogeneity. Organized by the Open Microscopy Environment (OME), individuals institutes across diverse modalities facing these have designed specification process (OME-NGFF) address needs. This paper brings together wide range those members describe cloud-optimized itself-OME-Zarr-along with tools data resources available today increase FAIR access remove barriers...
Abstract Understanding and predicting molecular responses in single cells upon chemical, genetic or mechanical perturbations is a core question biology. Obtaining single-cell measurements typically requires the to be destroyed. This makes learning heterogeneous perturbation challenging as we only observe unpaired distributions of perturbed non-perturbed cells. Here leverage theory optimal transport recent advent input convex neural architectures present CellOT, framework for response...
Using total internal reflection fluorescence microscopy (TIR-FM), recovery after photobleaching (FRAP), and other light techniques, we analyzed the dynamics, activation, assembly of caveolae labeled with fluorescently tagged caveolin-1 (Cav1). We found that when activated by simian virus 40 (SV40), a nonenveloped DNA uses for cell entry, fraction mobile was dramatically enhanced both in plasma membrane (PM) caveosome, an intracellular organelle functions as intermediate station caveolar...