A5051147730- Retinal Development and Disorders
- Pregnancy and preeclampsia studies
- Single-cell and spatial transcriptomics
- Cell Image Analysis Techniques
- Reproductive System and Pregnancy
- Advanced Fluorescence Microscopy Techniques
- Cancer-related molecular mechanisms research
- Photoreceptor and optogenetics research
- Circular RNAs in diseases
- CRISPR and Genetic Engineering
- Adipose Tissue and Metabolism
- Neuroscience and Neural Engineering
- RNA regulation and disease
- Birth, Development, and Health
- Genomics and Phylogenetic Studies
- Photochromic and Fluorescence Chemistry
- Microbial Natural Products and Biosynthesis
- Click Chemistry and Applications
- Laser-Ablation Synthesis of Nanoparticles
- Calcium signaling and nucleotide metabolism
- Advanced biosensing and bioanalysis techniques
- COVID-19 Impact on Reproduction
- Exercise and Physiological Responses
- Industrial Technology and Control Systems
- Molecular Communication and Nanonetworks
University of Basel
2022-2025
Institute of Molecular and Clinical Ophthalmology Basel
2019-2025
Inner Mongolia University for Nationalities
2018-2022
Creative Commons
2021
Weatherford College
2021
Shanghai Jiao Tong University
2020-2021
Fujian Medical University
2021
Ruijin Hospital
2021
Bridge University
2020
Tongji University
2020
Human organoids recapitulating the cell-type diversity and function of their target organ are valuable for basic translational research. We developed light-sensitive human retinal with multiple nuclear synaptic layers functional synapses. sequenced RNA 285,441 single cells from these at seven developmental time points periphery, fovea, pigment epithelium choroid light-responsive adult retinas, performed histochemistry. Cell types in matured vitro to a stable "developed" state rate similar...
Tetragonal barium titanate nanoparticles (BTNPs) have been exploited as nanotransducers owing to their piezoelectric properties, in order provide indirect electrical stimulation SH-SY5Y neuron-like cells. Following application of ultrasounds cells treated with BTNPs, fluorescence imaging ion dynamics revealed that the synergic is able elicit a significant cellular response terms calcium and sodium fluxes; moreover, tests appropriate blockers demonstrated voltage-gated membrane channels are...
Abstract Organoids generated from human pluripotent stem cells provide experimental systems to study development and disease, but quantitative measurements across different spatial scales molecular modalities are lacking. In this study, we multiplexed protein maps over a retinal organoid time course primary adult tissue. We developed toolkit visualize progenitor neuron location, the arrangements of extracellular subcellular components global patterning in each addition, single-cell...
Abstract The identification of brown adipose deposits in adults has led to significant interest targeting this metabolically active tissue for treatment obesity and diabetes. Improved methods the direct measurement heat production as signature function adipocytes (BAs), particularly at single cell level, would be substantial benefit these ongoing efforts. Here, we report first application a small molecule-type thermosensitive fluorescent dye, ERthermAC, monitor thermogenesis BAs derived from...
Mild heat stimulation of muscle cells within the physiological range represents an intriguing approach for modulation their functions. In this work, photothermal conversion was exploited to remotely stimulate striated by using gold nanoshells (NSs) in combination with near-infrared (NIR) radiation. Temperature increments approximately 5 °C were recorded intracellular fluorescent molecular thermometer and demonstrated efficiently induce myotube contraction. The mechanism at base phenomenon...
Interleukin (IL)-17A may be an underlying factor in the pathophysiology of chronic obstructive pulmonary disease (COPD). Anti-IL-17 monoclonal antibodies have been used successfully treating several immune-mediated inflammatory diseases. This phase 2, randomized, placebo-controlled, double-blind, parallel-group, proof-of-concept study is first clinical evaluating efficacy and safety anti-IL-17A antibody CNTO 6785 patients with symptomatic moderate-to-severe COPD. Patients were treated (n =...
Colorectal cancer (CRC) is the most common human digestive malignancy with a poor prognosis; pathophysiology of colon involves multiple linkages regulatory networks. Recently, thrombospondin 2 (THBS2) has been extensively studied for its role in progression. In this study, we evaluated expression THBS2 CRC tissues and possible mechanism by which regulates Our results showed that upregulation cell lines high was correlated overall survival. The vitro experimental data overexpression promoted...
Histology is the gold standard for analyzing tissue structure and cell morphology. Immunostaining on thin sections enables precise visualization of antigens proteins. However, cryosectioning small tissues such as organoids, spheroids, tumoroids there a lack standardized, time- cost-effective methods, limiting throughput analysis. Here, we have adapted to our previously developed HistoBrick approach, in which arrangement spatially controlled within arrayed mini-wells. By testing various...
Stargardt disease is a currently untreatable, inherited neurodegenerative that leads to macular degeneration and blindness due loss-of-function mutations in the ABCA4 gene. We have designed dual adeno-associated viral vector encoding split-intein adenine base editor correct most common mutation (c.5882G>A, p.Gly1961Glu). optimized editing human models, including retinal organoids, induced pluripotent stem cell-derived pigment epithelial (RPE) cells, as well adult explants RPE/choroid vitro....
ABSTRACT Stargardt disease is a currently untreatable, inherited neurodegenerative that leads to macular degeneration and blindness due loss-of-function mutations in the ABCA4 gene. We have designed dual adeno-associated viral vector split-intein adenine base-editing strategy correct most common mutation (c.5882G>A, p.G1961E). optimized base editing human models, including retinal organoids, iPSC-derived pigment epithelial (RPE) cells, as well adult retinal- RPE/choroid explants vitro....
Brown adipocytes (BAs) are endowed with a high metabolic capacity for energy expenditure due to their mitochondria content. While mitochondrial pH is dynamically regulated in response stimulation and, return, affects various processes, how during adrenergic stimulation-induced thermogenesis unknown. We aimed reveal the spatial and temporal dynamics of stimulated BAs mechanisms behind dynamic changes. A targeted pH-sensitive protein, mito-pHluorin, was constructed transfected BAs. Transfected...
A novel, Gram-stain-negative, aerobic, rod-shaped, motile and moderately halophilic bacterium, designated strain LCB169T, was isolated from a saline soil sample Gansu Province, PR China. The cells of LCB169T grew at 10–52 °C (optimum 30 °C), pH 6.0–10.0 8.0) in the presence 0–17 % (w/v) NaCl 10–15 %). Phylogenetic analysis based on 16S rRNA gene sequences concatenated rRNA, gyrB rpoD genes revealed that represented member genus Halomonas class Gammaproteobacteria . most closely related...
Abstract Recurrent pregnancy loss (RPL) is three or more times of consecutive spontaneous pregnancy. The underlying cause complicated and the etiology over 50% RPL patients unclear. In present study, bone marrow mesenchymal stem cells were isolated from CBA/J female mice exosomes cell culture medium by ultracentrifugation. paired with male DBA/2 to generate abortion prone mouse model, females BALB/c used as control. Exosomes injected through uterine horns into pregnant on day 4.5 gestation...
Organoids generated from human pluripotent stem cells (PSCs) provide experimental systems to study development and disease. However, we lack quantitative spatiotemporal descriptions of organoid that incorporate measurements across different molecular modalities. Here focus on the retina use a single-cell multimodal approach reconstruct retinal development. We establish an computational pipeline generate multiplexed spatial protein maps over time course primary adult retina, registering...
SUMMARY Human organoids that mirror their corresponding organs in cell-type diversity present an opportunity to perform large-scale screens for compounds protect disease-affected or damage healthy cell types. However, such have not yet been performed. Here, we generated 20,000 human retinal with GFP-labeled cone photoreceptors. Since degeneration of cones is a leading cause blindness, induced death and screened 2,707 known targets, those saved further damaged cones. We identified kinase...
Summary How closely human organoids recapitulate cell-type diversity and maturation of their target organs is not well understood. We developed retinal with multiple nuclear synaptic layers. sequenced the RNA 158,844 single cells from these at six developmental time points periphery, fovea, pigment epithelium choroid light-responsive adult retinas, performed histochemistry. Cell types in matured vitro to a stable ‘developed’ state rate similar retina development vivo transcriptomes organoid...
Abstract Long non-coding RNAs (lncRNAs) have been ascertained as vital modulators in abdominal aortic aneurysm (AAA) development. In this research, the function and molecular mechanisms of lncRNA X-inactive specific transcript (XIST) evolution vascular smooth muscle cells (VSMCs) were assessed. Results showed that XIST expression was increased but miR-1264 level reduced serum AAA patients. depletion impeded human aorta VSMCs (HA-VSMCs’) ability to proliferate stimulate apoptosis, while...
How closely human organoids recapitulate cell-type diversity and maturation of their target organs is not well understood. We developed retinal with multiple nuclear synaptic layers. sequenced the RNA 158,844 single cells from these at six developmental time points periphery, fovea, pigment epithelium choroid light-responsive adult retinas, performed histochemistry. Cell types in matured vitro to a stable 'developed' state rate similar retina development vivo transcriptomes organoid cell...
A novel endophytic actinomycete, designated A301T, was isolated from the root of Geranium carolinianum Linn collected Mount Emei in China and characterized using a polyphasic approach. Growth occurred at 10-37 °C, pH 6-11 presence 0-5 % NaCl (w/v). Strain A301T contained ll-diaminopimelic acid as diagnostic diamino cell-wall peptidoglycan. The whole-cell hydrolysates included galactose ribose. predominant menaquinones were MK-9(H6) MK-9(H8). major cellular fatty acids C15 : 0, C16...
Abstract As the most common malignancy, lung cancer is characterised by high rates of occurrence and mortality. Although circular RNAs (circRNAs) are known to act as important regulators in cancer, their role remains poorly understood. In this study, circ_GRHPR expression was found be significantly upregulated serum five patients with non‐small cell (NSCLC), compared that healthy controls. It expressed at levels NSCLC lines, revealed qRT‐PCR analysis. Functionally, we demonstrated promotes...
The effect of intravascular interventional embolization and surgical operation on matrix metalloprotein (MMP)-2, MMP-9 caspase3 in serum intracranial aneurysms was analyzed to study the mechanisms. Seventy-nine patients from September 2011 August 2014 were divided into intervention group (n=41) craniotomy (n=38) based treatment methods, 40 cases normal volunteers with physical examination selected as control group. Patients treated embolization, no performed survival rate adverse reaction...
Organic dyes are useful tools for sensing cellular activities but unfavorable in single-molecule imaging, whereas quantum dots (QDs) widely applied imaging with few applications. Here, to visualize by monitoring the response of a single probe living cells, we propose bottom-up approach generate nanoprobes where four organic conjugated tetravalent single-chain avidin (scAVD) proteins via an intracellular click reaction. We demonstrate that nanoprobes, exhibiting increased brightness and...
Ca2+ distribution is spatially and temporally non-uniform inside cells due to cellular compartmentalization. However, sensing with small organic dyes, such as fura-2 fluo-4, has been practically applied at a single cell level where the averaged signal from freely diffusing dye molecules acquired. In this study, we aimed target azide-functionalized (N3-fura-2) specific site of subcellular compartments realize focal sensing. Using scAVD (single-chain avidin)–biotin interaction copper-free...