A5065563711- Neuroscience and Neuropharmacology Research
- Retinal Development and Disorders
- Photoreceptor and optogenetics research
- Neural dynamics and brain function
- Neuroscience and Neural Engineering
- Axon Guidance and Neuronal Signaling
- Plant Reproductive Biology
- Cell Image Analysis Techniques
- Single-cell and spatial transcriptomics
- Advanced Fluorescence Microscopy Techniques
- Neurogenesis and neuroplasticity mechanisms
- Nerve injury and regeneration
- Photoacoustic and Ultrasonic Imaging
- EEG and Brain-Computer Interfaces
- Multisensory perception and integration
- Bat Biology and Ecology Studies
- Animal Vocal Communication and Behavior
- Cell Adhesion Molecules Research
- Biochemical effects in animals
- Virus-based gene therapy research
- Traumatic Brain Injury Research
- Ophthalmology and Eye Disorders
- Cerebral Palsy and Movement Disorders
- Phytoplasmas and Hemiptera pathogens
- Plant Taxonomy and Phylogenetics
McGill University
2012-2023
Friedrich Miescher Institute
2017-2023
Institute of Molecular and Clinical Ophthalmology Basel
2019-2023
University of Basel
2020-2023
Montreal Neurological Institute and Hospital
2012-2023
Kiel University
2018
Humboldt-Universität zu Berlin
2010-2011
Human organoids recapitulating the cell-type diversity and function of their target organ are valuable for basic translational research. We developed light-sensitive human retinal with multiple nuclear synaptic layers functional synapses. sequenced RNA 285,441 single cells from these at seven developmental time points periphery, fovea, pigment epithelium choroid light-responsive adult retinas, performed histochemistry. Cell types in matured vitro to a stable "developed" state rate similar...
General anesthetics induce loss of consciousness, a global change in behavior. However, corresponding activity the context defined cortical cell types has not been identified. Here, we show that spontaneous mouse layer 5 pyramidal neurons, but no other type, becomes consistently synchronized vivo by different general anesthetics. This heightened neuronal synchrony is aperiodic, present across large distances, and absent neurons presynaptic to neurons. During transition from anesthesia,...
Cortical circuits are composed predominantly of pyramidal-to-pyramidal neuron connections, yet their assembly during embryonic development is not well understood. We show that mouse Rbp4-Cre cortical neurons, transcriptomically closest to layer 5 pyramidal display two phases circuit in vivo. At E14.5, they form a multi-layered motif, only near-projecting-type neurons. By E17.5, this transitions second motif involving all three types, analogous the adult types. In vivo patch clamp recordings...
We examined how correlated firing controls axon remodeling, using in vivo time-lapse imaging and electrophysiological analysis of individual retinal ganglion cell (RGC) axons that were visually stimulated either synchronously or asynchronously relative to neighboring inputs the Xenopus laevis optic tectum. RGCs out synchrony rapidly lost ability drive tectal postsynaptic partners while their grew added many new branches. In contrast, activated produced fewer branches, but these more stable....
Although somatosensation in multiple whisker systems has been studied considerable detail, relatively little information is available regarding usage and movement patterns during natural behaviors. The Etruscan shrew, one of the smallest mammals, relies heavily on its system to detect kill highly mobile insect prey. Here, we tracked body motion prey capture. We found that shrews made periodic movements (whisking) with frequencies ranging from 12 17 Hz. compared shrew rat whisking was smaller...
In a small fraction of Xenopus tadpoles, single retinal ganglion cell (RGC) axon misprojects to the ipsilateral optic tectum. Presenting flashes light eye causes that fire, whereas stimulating contralateral excites all other RGC inputs We performed time-lapse imaging individual ipsilaterally projecting axons while either or eye. Stimulating alone reduced elaboration by increasing branch loss. New additions in ipsi were exclusively increased stimulation, which was enhanced expressing tetanus...
Summary How closely human organoids recapitulate cell-type diversity and maturation of their target organs is not well understood. We developed retinal with multiple nuclear synaptic layers. sequenced the RNA 158,844 single cells from these at six developmental time points periphery, fovea, pigment epithelium choroid light-responsive adult retinas, performed histochemistry. Cell types in matured vitro to a stable ‘developed’ state rate similar retina development vivo transcriptomes organoid...
During development, patterned neural activity instructs topographic map refinement. Axons with similar patterns of converge onto target neurons and stabilize their synapses these postsynaptic partners, restricting exploratory branch elaboration (Hebbian structural plasticity). On the other hand, non-correlated firing in inputs leads to synapse weakening increased growth axons (Stentian We used visual stimulation control correlation structure a few ipsilaterally projecting (ipsi) retinal...
How closely human organoids recapitulate cell-type diversity and maturation of their target organs is not well understood. We developed retinal with multiple nuclear synaptic layers. sequenced the RNA 158,844 single cells from these at six developmental time points periphery, fovea, pigment epithelium choroid light-responsive adult retinas, performed histochemistry. Cell types in matured vitro to a stable 'developed' state rate similar retina development vivo transcriptomes organoid cell...
Summary Cortical circuits are composed predominantly of pyramidal-to-pyramidal neuron connections, yet their assembly during embryonic development is not well understood. We show that layer 5 pyramidal neurons, identified through single cell transcriptomics, display two phases circuit in vivo. At E14.5, a multi-layered motif, type, forms. This motif transient, switching to second involving all three types, by E17.5. In vivo targeted recordings and two-photon calcium imaging neurons reveal...
Abstract During development, patterned neural activity instructs topographic map refinement. Axons with similar patterns of activity, converge onto target neurons and stabilize their synapses these postsynaptic partners, restricting exploratory branch elaboration (Hebbian structural plasticity). On the other hand, non-correlated firing in inputs leads to synapse weakening increased growth axons (Stentian We used visual stimulation control correlation structure a few ipsilaterally projecting...