A5004084769- Retinal Diseases and Treatments
- Glaucoma and retinal disorders
- Retinal Imaging and Analysis
- Intraocular Surgery and Lenses
- Optical Coherence Tomography Applications
- Retinal Development and Disorders
- Retinal and Optic Conditions
- Retinal and Macular Surgery
- Corneal surgery and disorders
- Ocular Diseases and Behçet’s Syndrome
- Corneal Surgery and Treatments
- Cerebral Venous Sinus Thrombosis
- Ocular Surface and Contact Lens
- RNA regulation and disease
- Ocular Infections and Treatments
- Traumatic Ocular and Foreign Body Injuries
- Ophthalmology and Visual Impairment Studies
- Mycobacterium research and diagnosis
- Ocular Oncology and Treatments
- Vascular Malformations Diagnosis and Treatment
- Ocular Disorders and Treatments
- Ocular and Laser Science Research
- Ophthalmology and Eye Disorders
- Single-cell and spatial transcriptomics
- Orthopedic Surgery and Rehabilitation
University of Basel
2015-2025
University Hospital of Basel
2014-2024
Institute of Molecular and Clinical Ophthalmology Basel
2019-2024
Kantonsspital Aarau
2019
Glostrup Hospital
2007-2010
University of Copenhagen
2007-2010
University Hospital of Bern
2009
Human organoids recapitulating the cell-type diversity and function of their target organ are valuable for basic translational research. We developed light-sensitive human retinal with multiple nuclear synaptic layers functional synapses. sequenced RNA 285,441 single cells from these at seven developmental time points periphery, fovea, pigment epithelium choroid light-responsive adult retinas, performed histochemistry. Cell types in matured vitro to a stable "developed" state rate similar...
Purpose To benchmark the human and machine performance of spectral-domain (SD) swept-source (SS) optical coherence tomography (OCT) image segmentation, i.e., pixel-wise classification, for compartments vitreous, retina, choroid, sclera. Methods A convolutional neural network (CNN) was trained on OCT B-scan images annotated by a senior ground truth expert retina specialist to segment posterior eye compartments. Independent data sets (30 SDOCT 30 SSOCT) were manually segmented three classes...
Purpose: To study a novel and fast optical coherence tomography (OCT) device for home-based monitoring in age-related macular degeneration (AMD) small sample yielding sparse OCT (spOCT) data to compare the commercially available reference device. Methods: In this prospective study, both eyes of 31 participants with AMD were included. The subjects underwent scanning an prototype spectral-domain accuracy central retinal thickness (CRT) measurements. Results: Sixty-two (21 females 10 males)...
Stargardt disease is a currently untreatable, inherited neurodegenerative that leads to macular degeneration and blindness due loss-of-function mutations in the ABCA4 gene. We have designed dual adeno-associated viral vector encoding split-intein adenine base editor correct most common mutation (c.5882G>A, p.Gly1961Glu). optimized editing human models, including retinal organoids, induced pluripotent stem cell-derived pigment epithelial (RPE) cells, as well adult explants RPE/choroid vitro....
Abstract Background Loss of corneal endothelial cells after glaucoma surgery can lead to decompensation and reduced vision. This loss may be accelerated by drainage implants like PreserFlo, which allow controlled subconjunctival filtration. In a retrospective analysis, we examined its impact on cell density (ECD). Patients methods Data from medical records for 94 eyes were retrospectively analyzed. included 47 PreserFlo-operated contralateral control eyes. Inclusion criteria open-angle...
Abstract Purpose To evaluate and to compare the safety of intravitreal ranibizumab injections performed by physicians nurses at a single large hospital clinic in D enmark during 5 years. Design Retrospective, interventional, non‐comparative study. Methods Setting : All eyes that underwent protocolized injection procedure an operating room mainly their first year ophthalmology training. Study population A total 4623 3679 patients with subretinal neovascularization secondary variety retinal...
To study the relationship between intraretinal optical coherence tomography (OCT) and fluorescein angiography (FA) findings in eyes with diabetic macular oedema (DMO).We carried out a retrospective observational case series. Thirty previously untreated DMO underwent FA OCT. The same ETDRS template was overlaid on images order to compare OCT FA. Transfoveal linear high-resolution scans (at 0- 90-degree meridians) pictures were compared according rings.Six distinct patterns of changes...
Feasibility testing of a novel volume renders technology to display optical coherence tomography data (OCT) in virtual reality (VR) environment.A VR program was written C++/OpenGL import and volumetric OCT real time with 180 frames per second using high-end computer tethered head-mounted display. Following exposure, participants completed Simulator Sickness Questionnaire (SSQ) assess for nausea, disorientation, oculomotor disturbances. A user evaluation study this software conducted explore...
Abstract Machine learning has greatly facilitated the analysis of medical data, while internal operations usually remain intransparent. To better comprehend these opaque procedures, a convolutional neural network for optical coherence tomography image segmentation was enhanced with Traceable Relevance Explainability (T-REX) technique. The proposed application based on three components: ground truth generation by multiple graders, calculation Hamming distances among graders and machine...
ABSTRACT Stargardt disease is a currently untreatable, inherited neurodegenerative that leads to macular degeneration and blindness due loss-of-function mutations in the ABCA4 gene. We have designed dual adeno-associated viral vector split-intein adenine base-editing strategy correct most common mutation (c.5882G>A, p.G1961E). optimized base editing human models, including retinal organoids, iPSC-derived pigment epithelial (RPE) cells, as well adult retinal- RPE/choroid explants vitro....
To assess the impact of vascular dysregulation on choroidal blood flow response to hand-grip test and relevance this glaucoma.Eighty healthy volunteers underwent a while was measured by means laser Doppler flowmetry. Blood pressure, heart rate, intraocular pressure (IOP) were monitored. Choroidal changes compared between subjects with positive history cold hands control analysis variance. The relationship level IOP at which progressive damage occurred analyzed in 21 patients primary...
<h3>Aims:</h3> To evaluate morphological and functional recovery after macular hole surgery using pars plana vitrectomy (PPV), inner limiting membrane (ILM) peeling, air-tamponade short-time limited face-down positioning. <h3>Design:</h3> Retrospective, interventional, non-comparative consecutive case series. <h3>Patients:</h3> Thirty eyes in 30 patients (30 eyes) with unilateral full-thickness (stage II–IV). <h3>Intervention:</h3> All underwent PPV, ILM peeling fluid–air exchange followed...
To study microperimetric macular sensitivity in diabetic oedema (DMO) relation to lesion characteristics obtained by optical coherence tomography (OCT), colour fundus photography, and fluorescein angiography (FA).The comprised 20 eyes 15 patients with nonproliferative retinopathy recently diagnosed untreated DMO. Investigations included microperimetry, angiography, OCT. All measures gradings were made for each of the nine fields an early treatment macula template. Statistical analysis was...
The choroid has the highest blood flow of any structure in human body with specific hemodynamic regulatory mechanisms that differ from those retinal circulation. It is modulated by a strong autonomic input and largely insensitive to light stimulation differences oxygenation (Kur et al. 2012). Detailed imaging choroidal layers recently been facilitated introduction new modalities such as enhanced depth optical coherence tomography (EDI-OCT) swept source OCT (SSOCT) (Mrejen & Spaide 2013). One...
Summary How closely human organoids recapitulate cell-type diversity and maturation of their target organs is not well understood. We developed retinal with multiple nuclear synaptic layers. sequenced the RNA 158,844 single cells from these at six developmental time points periphery, fovea, pigment epithelium choroid light-responsive adult retinas, performed histochemistry. Cell types in matured vitro to a stable ‘developed’ state rate similar retina development vivo transcriptomes organoid...
<h3>Objective</h3> To assess the relationship between ocular perfusion pressure and blood flow in choroid subjects with acral vasospasm. <h3>Patients Methods</h3> Twenty otherwise healthy vascular dysregulation 55 age-matched nonvasospastic volunteers were recruited. After a 20-minute rest sitting position, intraocular choroidal determined by means of applanation tonometry laser Doppler flowmetry, respectively. The flowmetry variables velocity, volume, flux assessed. correlations mean ({⅔ ×...
Volume rendering of optical coherence tomography angiography (OCTA) is a rapidly evolving imaging tool, which has been shown to preserve the three-dimensional (3D) architecture various retinal diseases including diabetic retinopathy, vein occlusion and macular telangiectasia type 2 (Spaide 2015). This form avoids flattening subvolumes tissue as done in en face does not require use segmentation, often incorrect disease. can illustrate close relationship between flow signal structural (OCT)...
Optical coherence tomography (OCT) is a high-resolution noninvasive technology used in medical imaging for the spatial visualization of biological tissue. Due to its coherent nature, OCT suffers from speckle noise, which significantly degrades information content resulting scans. We introduce new filtering method three-dimensional images, inspired by film grain removal techniques. By matching structural relatedness along all dimensions, algorithm builds up vector paths every voxel image...
There is increased interest to use minipigs in ocular toxicology studies due their anatomical similarities with human eyes and as a substitute for nonhuman primates. This requires adaptation of enhanced optical coherence tomography (OCT) techniques relevant immunohistochemistry (IHC) or situ hybridization (ISH) markers porcine eyes. In this study, OCT angiography (AngioOCT) were performed on adult Göttingen minipigs. To increase structural information retinal choroidal vasculature, data...
To explore morphologic characteristics of choroidal lesions in patients with disseminated Mycobacterium chimaera infection subsequent to open-heart surgery.Nine (18 eyes) systemic M. were reviewed. Activity evaluated using biomicroscopy, fundus autofluorescence, enhanced depth imaging optical coherence tomography, fluorescein angiography/indocyanine green angiography, and tomography angiography. Relationships findings disease activity sought.All 9 male patients, aged between 49 66 years,...
How closely human organoids recapitulate cell-type diversity and maturation of their target organs is not well understood. We developed retinal with multiple nuclear synaptic layers. sequenced the RNA 158,844 single cells from these at six developmental time points periphery, fovea, pigment epithelium choroid light-responsive adult retinas, performed histochemistry. Cell types in matured vitro to a stable 'developed' state rate similar retina development vivo transcriptomes organoid cell...
To evaluate the clinical outcome of subretinal autologous internal limiting membrane (ILM) transplantation during pars-plana vitrectomy for persistent full-thickness macular hole (FTMH) repair. Retrospective, consecutive case series 13 eyes (13 patients) undergoing small-incision with ILM and air tamponade large FTMH after prior unsuccessful posterior hyaloid detachment peeling. For all eyes, high-definition spectral domain optical coherence tomography scans (SD-OCT Spectralis, Heidelberg...