A5043739803- Electrochemical sensors and biosensors
- Microbial Metabolic Engineering and Bioproduction
- Metabolism and Genetic Disorders
- Amino Acid Enzymes and Metabolism
- Parkinson's Disease Mechanisms and Treatments
- Mitochondrial Function and Pathology
- Biochemical Acid Research Studies
- Biochemical effects in animals
- Coenzyme Q10 studies and effects
- Photosynthetic Processes and Mechanisms
- Muscle metabolism and nutrition
- Molecular Sensors and Ion Detection
- Enzyme Catalysis and Immobilization
- Monoclonal and Polyclonal Antibodies Research
- Neuroscience and Neuropharmacology Research
- Cardiac electrophysiology and arrhythmias
- Enzyme function and inhibition
- Biotin and Related Studies
- Biochemical Analysis and Sensing Techniques
- Ion channel regulation and function
- Analytical Chemistry and Chromatography
- Neurological disorders and treatments
- Adipose Tissue and Metabolism
- Pharmacogenetics and Drug Metabolism
- Meat and Animal Product Quality
University of Bern
2020-2024
Oeschger Centre for Climate Change Research
2020-2024
Paul Scherrer Institute
2020-2024
Roche (Switzerland)
2013-2022
TU Dresden
2016-2020
Sinclair Community College
2020
First Technical University
2020
Tennessee Technological University
2020
Community College of Philadelphia
2020
University of Louisville Hospital
2020
MPTP, l-methyl-4-phenyl-l,2,3,6tetrahydropyridine; MPP+, 1-methyl-4-phenylpyridinium ion; MPDP+, l-methyl-4-phenyl-2,3,-dihydropyridinium MOPS, 3-(N-morpho1ino)propanesulfonic acid; EGTA, [ethylenebis
Modeling clinically relevant tissue responses using cell models poses a significant challenge for drug development, in particular induced liver injury (DILI). This is mainly because existing lack longevity and tissue-level complexity which limits their utility predictive toxicology. In this study, we established characterized novel bioprinted human mimetics comprised of patient-derived hepatocytes non-parenchymal cells defined architecture. Scaffold-free assembly different types an...
This chapter contains sections titled: Introduction Assay of Succinate Dehydrogenase and Succinoxidase in Animal Tissues Application Assays to Yeast, Bacteria, Higher Plans NADH Oxidase Choline Mitochondrial α-Glycerophosphate
Dilute solutions of sulfhydryl enzymes (phosphoglyceraldehyde dehydrogenase, adenosinetriphosphatase, succinoxidase) showed reduced activity on irradiation by small amounts x-rays. When the inhibition was partial enzyme reactivated addition glutathione. more complete, reactivation only partial. These observations are interpreted as being due to oxidation -SH groups protein products water irradiation, radicals OH and O(2)H, H(2)O(2) atomic oxygen. The irreversible which occurs when dose...
Abstract: Nigrostriatal cell death in 1‐methyl‐4‐phenyl‐1,2,3,6‐tetrahydropyridine (MPTP)‐induced Parkinson's disease results from the inhibition of mitochondrial respiration by 1‐methyl‐4‐phenylpyridinium (MPP + ). MPP blocks electron flow NADH dehydrogenase to coenzyme Q at or near same site as do rotenone and piericidin protects against binding loss activity due these inhibitors. The 4′‐analogs showed increasing affinity for with length alkyl chain, lowest K i , 4′‐heptyl‐MPP being 6 μ M...
A method has been elaborated for the large scale isolation of outer membranes beef liver mitochondria. From these monoamine oxidase was partially purified and digested with trypsin chymotrypsin to yield peptides containing riboflavin covalently linked peptide chain. pure pentapeptide riboflavin‐5′‐phosphate obtained by a series chromatographic procedures. Edman degradation, followed dansylation, revealed amino acid sequence: Ser‐Gly‐Gly‐X‐Tyr, X being which flavin is attached via 8α‐carbon...
The binding sites of rotenone, piericidin A, and Amytal in the reduced nicotinamide adenine dinucleotide oxidase chain heart have been studied with aid rotenone-6a-14C. Binding rotenone continues a linear manner beyond point maximal inhibition respiration, indicating that is tightly bound not only at specific site NADH dehydrogenase segment respiratory but also other submitochondrial particles. Unspecific by treatment particles presence bovine serum albumin (BSA) further minimized successive...
This chapter contains sections titled: Introduction Assay of the Primary Dehydrogenase Succinic Complex
Abstract On the addition of NADH to submitochondrial particles in which oxidase is blocked by rotenone, piericidin A, or Amytal, g = 1.94 signal dehydrogenase appears essentially same manner as untreated preparations. However, appearance NADHinduced iron succinate and cytochromes b c1 inhibited these agents. It concluded that block oxidation on O2 side non-heme dehydrogenase.