A5051016750- Retinal Development and Disorders
- Neural dynamics and brain function
- Neuroscience and Neural Engineering
- Cell Image Analysis Techniques
- Advanced Fluorescence Microscopy Techniques
- EEG and Brain-Computer Interfaces
Institute of Molecular and Clinical Ophthalmology Basel
2020-2022
Human organoids recapitulating the cell-type diversity and function of their target organ are valuable for basic translational research. We developed light-sensitive human retinal with multiple nuclear synaptic layers functional synapses. sequenced RNA 285,441 single cells from these at seven developmental time points periphery, fovea, pigment epithelium choroid light-responsive adult retinas, performed histochemistry. Cell types in matured vitro to a stable "developed" state rate similar...
Abstract Neural information processing requires accurately timed action potentials arriving from presynaptic neurons at the postsynaptic neuron. However, axons of ganglion cells in human retina feature low axonal conduction speeds and vastly different lengths, which poses a challenge to brain for constructing temporally coherent image over visual field. Combining results microelectrode array recordings, behavioral measurements, transmission electron microscopy, mathematical modelling retinal...
Timing between action potentials is crucial for information processing in real neural networks. However, the physical axonal lengths largely determine time necessary to reach postsynaptic neurons. Retinal ganglion cell (RGC) axons form retinal nerve fiber layer (RNFL). The human RNFL shows a highly stereotypical organization characterized by presence of fovea, specialized region enabling high-resolution vision tasks, such as reading. To papilla (i.e., optic head), do not cross but rather...