A5019197062- Botanical Research and Applications
- Genetics, Aging, and Longevity in Model Organisms
- RNA Research and Splicing
- Plant Diversity and Evolution
- Bone health and osteoporosis research
- CRISPR and Genetic Engineering
- Plant tissue culture and regeneration
- Genomics and Chromatin Dynamics
- RNA and protein synthesis mechanisms
- Cassava research and cyanide
- Photosynthetic Processes and Mechanisms
- Body Composition Measurement Techniques
- Nutrition and Health in Aging
- Plant Physiology and Cultivation Studies
- Chromosomal and Genetic Variations
- Ubiquitin and proteasome pathways
- Polysaccharides and Plant Cell Walls
- Postharvest Quality and Shelf Life Management
- Plant Taxonomy and Phylogenetics
- Lysosomal Storage Disorders Research
- Cellular transport and secretion
- Catalytic Alkyne Reactions
- Advanced biosensing and bioanalysis techniques
- Genomics, phytochemicals, and oxidative stress
- Plant Molecular Biology Research
South China Agricultural University
2022-2025
Ministry of Agriculture and Rural Affairs
2022-2025
Institute of Molecular Biology
2020-2025
Zhejiang University-University of Edinburgh Institute
2024
Max Delbrück Center
2010-2023
Cancer Research UK
2023
University of Cambridge
2019-2023
Johannes Gutenberg University Mainz
2023
National Tsing Hua University
2020-2021
Cancer Research UK Cambridge Center
2019
Stable isotope labeling by amino acids in cell culture (SILAC) is widely used to quantify protein abundance tissue cells. Until now, the only multicellular organism completely labeled at acid level was laboratory mouse. The fruit fly Drosophila melanogaster one of most small animal models biology. Here, we show that feeding flies with SILAC-labeled yeast leads almost complete first filial generation. We these "SILAC flies" investigate sexual dimorphism D. melanogaster. Quantitative proteome...
Centrioles are core structural elements of both centrosomes and cilia. Although cytoplasmic granules called centriolar satellites have been observed around these structures, lack a comprehensive inventory satellite proteins impedes our understanding their ancestry. To address this, we performed mass spectrometry (MS)-based proteome profiling obtained by affinity purification key constituent, PCM1, from sucrose gradient fractions. We defined an interactome consisting 223 proteins, which...
Pitaya (Selenicereus) is a kind of novel fruit with delicious taste and superior horticulture ornamental value. The potential economic impact the pitaya lies in its diverse uses not only as agricultural produce processed foods but also industrial medicinal products. It an excellent plant material for basic applied biological research. A comprehensive database would facilitate studies other Cactaceae species. Here, we constructed genome multiomics database, which collection most updated...
The nucleosomal landscape of chromatin depends on the concerted action remodelers. INO80 remodeler specifically places nucleosomes at boundary gene regulatory elements, which is proposed to be result an ATP-dependent nucleosome sliding activity that regulated by extranucleosomal DNA features. Here, we use cryo-electron microscopy and functional assays reveal how binds DNA. Structures A-module bound clarify mechanism linker binding. connected motor unit via HSA/post-HSA lever element...
Reactive aldehydes are produced by normal cellular metabolism or after alcohol consumption, and they accumulate in human tissues if aldehyde clearance mechanisms impaired. Their toxicity has been attributed to the damage cause genomic DNA subsequent inhibition of transcription replication. However, whether interference with other processes contributes not investigated. We demonstrate that formaldehyde induces RNA-protein crosslinks (RPCs) stall ribosome inhibit translation cells. RPCs...
Abstract The ability to assign cellular origin low-abundance secreted factors in extracellular vesicles (EVs) would greatly facilitate the analysis of paracrine-mediated signaling. Here, we report a method, named selective isolation (SIEVE), which uses cell type-specific proteome labeling via stochastic orthogonal recoding translation (SORT) install bioorthogonal reactive groups into proteins derived from cells targeted for labeling. We establish native purification intact EVs target cell,...
Multiple MYB transcription factors (TFs) are involved in the regulation of plant coloring. Betalain is a kind natural pigment and its biosynthesis regulated by number enzymes. Despite this, little known about molecular properties roles TFs pitaya betalain biosynthesis.In present study, we identified 1R-MYB gene, HuMYB132, which preferentially expressed red-pulp at mature stage. It was clustered with Arabidopsis R-R-type genes had two DNA-binding domains histidine-rich region. The expression...
Pitaya canker disease, caused by Neoscytalidium dimidiatum, is the primary threat to pitaya cultivation, significantly compromising fruit quality and reducing yield. WRKY transcription factors are essential regulators in plant pathogen recognition defense mechanisms, yet their specific roles development of disease remain largely unexplored. In this study, five genes (HmeWRKY33, HmeWRKY51, HmePR1, HmeHsp70, HmeSERK) associated with were identified through RNA-Seq analysis. The expression...
Flower induction in pitaya (Hylocereus polyrhizus) is regulated by complex gene networks involving multiple signaling pathways that ensure flower bud (FB) formation, but its molecular determinants remain largely unknown. In this study, we aimed to identify key genes and involved analyzing transcriptomics profiles from differentiating buds. Our results indicate the process driven a combination of sugar, hormone, transcription factor (TF), flowering-related genes. We found during FB period,...
Summary The MRE11-RAD50-NBS1 (MRN) complex is a central, multifunctional factor in the detection, signaling and nucleolytic processing of DNA double-strand breaks (DSBs). To clarify how human MRN binds generic telomeric ends can separate end sensing from nuclease activities, we determined cryo-electron microscopy structures bound to telomere protection TRF2. senses DSBs through tight clamp-like state with closed coiled-coil domains, but auto-inhibited MRE11 nuclease. NBS1 wraps around dimer,...
<title>Abstract</title> Intergeneric hybridization and domestication breeding is an efficient means for utilizing wild rice germplasm resources. In this study, we constructed gap-less genome assemblies two CC, BBCC one CCDD rices to explore the role of C subgenome in diversification. Furthermore, assembled a cultivated (AA) further developed A/C hybrids with enhanced resistance BPH using hybrid embryo rescue system. To facilitate tracking chromosomes backcross selfed progeny these hybrids,...
This work reports gold-catalyzed highly enantioselective nitroso-Povarov reactions between cyclopentadienes and nitrosoarenes in cold dichloroethane, which serve as 4π-electron donors 2π-donors. High enantioselectivity has been achieved for substrates over a wide scope. With the same chiral catalyst, nitroso-4-fluorobenzenes these under DCM/THF/water/air led to oxidative also with high enantioselectivity. We performed 18O-labeling experiments confirm that both water O2 participate reactions;...
Transposons are discrete segments of DNA that have the distinctive ability to move and replicate within genomes across tree life. 'Cut paste' transposition involves excision from a donor locus reintegration into new in genome. We studied molecular events following steps two eukaryotic transposons, Sleeping Beauty (SB) piggyBac (PB) widely used for genome manipulation vertebrate species. SB originates fish PB insects; thus, by introducing these transposons human cells we aimed monitor process...
Eukaryotic gene expression requires the coordinated action of transcription factors, chromatin remodelling complexes and RNA polymerase. The conserved nuclear protein Akirin plays a central role in immune insects mammals, linking SWI/SNF chromatin-remodelling complex with factor NFκB. Although nematodes lack NFκB, is also indispensable for defence genes epidermis Caenorhabditis elegans following natural fungal infection. Through combination reverse genetics biochemistry, we discovered that...
Abstract Genetic variants of GBA1 can cause the lysosomal storage disorder Gaucher disease and are among highest genetic risk factors for Parkinson's (PD). encodes enzyme beta‐glucocerebrosidase (GCase), which orchestrates degradation glucosylceramide (GluCer) in lysosome. Recent studies have shown that GluCer accelerates α ‐synuclein aggregation, exposing GCase deficiency as a major factor PD pathology promising target treatment. This study investigates interaction three disease‐associated...
Studying protein interactions in whole organisms is fundamental to understanding development. Here, we combine vivo expressed GFP-tagged proteins with quantitative proteomics identify protein-protein of selected key involved early C. elegans embryogenesis. Co-affinity purification interaction partners for eight bait resulted a pilot map focus on Our network reflects known biology and highly enriched functionally relevant interactions. To demonstrate the utility map, looked new regulators P...