A5088642562- Neurobiology and Insect Physiology Research
- Advanced Fluorescence Microscopy Techniques
- Genomics and Chromatin Dynamics
- Mesoporous Materials and Catalysis
- Porphyrin and Phthalocyanine Chemistry
- Photochemistry and Electron Transfer Studies
- DNA and Nucleic Acid Chemistry
- Molecular Junctions and Nanostructures
- RNA and protein synthesis mechanisms
- Advanced biosensing and bioanalysis techniques
- Luminescence and Fluorescent Materials
- Barrier Structure and Function Studies
- Advanced Electron Microscopy Techniques and Applications
- Photosynthetic Processes and Mechanisms
- Immune Response and Inflammation
- Developmental Biology and Gene Regulation
- Erythrocyte Function and Pathophysiology
- Gold and Silver Nanoparticles Synthesis and Applications
- Zeolite Catalysis and Synthesis
- RNA Research and Splicing
- Single-cell and spatial transcriptomics
- Viral Infectious Diseases and Gene Expression in Insects
- Microfluidic and Capillary Electrophoresis Applications
- bioluminescence and chemiluminescence research
- Nanopore and Nanochannel Transport Studies
Ludwig-Maximilians-Universität München
2015-2025
Center for Integrated Protein Science Munich
2009-2022
Center for NanoScience
2007-2011
Max Planck Institute for Polymer Research
2006-2008
University of Bremen
2007
Nanosystems Initiative Munich
2007
University of Illinois Urbana-Champaign
2006
Glia play crucial roles in the development and homeostasis of nervous system. While GLIA Drosophila embryo have been well characterized, their study adult system has limited. Here, we present a detailed description glia system, based on analysis some 500 glial drivers identified within collection synthetic GAL4 lines. We find that make up ∼10% cells envelop all compartments neurons (soma, dendrites, axons) as whole. Our morphological suggests set simple rules governing morphogenesis...
Toll-like receptor 7 (TLR7) is essential for recognition of RNA viruses and initiation antiviral immunity. TLR7 contains two ligand-binding pockets that recognize different degradation products: pocket 1 recognizes guanosine, while 2 coordinates pyrimidine-rich fragments. We found the endonuclease RNase T2, along with 5′ exonucleases PLD3 PLD4, collaboratively generate ligands TLR7. Specifically, T2 generated guanosine 2′,3′-cyclic monophosphate-terminated PLD exonuclease activity further...
Article25 August 2020Open Access Source DataTransparent process CARD8 inflammasome activation triggers pyroptosis in human T cells Andreas Linder Gene Center and Department of Biochemistry, Ludwig-Maximilians-Universität München, Munich, Germany Medicine II, University Hospital, Search for more papers by this author Stefan Bauernfried Yiming Cheng Manuel Albanese Max von Pettenkofer Institute, Virology, Christophe Jung Oliver Keppler Veit Hornung Corresponding Author [email protected]...
A new terrylene diimide-based dye (WS-TDI) that is soluble in water has been synthesized, and its photophysical properties are characterized. WS-TDI forms nonfluorescing H-aggregates show absorption bands being blue-shifted with respect to those of the fluorescing monomeric form. The ratio aggregated was determined be 1 14 400 from fluorescence correlation spectroscopy (FCS) measurements, suggesting presence a large amount soluble, nonfluorescent aggregates water. surfactant such as Pluronic...
We apply mesoporous thin silica films with nanometer-sized pores as drug carriers and incorporate the widely used anticancer Doxorubicin. Through single-molecule based measurements, we gain mechanistic insights into diffusion inside film, which governs drug-delivery at target-site. Drug dynamics nanopores is controlled by pore size surface modification. The release kinetics determined live-cell measurements prove applicability of system for drug-delivery. This study demonstrates that...
Single dye molecules incorporated into a mesoporous matrix can act as highly sensitive reporters of their environment. Here, we use single TDI guests hexagonal films containing structured domains. The allow us to map the size these domains which extend over 100 μm. Investigation translational and orientational dynamics via molecule fluorescence techniques gives structural well dynamical information about host material. In an air atmosphere, guest show no movement but perfect orientation...
Summary The MRE11-RAD50-NBS1 (MRN) complex is a central, multifunctional factor in the detection, signaling and nucleolytic processing of DNA double-strand breaks (DSBs). To clarify how human MRN binds generic telomeric ends can separate end sensing from nuclease activities, we determined cryo-electron microscopy structures bound to telomere protection TRF2. senses DSBs through tight clamp-like state with closed coiled-coil domains, but auto-inhibited MRE11 nuclease. NBS1 wraps around dimer,...
The core promoter plays a central role in setting metazoan gene expression levels, but how exactly it "computes" remains poorly understood. To dissect its function, we carried out comprehensive structure-function analysis Drosophila. First, performed genome-wide bioinformatic analysis, providing an improved picture of the sequence motifs architecture. We then measured synthetic promoters' activities ~3,000 mutational variants with and without external stimulus (hormonal activation), at large...
Nanostructured host−guest materials are important for various applications in nanoscience, and therefore, a thorough understanding of the dynamics guest molecules within host matrix is needed. To this aim we used single-molecule fluorescence techniques to simultaneously examine spectral orientational behavior single nanostructured porous materials. Two types systems have been investigated. First, oxazine-1 dye were fixed rigidly channels microporous AlPO4-5 crystals. Second, it was shown...
The complex patterns of gene expression in metazoans are controlled by selective binding transcription factors (TFs) to regulatory DNA. To improve the quantitative understanding this process, we have developed a novel method that uses fluorescence anisotropy measurements delivery system determine TF-DNA energies solution with high sensitivity and throughput. Owing its large dynamic range, method, named performance (HiP-FA), allows for reliable quantification both weak strong binding;...
Abstract The photophysical properties of three new water‐soluble terrylenediimide (WS‐TDI) derivatives are investigated and their utilization in biological experiments is demonstrated. Each these dyes can be excited the far red region visible spectrum, making them good candidates for in‐vivo studies. Single‐molecule techniques characterize photophysics that is, number emitted photons, blinking characteristics survival times until photobleaching takes place. All exhibit bright fluorescence,...
Necroptosis is a form of regulated cell death that can occur downstream several immune pathways. While previous studies have shown dysregulated necroptosis lead to strong inflammatory responses, little known about the identity endogenous molecules trigger these responses. Using reductionist in vitro model, we found soluble TNF strongly released context necroptosis. On one hand, promotes translation by inhibiting negative regulatory mechanisms acting at post-transcriptional level. other...
The blood–brain barrier (BBB) of Drosophila comprises a thin epithelial layer subperineural glia (SPG), which ensheath the nerve cord and insulate it against potassium-rich hemolymph by forming intercellular septate junctions (SJs). Previously, we identified novel Gi/Go protein-coupled receptor (GPCR), Moody, as key factor in BBB formation at embryonic stage. However, molecular cellular mechanisms Moody signaling maturation remain unclear. Here, identify cAMP-dependent protein kinase A (PKA)...
The translational and orientational movement of single terrylenediimide dye molecules incorporated as guests in the nanometer-sized pores an M41S host silica film is observed simultaneously (see figure). By correlating dynamics position orientation well emission spectra molecules, nanoscaled heterogeneities mesoporous are determined.
Abstract Three new photostable rylene dyes for applications in single molecule studies and membrane labelling have been synthesized their photophysical properties were characterized. These differ the number of polyethylene glycol (PEG) chains attached to core structure which is either a perylene derivate or terrylene derivate. One one dye modified with two PEG chains, another has four chains. The results show that (4‐PEG‐TDI) forms soluble nonfluorescing H‐aggregates water, so absorption...
Epithelial sheets play essential roles as selective barriers insulating the body from environment and establishing distinct chemical compartments within it. In invertebrate epithelia, septate junctions (SJs) consist of large multi-protein complexes that localize at apicolateral membrane mediate barrier function. Here, we report identification two novel SJ components, Pasiflora1 Pasiflora2, through a genome-wide glial RNAi screen in Drosophila. Pasiflora mutants show permeable blood-brain...
An essential event in gene regulation is the binding of a transcription factor (TF) to its target DNA. Models considering interactions between TF and DNA geometry proved be successful approaches describe this event, while conserving data interpretability. However, direct characterization shape contribution still missing due lack accurate large-scale affinity data. Here, we use assay recently established measure with high sensitivity specificities 13 Drosophila TFs, including dinucleotide...
We investigated silver nanowires using correlative wide-field fluorescence and transmission electron microscopy. In the images, synchronous emission from different distinct positions along was observed. The sites of were separated spatially by up to several micrometers. Nanowires emitting in such cooperative manner then also with a combination microscopy based techniques, as high-resolution, bright-field imaging, diffraction, high-angle annular dark-field energy-dispersive X-ray...
Fluorescent tagging of viral particles by genetic means enables the study virus dynamics in living cells. However, beta-herpesvirus entry and morphogenesis this method is currently limited. This due to lack replication competent, capsid-tagged fluorescent viruses. Here, we report on viable recombinant MCMVs carrying ectopic insertions small capsid protein (SCP) fused proteins (FPs). The FPs were inserted into an internal position which allowed production viable, fluorescently labeled...