A5066218755- Cell Image Analysis Techniques
- Advanced Fluorescence Microscopy Techniques
- Protein Kinase Regulation and GTPase Signaling
- Plant nutrient uptake and metabolism
- Genomics and Chromatin Dynamics
- Biocrusts and Microbial Ecology
- Cellular Mechanics and Interactions
- Greenhouse Technology and Climate Control
- Plant responses to water stress
- bioluminescence and chemiluminescence research
- Health and Medical Research Impacts
- Photosynthetic Processes and Mechanisms
- Electron and X-Ray Spectroscopy Techniques
- Signaling Pathways in Disease
- Single-cell and spatial transcriptomics
- Scientific Computing and Data Management
- Plant Molecular Biology Research
- Cellular transport and secretion
- Global Health and Surgery
- Advanced Electron Microscopy Techniques and Applications
- Tree Root and Stability Studies
- Advanced biosensing and bioanalysis techniques
- Polysaccharides and Plant Cell Walls
- Molecular Biology Techniques and Applications
- RNA Research and Splicing
European Molecular Biology Laboratory
2006-2024
German BioImaging – Gesellschaft für Mikroskopie und Bildanalyse
2020
European Bioinformatics Institute
2009
Forschungszentrum Jülich
2009
European Molecular Biology Organization
2009
Heidelberg University
2001-2002
Heidelberg University
2001
Root phenotyping is a challenging task, mainly because of the hidden nature this organ. Only recently, imaging technologies have become available that allow us to elucidate dynamic establishment root structure and function in soil. In tips, optical analysis relative elemental growth rates expansion zones hydroponically-grown plants revealed it maximum intensity cellular processes rather than length zone control acclimation changes temperature. Acclimation entire systems was studied at high...
Abstract In the dynamic landscape of scientific research, imaging core facilities are vital hubs propelling collaboration and innovation at technology development dissemination frontier. Here, we present a collaborative effort led by Global BioImaging (GBI), introducing international recommendations geared towards elevating careers Imaging Scientists in facilities. Despite critical role modern research ecosystems, challenges persist recognising their value, aligning performance metrics...
A newly developed technique based on image sequence analysis allows automatic and precise quantification of the dynamics growth velocity root tip, distribution expansion rates along entire zone oscillation frequencies tip during without need artificial landmarks. These three major parameters characterizing primary roots can be analysed over several days with high spatial (20 microm) temporal resolution (several minutes) as camera follows growing by an image-controlled tracking device. In...
The integrity of genome is a prerequisite for healthy life. Indeed, defects in DNA repair have been associated with several human diseases, including tissue-fibrosis, neurodegeneration and cancer. Despite decades extensive research, the spatio-mechanical processes double-strand break (DSB)-repair, especially auxiliary factor(s) that can stimulate accurate timely repair, remained elusive. Here, we report an ATM-kinase dependent, unforeseen function nuclear isoform Receptor Advanced Glycation...
We have compared the distribution of endogenous heterochromatin protein 1 (HP1) proteins (α, β and γ) in different epithelial lines, pluripotent stem cells embryonic fibroblasts. In parallel, we interrogated assembly dynamics newly expressed HP1-GFP lacking both HP1α HP1β alleles, blocked at G1-S boundary, or cultured presence HDAC HAT inhibitors. The results reveal a range cell type differentiation state-specific patterns that do not correlate with `fast' `slow' subunit exchange...
A modern day light microscope has evolved from a tool devoted to making primarily empirical observations what is now sophisticated , quantitative device that an integral part of both physical and life science research. Nowadays, microscopes are found in nearly every experimental laboratory. However, despite their prevalent use capturing quantifying scientific phenomena, neither thorough understanding the principles underlying imaging techniques nor appropriate knowledge how calibrate,...
SH4 domains provide bipartite membrane-targeting signals for oncogenic Src family kinases. Here we report the induction of non-apoptotic plasma membrane (PM) blebbing as a novel and conserved activity derived from prototypic kinases Src, Fyn, Yes Lck well HASPB protein Leishmania parasites. SH4-domain-induced is highly dynamic, with bleb formation collapse displaying distinct kinetics. These reorganizations PM are controlled by Rho but not Rac or Cdc42 GTPase signalling pathways. SH4-induced...
Modern life science research is a collaborative effort. Few groups can single-handedly support the necessary equipment, expertise and personnel needed for ever-expanding portfolio of technologies that are required across multiple disciplines in today's endeavours. Thus, institutes increasingly setting up scientific core facilities to provide access specialised cutting-edge technologies. Maintaining momentum carry out leading while ensuring high-quality daily operations an ongoing challenge,...
We have examined HP1β-chromatin interactions in different molecular contexts vitro and vivo. Employing purified components we show that HP1β exhibits selective, stoichiometric, salt-resistant binding to recombinant histone H3, associating primarily with the helical “histone fold” domain. Furthermore, using “bulk” nucleosomes released by MNase digestion, S-phase extracts, fragments of peripheral heterochromatin, demonstrate associates more tightly destabilized or disrupted (H3/H4...
Endothelial and epithelial barrier function is crucial for the maintenance of physiological processes. The paracellular permeability depends on composition spatial distribution cell-to-cell tight junctions (TJ). Here, we provide an experimental workflow that yields several layers data in setting a single endothelial cell monolayer. Human umbilical vein cells were grown Transwell filters. Transendothelial electrical resistance (TER) 10 kDa FITC dextran flux measured using Alanyl-Glutamine...
Operating shared resource laboratories (SRLs) in times of pandemic is a challenge for research institutions. In multiuser, high-turnover working space, the transmission infectious agents difficult to control. To address this challenge, imaging core facility managers being members German BioImaging discussed how microscopes could be operated with minimal risk spreading SARS-CoV-2 between users and staff. Here, we describe resulting guidelines explain their rationale, focus on separating space...
Acylated SH4 domains represent N-terminal targeting signals that anchor peripheral membrane proteins such as Src kinases in the inner leaflet of plasma membranes. Here we provide evidence for a novel regulatory mechanism may control levels being associated with Using fusion protein domain Leishmania HASPB and GFP model system, demonstrate threonine 6 is substrate phosphorylation. Substitution by glutamate (to mimic phosphothreonine residue) resulted dramatic redistribution from membranes to...
Fluorescence resonance energy transfer (FRET) efficiency measurements based on acceptor photobleaching of yellow fluorescent protein (YFP) are affected by the fact that bleaching YFP produces a species is detectable in cyan (CFP) image channels. The presented quantitative measurement this conversion makes it possible to correct obtained FRET signal increase accuracy intensity CFP/YFP measurements. described method can additionally be used compare samples with very different fluorescence levels.
INTRODUCTION Since the complete sequencing of several genomes, systematic understanding protein function and interaction has become one most important tasks in current molecular biology. Recent advances light microscopy equipment regarding automation acquisition speed offer possibility to perform hundreds experiments parallel study intact cells. In this context, live cell imaging is an excellent tool that may be used investigate dynamic or rarely occurring cellular processes, distinguish...
Growing tissues dominate sink activity during the vegetative phase of many plant species. Growth is characterised by its spatio-temporal dynamics. Thus, in order to understand sink-source interactions, growth processes have be quantified space and time. New techniques based on image sequence analysis been developed last years that allow for first time map expansion scales relevant mechanistic control biochemical molecular level. mapping done via motion optical flow-based structure tensor...