A5005577494- Cell Image Analysis Techniques
- Single-cell and spatial transcriptomics
- Cellular transport and secretion
- Alzheimer's disease research and treatments
- Cellular Mechanics and Interactions
- Image Processing Techniques and Applications
- Neurofibromatosis and Schwannoma Cases
- Global Health and Surgery
- Machine Learning in Bioinformatics
- Health and Medical Research Impacts
- Retinal Development and Disorders
- Genetics, Bioinformatics, and Biomedical Research
- Endometriosis Research and Treatment
- Bone Metabolism and Diseases
- Vitamin D Research Studies
- Uterine Myomas and Treatments
- Molecular Biology Techniques and Applications
- Endometrial and Cervical Cancer Treatments
- Neuroblastoma Research and Treatments
- Advanced Image Fusion Techniques
- Virus-based gene therapy research
- Image and Video Quality Assessment
- Polysaccharides and Plant Cell Walls
- Genetic diversity and population structure
- Mast cells and histamine
University of Helsinki
2022-2024
Aarhus University
2023-2024
University of Turku
2016-2023
Shahid Beheshti University of Medical Sciences
2019-2020
Åbo Akademi University
2015-2019
Turku Centre for Computer Science
2019
Ferdowsi University of Mashhad
2008
Measurement of changes pH at various intracellular compartments has potential to solve questions concerning the processing endocytosed material, regulation acidification process, and also vesicles destined for exocytosis. To monitor these events, nanosized optical probes need provide ratiometric signals in optically transparent biological window, target all relevant compartments, facilitate imaging subcellular resolution without interference from matrix. meet criteria we sensitize surface...
Abstract In the dynamic landscape of scientific research, imaging core facilities are vital hubs propelling collaboration and innovation at technology development dissemination frontier. Here, we present a collaborative effort led by Global BioImaging (GBI), introducing international recommendations geared towards elevating careers Imaging Scientists in facilities. Despite critical role modern research ecosystems, challenges persist recognising their value, aligning performance metrics...
SORL1 , the gene encoding large multidomain SORLA protein, has emerged as only fourth that when mutated can by itself cause Alzheimer’s disease (AD), and a reliably linked to both early- late-onset forms of disease. is known interact with endosomal trafficking regulatory complex called retromer in regulating recycling cargo, including amyloid precursor protein (APP) glutamate receptor GluA1. Nevertheless, SORLA’s precise structural–functional relationship tubules remains unknown. Here, we...
<ns3:p>The ability of cells to migrate is a fundamental physiological process involved in embryonic development, tissue homeostasis, immune surveillance, and wound healing. Therefore, the mechanisms governing cellular locomotion have been under intense scrutiny over last 50 years. One main tools this live-cell quantitative imaging, where researchers image time study their migration quantitatively analyze dynamics by tracking them using recorded images. Despite availability computational...
A modern day light microscope has evolved from a tool devoted to making primarily empirical observations what is now sophisticated , quantitative device that an integral part of both physical and life science research. Nowadays, microscopes are found in nearly every experimental laboratory. However, despite their prevalent use capturing quantifying scientific phenomena, neither thorough understanding the principles underlying imaging techniques nor appropriate knowledge how calibrate,...
Automated analysis of microscope images is necessitated by the increased need for high-resolution follow up events in time. Manually finding right to be analyzed, or eliminated from data are common day-to-day problems microscopy research today, and constantly growing size image datasets does not help matter. We propose a simple method software tool sorting within dataset, according their relative quality. demonstrate applicability our good quality STED sample preparation optimization...
SORLA, the protein encoded by
ABSTRACT The sphingolipids, sphingosine 1-phosphate (S1P) and sphingosylphosphorylcholine (SPC), can induce or inhibit cellular migration. intermediate filament protein vimentin is an inducer of migration a marker for epithelial–mesenchymal transition. Given that keratin filaments are regulated by SPC, with consequences cell motility, we wanted to determine whether also sphingolipid signalling it determinant sphingolipid-mediated functions. In cancer cells where S1P SPC inhibited migration,...
<ns3:p>The ability of cells to migrate is a fundamental physiological process involved in embryonic development, tissue homeostasis, immune surveillance, and wound healing. Therefore, the mechanisms governing cellular locomotion have been under intense scrutiny over last 50 years. One main tools this live-cell quantitative imaging, where researchers image time study their migration quantitatively analyze dynamics by tracking them using recorded images. Despite availability computational...
Bioimage analysis (BIA), a crucial discipline in biological research, overcomes the limitations of subjective microscopy through creation and application quantitative reproducible methods. The establishment dedicated BIA support within academic institutions is vital to improving research quality efficiency can significantly advance scientific discovery. However, lack training resources, limited career paths insufficient recognition contributions made by bioimage analysts prevent full...
Osteoclasts are multinucleated bone-resorbing cells with a dynamic actin cytoskeleton. derived from circulating mononuclear precursors. Confocal and stimulated emission depletion (STED) super-resolution microscopy was used to investigate peripheral blood-derived human osteoclasts cultured on glass surfaces. STED confocal demonstrated that the curved branched, for instance, in vicinity of membrane ruffles. The overall architecture network extended podosomes top cell. other novel finding...
Biocatalytic pulp fibers were prepared using surface functionalization of bleached kraft with amino groups (F) and further immobilization a cross-linked glucose oxidase (G*) from Aspergillus niger. The enzymes characterized X-ray spectroscopy, Fourier transform infrared dynamic scanning calorimetry, light scattering. According to standard assays, the G* content on resulting (FG*) was 11 mg/g fiber, enzyme activity 215 U/g. results confocal- stimulated emission depletion microscopy techniques...
Abstract To elucidate processes in the osteoclastic bone resorption, visualise resorption and related actin reorganisation, a combination of imaging technologies an applicable vitro model is needed. Nanosized powder from matching species deposited on any biocompatible surface order to form thin, translucent, smooth elastic representation injured bone. Osteoclasts cultured layer expressed morphology ones sawed cortical slices. Resorption pits were easily identified by reflectance microscopy....
Abstract Long noncoding RNAs (lncRNAs) are emerging as the master regulators of tumor initiation, proliferation, and metastasis; however, their diagnostic value potential biomarkers should be clarified. Vitamin D influences expression several genes in various pathways, including CYP24A1 gene vitamin metabolism pathway. In present research, we surveyed levels clinical significance novel lncRNAs related to PFDN4 colorectal cancer (CRC) using real‐time polymerase chain reaction. Furthermore,...
Cutaneous neurofibromas (cNFs) are hallmarks of neurofibromatosis 1 (NF1) and cause the main disease burden in adults with NF1. Mast cells a known component cNFs. However, no comprehensive characterization mast cNFs is available, their contributions to cNF growth symptoms such as itch not known.We collected 60 from ten individuals NF1, studied cell proteinase content, compared numbers selected clinical features tumors patients. The were immunolabeled for markers CD117, tryptase, chymase,...
Abstract Actin and myosins (IIA, IIB, X) generate mechanical forces in osteoclasts that drive functions such as migration membrane trafficking. In neurofibromatosis, these processes are perturbed due to a mutation neurofibromatosis type 1 (NF1) gene. This leads generation of hyperactive bone-resorbing increases incidence skeletal dysplasia e.g. early-onset osteoporosis patients suffering from neurofibromatosis. To study the density function actin clusters mutated cells we introduce new...
Cutaneous neurofibromas (cNFs) are characteristic of neurofibromatosis 1 (NF1), yet their immune microenvironment is incompletely known. A total 61 cNFs from 10 patients with NF1 were immunolabeled for different types T cells and macrophages, the cell densities correlated clinical characteristics. Eight overlying skin analyzed receptor CDR domain sequences, mass spectrometry 15 was performed to study immune-related processes. Intratumoral detected in all cNFs. Tumors individuals younger than...
In current research, genetic relationships among rapeseed genotypes from several geographical origins including France, Canada, Germany, Iran, Hungary, Denmark, Australia and America were evaluated using RAPD markers. Among generated 86 bands, 80 different polymorphic bands obtained 9 random primers. Diversity Index (DI) or Polymorphism Information Content (PIC) was varied 0.29 to 0.48, showed a relatively high potential of primers studied genotypes. Dice similarity coefficient between...
Aim: rs2585428 and rs4809960 polymorphisms were significantly associated with overall cancer risk, but there is no evidence regarding the colorectal (CRC) risk. Materials & methods: A total of 505 subjects, including 246 patients CRC 259 noncancer controls participated in study. The genotyping was performed using tetra-primer amplification refractory mutation systems PCR. Results: Analysis genotypes revealed that CYP24A1 CC genotype decreased risk (p = 0.009). In addition, frequencies showed...
The ability of cells to migrate is a fundamental physiological process involved in embryonic development, tissue homeostasis, immune surveillance, and wound healing. Therefore, the mechanisms governing cellular locomotion have been under intense scrutiny over last 50 years. One main tools this live-cell quantitative imaging, where researchers image time study their migration quantitatively analyze dynamics by tracking them using recorded images. Despite availability computational tools,...
Abstract Background As a novel class of non‐coding RNAs, the role circular RNAs (circRNAs) in tumor biogenesis and progression has been proved number human tumors; however, up to now, relation between circRNAs uterine leiomyomas (ULM) remains unclear. Methods In this study, we have estimated expression level CYP24A1 hsa_circ_0060927 leiomyoma adjacent tissues considering mediator complex subunit 12 gene (MED12) mutation profile by quantitative real‐time polymerase chain reaction (qRT‐PCRs)....