A5049271390- Osteoarthritis Treatment and Mechanisms
- Mesenchymal stem cell research
- Bone health and treatments
- Bone Metabolism and Diseases
- Lower Extremity Biomechanics and Pathologies
- Rheumatoid Arthritis Research and Therapies
- Bone and Joint Diseases
- Immune Response and Inflammation
- Cell Adhesion Molecules Research
- Musculoskeletal synovial abnormalities and treatments
- Force Microscopy Techniques and Applications
- Tissue Engineering and Regenerative Medicine
- Bone health and osteoporosis research
- Bone Tissue Engineering Materials
- Knee injuries and reconstruction techniques
- Orthopaedic implants and arthroplasty
- Orthopedic Infections and Treatments
- Radiomics and Machine Learning in Medical Imaging
- Medical Imaging and Analysis
- Neonatal Respiratory Health Research
- Total Knee Arthroplasty Outcomes
- Periodontal Regeneration and Treatments
- Vitamin D Research Studies
- Cancer, Hypoxia, and Metabolism
- Cancer Cells and Metastasis
Oulu University Hospital
2016-2025
University of Oulu
2016-2025
Institute of Clinical Research
2016
Institute for Biomedicine
2012
Nieuwegein Fonds
2007
Centre Hospitalier de Troyes
2007
University College London
1999-2003
University of Aberdeen
2002
University of Eastern Finland
2002
University of Turku
1998-2002
Knee osteoarthritis (OA) is the most common musculoskeletal disorder. OA diagnosis currently conducted by assessing symptoms and evaluating plain radiographs, but this process suffers from subjectivity. In study, we present a new transparent computer-aided method based on Deep Siamese Convolutional Neural Network to automatically score knee severity according Kellgren-Lawrence grading scale. We trained our using data solely Multicenter Osteoarthritis Study validated it randomly selected...
Osteoclasts are multinucleated cells responsible for bone resorption. During the resorption cycle, osteoclasts undergo dramatic changes in their polarity, and resorbing reveal four functionally structurally different membrane domains. Bone degradation products, both organic inorganic, were endocytosed from ruffled border membrane. They then found to be transported vesicles through cell plasma domain, located middle of basal membrane, where they liberated into extracellular space. These...
Inducement of foreign-body granulation tissue is a relatively novel therapeutic modality in bone repair. A two-stage reconstruction method, known as the Masquelet technique, combines inducement membrane and subsequent autografting biphasic technique allowing large defects. In light their already well-characterized osteogenesis-improving capabilities animals, we performed this translational study to investigate these membranes patients.Fourteen patients with complicated fractures defects were...
Bisphosphonates are currently the most important class of antiresorptive drugs used for treatment diseases with excess bone resorption. Recent studies have shown that bisphosphonates can be divided into two groups distinct molecular mechanisms action depending on nature R(2) side chain. Alendronate, like other nitrogen-containing bisphosphonates, inhibits resorption and causes apoptosis osteoclasts cells in vitro by preventing post-translational modification GTP-binding proteins isoprenoid...
Bisphosphonates are currently the most important class of antiresorptive drugs used for treatment diseases with excess bone resorption. On basis their molecular mechanism action, bisphosphonates can be divided into two pharmacological classes; nitrogen‐containing (N‐BPs) and non‐nitrogen‐containing (non‐N‐BP). Both classes induce apoptosis but they evoke it differently; N‐BPs by inhibiting intracellular mevalonate pathway protein isoprenylation, non‐N‐BPs via cytotoxic ATP analog‐type...
Tartrate-resistant acid phosphatase (TRAP) is highly expressed in bone-resorbing osteoclasts and activated macrophages. It has been suggested that a redox-active iron the binuclear center of TRAP could have capacity to react with hydrogen peroxide produce destructive reactive oxygen species (ROS). Here we show can generate ROS <i>in vitro</i> cells over-expressing higher amounts intracellular than their parent cells. We further demonstrate these be targeted destroy collagen other proteins....
Carbonic anhydrase III (CA III; EC 4.2.1.1) is a cytoplasmic enzyme that exhibits relatively low carbon dioxide hydratase activity. It expressed at very high level in skeletal muscle, where physical exercise has been shown to increase free radical production. In this work we show the effect of overexpression CA on cellular response oxidative stress. Rat cDNA was transfected NIH/3T3 cells, which have no endogenous expression. The isolated clones mRNA and protein. protein localized cytoplasm...
Abstract Human embryonic and mesenchymal stem cell therapies may offer significant benefit to a large number of patients. Recently, however, human lines cultured on mouse feeder cells were reported be contaminated by the xeno-carbohydrate N-glycolylneuraminic acid (Neu5Gc) considered potentially unfit for therapy. To determine extent problem Neu5Gc contamination development therapies, we investigated whether it also occurs in cells, what are sources contamination, is reversible. We found...
Abstract Human mesenchymal stem cells (hMSCs) are multipotent that have aroused great expectations in regenerative medicine. They assumed to originate from hypoxic cell niches, especially the bone marrow. This suggests O2 is of importance their regulation. In order characterize regulation oxygen sensing pathway these cells, we studied hMSCs isolated three origins, adult and pediatric marrow umbilical cord blood (UCB). Surprisingly, UCB MSCs showed normoxic stabilization hypoxia-inducible...
Abstract The promising clinical effects of mesenchymal stromal/stem cells (MSCs) rely especially on paracrine and nonimmunogenic mechanisms. Delivery routes are essential for the efficacy cell therapy systemic delivery by infusion is obvious goal many forms MSC therapy. Lung adhesion MSCs might, however, be a major obstacle yet to overcome. Current knowledge does not allow us make sound conclusions whether lung entrapment harmful or beneficial, thus we wanted explore in greater detail. We...
Resolution-phase macrophage population orchestrates active dampening of the inflammation by secreting anti-inflammatory and proresolving products including interleukin (IL)-10 lipid mediators (LMs). We investigated effects both human bone marrow-derived mesenchymal stromal cells (MSCs) MSC-derived extracellular vesicles (MSC-EVs) on mature regulatory macrophages (Mregs). The cytokines LMs were determined from cell culture media Mregs cultivated with MSCs MSC-EVs. In addition, alterations in...
Despite increasing evidence that subchondral bone contributes to osteoarthritis (OA) pathogenesis, little is known about local changes in structure compared cartilage degeneration. This study linked structural adaptation of with histological OA grade. Twenty-five osteochondral samples macroscopically different degeneration were prepared from tibiae 14 patients. Samples scanned micro-computed tomography (μCT) and both conventional parameters novel 3D based on patterns analyzed the plate...
Abstract Fiber-based scaffolds produced by textile manufacturing technology offer versatile materials for tissue engineering applications since a wide range of crucial scaffold parameters, including porosity, pore size and interconnectivity, can be accurately controlled using 3D weaving. In this study, we developed weavable, bioactive biodegradable composite fiber from poly (lactic acid) (PLA) hydroxyapatite powder melt spinning. Subsequently, these fibers were fabricated The differentiation...
Mesenchymal stem/stromal cells (MSCs) have the capacity to counteract excessive inflammatory responses. MSCs possess a range of immunomodulatory mechanisms, which can be deployed in response signals particular environment and concert with other immune cells. One immunosuppressive mechanism, not so well-known MSCs, is mediated via adenosinergic pathway by ectonucleotidases CD73 CD39. In this study, we demonstrate that adenosine actively produced from 5'-monophosphate (AMP) on MSC-derived...
Abstract Osteoarthritis (OA) is a common degenerative musculoskeletal disease highly prevalent in aging societies worldwide. Traditionally, knee OA diagnosed using conventional radiography. However, structural changes of articular cartilage or menisci cannot be directly evaluated this method. On the other hand, ultrasound promising tool able to provide direct information on soft tissue degeneration. The aim our study was systematically determine site-specific diagnostic performance...
Osteoarthritis (OA) has often regarded as a disease of articular cartilage only. New evidence shifted the paradigm towards system biology approach, where also surrounding tissue, especially bone is studied more vigorously. However, histological features subchondral are only poorly characterized in current grading scales OA. The aim this study to specifically characterize changes occurring at different stages OA and propose simple for them.20 patients undergoing total knee replacement surgery...
Systemic adenoviral delivery into tumors is inefficient because of liver sequestration intravenously administered virus. One potential solution for improving bioavailability the use carrier cells such as human mesenchymal stem (MSCs), which have been suggested to inherent tumor tropism. Here we investigated capacity capsid-modified adenoviruses infect and replicate in MSCs. Further, biodistribution tumor-killing efficacy MSCs loaded with oncolytic were evaluated orthotopic murine models lung...
Perfluoroalkyl substances (PFAS), including two most commonly studied compounds perfluorooctane sulfonate (PFOS) and perfluorooctanoic acid (PFOA), are widely distributed environmental pollutants, used extensively earlier. Due to their toxicological effects the use of PFAS is now regulated. Based on earlier studies PFOA's distribution in bone marrow mice, we investigated levels possible link microarchitecture human femoral samples (n = 18). Soft tissue biopsies were also taken from a 49-year...
Adipose tissue inflammation is an important pathological process in obese people, associated with diabetes and cardiovascular disease. We hypothesized that 1,25-dihydroxyvitamin D3 [1,25(OH)2D3] inhibits cytokine secretion from adipocytes via direct inhibition of transcription factor nuclear factor-κB (NF-κB). utilized two different human models. Bone marrow-derived mesenchymal stromal cells (hMSCs) differentiated into adipocytes, isolated biopsies stimulated lipopolysaccharide (LPS) were...