A5071059610- Photochromic and Fluorescence Chemistry
- Photoreceptor and optogenetics research
- Radical Photochemical Reactions
- Click Chemistry and Applications
- Chemical Synthesis and Analysis
- Asymmetric Synthesis and Catalysis
- Synthetic Organic Chemistry Methods
- Oxidative Organic Chemistry Reactions
- Advanced biosensing and bioanalysis techniques
- Luminescence and Fluorescent Materials
- bioluminescence and chemiluminescence research
- Advanced Synthetic Organic Chemistry
- Synthesis of Indole Derivatives
- Chemical synthesis and alkaloids
- Advanced Fluorescence Microscopy Techniques
- Chemical Synthesis and Reactions
- DNA and Nucleic Acid Chemistry
- Carbohydrate Chemistry and Synthesis
- Nanoplatforms for cancer theranostics
- Molecular spectroscopy and chirality
- Traditional and Medicinal Uses of Annonaceae
- Alkaloids: synthesis and pharmacology
- Biopolymer Synthesis and Applications
- Synthesis and Reactions of Organic Compounds
- Bioactive Compounds and Antitumor Agents
Toho University
2013-2024
Toyota Memorial Hospital
2022
Kariya Toyota General Hospital
2003-2011
Nagoya University
2011
Tokyo Medical and Dental University
2011
Osaka University
2010
Nagoya City University
2009
National Institute of Health Sciences
2009
Japan Science and Technology Agency
2001-2004
Howard Hughes Medical Institute
1999
Photochemical release (uncaging) of bioactive messengers with three-dimensional spatial resolution in light-scattering media would be greatly facilitated if the photolysis could powered by pairs IR photons rather than customary single UV photons. The quadratic dependence on light intensity confine to focus point laser, and longer wavelengths much less affected scattering. However, previous caged have had very small cross sections for two-photon excitation region. We now show that brominated...
Caged compounds can be used to regulate the spatial and temporal dynamics of signaling molecules in live cells. Photochemical properties coumarin-4-ylmethoxy carbonates (1a-d) are investigated construct caged hydroxy-containing molecules. All possess desired as phototriggers for alcohols phenols. The 6-bromo-7-hydroxycoumarin-4-ylmethoxycarbonyl (Bhcmoc) group has highest photochemical efficiency is applied make 1,2-dioctanoylglycerol (diC(8)), Tyr-OMe, adenosine. [reaction: see text]
Eggs of many marine and mammalian species attract sperm by releasing chemoattractants that modify the bending properties flagella to redirect paths toward egg. This process, called chemotaxis, is dependent on extracellular Ca2+. We used stroboscopic fluorescence imaging measure intracellular Ca2+ concentration ([Ca2+]i) in swimming sea urchin sperm. Uncaging cyclic GMP induced entry via at least two distinct pathways, we identified a nimodipine-sensitive pathway, compartmentalized flagella,...
Here, we demonstrated photoinduced NO generation from a 2,6-dimethylnitrobenzene-based compound (Flu-DNB) via two-photon excitation (TPE) process. After pulse laser irradiation to solution of Flu-DNB, oxidation products were observed. This is the first account non-nitrosyl-chelated metal ion containing donor which can be controlled by TPE technique.
cAMP is one of the most important second messengers in biological processes. Cellular dynamics have been investigated using a series fluorescent indicators; however, their sensitivity was sub-optimal for detecting at low concentration range, due to ligand affinity and/or poor dynamic range. Seeking an indicator with improved detection sensitivity, we performed insertion screening circularly permuted mApple, red protein, into cAMP-binding motif PKA regulatory subunit Iα and developed named...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTPhotochemical Properties of New Photolabile cAMP Derivatives in a Physiological Saline SolutionToshiaki Furuta, Hiromi Torigai, Masazumi Sugimoto, and Michiko IwamuraCite this: J. Org. Chem. 1995, 60, 13, 3953–3956Publication Date (Print):June 1, 1995Publication History Published online1 May 2002Published inissue 1 June 1995https://pubs.acs.org/doi/10.1021/jo00118a008https://doi.org/10.1021/jo00118a008research-articleACS PublicationsRequest reuse...
Abstract Cyclic nucleoside monophosphates (cNMPs) play key roles in many cellular regulatory processes, such as growth, differentiation, motility, and gene expression. Caged derivatives that can be activated by irradiation could powerful tools for studying diverse functions of intracellular second messengers, since the spatiotemporal dynamics these molecules controlled with appropriately focused light. Here we report synthesis, photochemistry, biological testing...
KikGR is a fluorescent protein engineered to display green-to-red photoconvertibility that induced by irradiation with ultraviolet or violet light. Similar Kaede and EosFP, two naturally occurring photoconvertible proteins, contains His62-Tyr63-Gly64 tripeptide sequence, which forms green chromophore can be photoconverted red one via formal β-elimination subsequent extension of π-conjugated system. Using crystallizable variant KikGR, we determined the structures both state at 1.55 Å...
A novel photocontrolled compound release system using liposomes and a caged antimicrobial peptide was developed. The activated by UV irradiation, resulting in the formation of pores on liposome surface to contained fluorophores. could be observed fluorescence measurements time-lapse microscopy. irradiation resulted quick inclusion compounds (within 1 min most cases) under simulated physiological conditions. proposed is expected applicable wide range fields from cell biology clinical sciences.
[see structure]. Synthesis and photochemistry of a new photochemically removable protecting group for alcohols is described. Four carbonates galactose derivatives (1-4) were synthesized from the corresponding arylmethanols via 4-nitrophenyl carbonate intermediates. Among them, photolysis anthraquinon-2-ylmethoxycarbonyl (Aqmoc) (1) proceeded with overall efficiency 150 (quantum yield 0.10, molar absorptivity 1500 M(-1) x cm(-1)) rate constant approximately 10(6) s(-1). To demonstrate its...
We report here the syntheses and photolytic properties of 3-(4,5-dimethoxy-2-nitrophenyl)-2-butyl (DMNPB) esters as new photoremovable groups for carboxylic acids, their use caging L-glutamate. A high-yielding synthesis DMNPB led to a 4:1 threo/erythro diastereomeric mixture, which could be separated by HPLC. While these were stable in neutral buffer, photolysis at 364 nm induced > or =95 % release acid, with 0.26 quantum yield L-glutamate formation. L-Glutamate was also possible two-photon...
Synthesis and photochemical properties of new photoremovable protecting groups for nucleobases are described. Four caged 2'-deoxycytidines (dCs) were synthesized, their measured under simulated physiological conditions. Two coumarin-caged dCs show better photophysical than those the having previously reported caging groups.
A new method for site-specific caging of plasmid DNA was developed to enable light-activation gene expression in living cells by exposure a low dose light.
Described is the development of 8-azacoumarin-4-ylmethyl groups as aqueous photolabile protecting groups. A key feature strategy isosteric replacement C7–C8 enol double bond Bhc derivative with an amide bond, resulting in conversion chromophore from coumarin to 8-azacoumarin. This makes dramatically enhanced water solubility and facile photocleavage possible.
Rapid and efficient light-induced fluorescence enhancement is demonstrated on a DMNPB-"caged" coumarin derivative carrying His-tag recognition motif.