A5038140792- Cannabis and Cannabinoid Research
- Prenatal Substance Exposure Effects
- Plant Surface Properties and Treatments
- Virus-based gene therapy research
- Neuroscience of respiration and sleep
- Bacteriophages and microbial interactions
- Postharvest Quality and Shelf Life Management
- Forensic Toxicology and Drug Analysis
- Muscle Physiology and Disorders
- Lipid metabolism and biosynthesis
- DNA Repair Mechanisms
- Viral Infections and Outbreaks Research
- Sperm and Testicular Function
- Inflammatory Myopathies and Dermatomyositis
- Pancreatic function and diabetes
- Plant biochemistry and biosynthesis
- Pain Mechanisms and Treatments
- Plant Virus Research Studies
- Insect-Plant Interactions and Control
- Alcohol Consumption and Health Effects
- Plant Molecular Biology Research
- Ion Channels and Receptors
- CRISPR and Genetic Engineering
- Cardiomyopathy and Myosin Studies
- Reproductive System and Pregnancy
National Taiwan University of Science and Technology
2025
Nationwide Children's Hospital
2022-2024
University of British Columbia
2006-2022
Case Western Reserve University
2016-2019
University of Leicester
2005-2016
George Washington University
2016
Case Comprehensive Cancer Center
2016
Leicester Royal Infirmary
2005-2011
University Hospitals of Leicester NHS Trust
2011
Plant Biotechnology Institute
2009
Abstract A waxy cuticle that serves as a protective barrier against uncontrolled water loss and environmental damage coats the aerial surfaces of land plants. It is composed cutin polymer matrix waxes. Cuticular waxes are complex mixtures very-long-chain fatty acids their derivatives. We report here molecular cloning characterization CER4, wax biosynthetic gene from Arabidopsis (Arabidopsis thaliana). cer4 mutants exhibit major decreases in stem primary alcohols esters, slightly elevated...
Abstract Wax esters are neutral lipids composed of aliphatic alcohols and acids, with both moieties usually long-chain (C16 C18) or very-long-chain (C20 longer) carbon structures. They have diverse biological functions in bacteria, insects, mammals, terrestrial plants also important substrates for a variety industrial applications. In plants, wax mostly found the cuticles coating primary shoot surfaces, but they accumulate to high concentrations seed oils few plant species, including jojoba...
Aims: Urinary 8-oxo-7,8-dihydro-2′-deoxyguanosine (8-oxodG) is a widely used biomarker of oxidative stress. However, variability between chromatographic and ELISA methods hampers interpretation data, this may increase should urine composition differ individuals, leading to assay interference. Furthermore, optimal sampling conditions are not well defined. We performed inter-laboratory comparisons 8-oxodG measurement mass spectrometric-, electrochemical- ELISA-based methods, using common...
Abstract The cuticle is an extracellular matrix composed of cutin polyester and waxes that covers aerial organs land plants protects them from environmental stresses. Arabidopsis thaliana cer7 mutant exhibits reduced cuticular wax accumulation contains considerably lower transcript levels ECERIFERUM3/WAX2/YORE-YORE (CER3/WAX2/YRE), a key biosynthetic gene. We show here CER7 protein putative 3′-5′ exoribonuclease homologous to yeast Ribonuclease PH45 (RRP45p), core subunit the RNA processing...
Abstract The cuticle is a protective layer that coats the primary aerial surfaces of land plants and mediates plant interactions with environment. It synthesized by epidermal cells composed cutin polyester matrix embedded covered cuticular waxes. Recently, we have discovered novel regulatory mechanism wax biosynthesis involves ECERIFERUM7 (CER7) ribonuclease, core subunit exosome. We hypothesized at onset production, CER7 ribonuclease degrades an mRNA specifying repressor CER3, biosynthetic...
While highly promising in medicine, gene therapy requires delivery agents to protect and target nucleic acid therapeutics.
The concurrent impact of repeated low-level summer sunlight exposures on vitamin D production and cutaneous DNA damage, potentially leading to mutagenesis skin cancer, is unknown.This an experimental study (i) determine the dual status damage/repair (via both urinary biomarkers) in light-skinned adults; (ii) compare outcomes following same brown-skinned adults.Ten white (phototype II) six South Asian volunteers V), aged 23-59 years, received 6 weeks' simulated (95% ultraviolet A/5% B, 1·3...
When results of laboratory experiments on the effects nutrient enrichment algal cultures are extrapolated to field conditions, predictions seldom fulfilled, because behavior a species in network interacting variables nature is quite different from its under controlled conditions. The method loop analysis used demonstrate properties set models containing three kinds algae and nutrients. Many unexpected develop interactions within network, one‐link physiological cannot be ecological...
CELLULOSE SYNTHASE5 (CESA5) synthesizes cellulose necessary for seed mucilage adherence to coat epidermal cells of Arabidopsis (Arabidopsis thaliana). The involvement additional CESA proteins in this process and details concerning the manner which is deposited pocket are unknown. Here, we show that both CESA3 CESA10 highly expressed cell type at time synthesis localize plasma membrane adjacent pocket. isoxaben resistant1-1 resistant1-2 mutants affecting defects consistent with altered...
The primary aerial surfaces of land plants are covered with a cuticle, protective layer composed the cutin polyester matrix and cuticular waxes. Previously, we discovered unique mechanism regulating wax biosynthesis during Arabidopsis (Arabidopsis thaliana) stem elongation that involves ECERIFERUM7 (CER7), core subunit exosome. Because loss-of-function mutations in CER7 result reduced expression biosynthetic gene CER3, proposed is involved degrading messenger RNA encoding CER3 repressor. To...
Photodynamic therapy (PDT) is a promising avenue for greater treatment efficacy of highly resistant and aggressive melanoma. Through photosensitizer attachment to nanoparticles, specificity delivery can be conferred further reduce potential side effects. While the main focus PDT destruction cancer cells, additional targeting tumor-associated macrophages also present in tumor microenvironment could enhance by eliminating their role processes such as invasion, metastasis, immunosuppression. In...
Mitoxatrone (MTO), an antineoplastic chemotherapeutic, has potent activity against the most common and agressive type of primary brain tumor, glioblastoma multiforme (GBM). However, its poor penetration through blood barrier, cardiotoxic side effects from systemic delivery limit effectiveness for clinical treatment. To address these limitations, we utilize a plant virus-based nanoparticle, cowpea mosaic virus (CPMV), to deliver MTO treat GBM. In this work, loaded into interior cavity CPMV...
Interaction of reactive oxygen species with DNA results in a variety modifications, including 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG), which has been extensively studied as biomarker oxidative stress. Oxidative stress is implicated number pathophysiological processes relevant to obstetrics and gynecology; however, there lack understanding the precise role these processes. We aimed develop rapid, validated assay for accurate quantification 8-oxodG human urine using solid-phase...
In early pregnancy, increased plasma levels of the endocannabinoid anandamide (AEA) are associated with miscarriage through mechanisms that might affect developing placenta or maternal decidua. this study, we compare AEA in failed and viable pregnancies trophoblastic hormones (beta-human chorionic gonadotrophin (beta-hCG), progesterone (P4) (pregnancy-associated placental protein-A (PAPP-A)) essential for pregnancy success relate to expression cannabinoid receptors enzymes modulate levels....
Abstract As metagenomic approaches for detecting infectious agents have improved, each tissue that was once thought to be sterile has been found harbor a variety of microorganisms. Controversy still exists over the status amniotic fluid, which is part an immunologically privileged zone required prevent maternal immune system rejection fetus. Due this privilege, exclusion microbes proposed mandatory, leading womb hypothesis. Since nucleic acid yields from fluid are very low, contaminating in...