A5102938430- DNA Repair Mechanisms
- Carcinogens and Genotoxicity Assessment
- Skin Protection and Aging
- DNA and Nucleic Acid Chemistry
- Vitamin C and Antioxidants Research
- Cytomegalovirus and herpesvirus research
- Folate and B Vitamins Research
- Effects and risks of endocrine disrupting chemicals
- Health, Environment, Cognitive Aging
- Genomics, phytochemicals, and oxidative stress
- Antioxidant Activity and Oxidative Stress
- Metabolomics and Mass Spectrometry Studies
- Molecular Biology Techniques and Applications
- bioluminescence and chemiluminescence research
- Epigenetics and DNA Methylation
- Photodynamic Therapy Research Studies
- CRISPR and Genetic Engineering
- Cancer, Hypoxia, and Metabolism
- Advanced biosensing and bioanalysis techniques
- Mass Spectrometry Techniques and Applications
- Acute Lymphoblastic Leukemia research
- Biochemical and Molecular Research
- Glutathione Transferases and Polymorphisms
- Smoking Behavior and Cessation
- RNA modifications and cancer
University of South Florida
2020-2025
Moffitt Cancer Center
2024-2025
University of Leicester
2009-2024
Florida International University
2015-2024
University Hospitals of Leicester NHS Trust
2002-2024
Leicester Royal Infirmary
2000-2024
Eastern Virginia Medical School
2012
University of Leeds
2009-2010
Collegium Medicum in Bydgoszcz
2010
Centre International de Recherche sur le Cancer
2010
Selenium, a trace element that is fundamental to human health, incorporated into some proteins as selenocysteine (Sec), generating family of selenoproteins. Sec incorporation mediated by multiprotein complex includes insertion sequence-binding protein 2 (SECISBP2; also known SBP2). Here, we describe subjects with compound heterozygous defects in the SECISBP2 gene. These individuals have reduced synthesis most 25 selenoproteins, resulting phenotype. Azoospermia, failure latter stages...
There appears to be a paucity of data examining the effect dietary antioxidants on levels oxidative DNA damage in vivo, limiting evidence‐based assessment antioxidant efficacy, mechanisms and recommendation for optimal intake. We have examined 8‐oxo‐2′‐deoxyguanosine (8‐oxodG) mononuclear cell DNA, serum urine from subjects undergoing supplementation with 500 mg/day vitamin C. Significant decreases 8‐oxodG were seen, correlating strongly increases plasma C concentration. Furthermore we...
The European Standards Committee on Oxidative DNA Damage (ESCODD) was set up to resolve the problems associated with measurement of background levels oxidative damage (in particular 8-oxo-7,8-dihydroguanine, or 8-oxoGua) in human cells. A tendency for oxidation occur during sample preparation prior chromatography has been recognized as source a very substantial artefact. To assess success attempts eliminate artefact, ESCODD distributed its members standard samples pig liver and HeLa cells...
SummaryThe bacterium Serratia marcescens has been used as a test system for demonstrating the radiosensitizing properties of class electron-affinic organic compounds. These include: unsaturated diesters and diketo compounds, mono-, di- triketohydrindene (indanetrione), derivatives pyruvic acid, quinones several miscellaneous With few exceptions, sensitization is restricted to anoxic systems. An electron-transfer mechanism, involving interaction sensitizer with charged intermediate produced...
Aims: Urinary 8-oxo-7,8-dihydro-2′-deoxyguanosine (8-oxodG) is a widely used biomarker of oxidative stress. However, variability between chromatographic and ELISA methods hampers interpretation data, this may increase should urine composition differ individuals, leading to assay interference. Furthermore, optimal sampling conditions are not well defined. We performed inter-laboratory comparisons 8-oxodG measurement mass spectrometric-, electrochemical- ELISA-based methods, using common...
Of the DNA-derived biomarkers of oxidative stress, urinary 8-oxo-7,8-dihydro-2′-deoxyguanosine (8-oxodG) is most frequently measured. However, there significant discrepancy between chromatographic and immunoassay approaches, intratechnique agreement among all available chromatography-based assays ELISAs yet to be established. This a obstacle their use in large molecular epidemiological studies. To evaluate accuracy intra/intertechnique interlaboratory measurements, samples phosphate buffered...
Oxidatively generated damage to DNA has been implicated in the pathogenesis of a wide variety diseases. Increasingly, interest is also focusing upon effects other nucleic acids, RNA and (2'-deoxy-)ribonucleotide pools, evidence growing that these too may have an important role disease. LC-MS/MS ability provide absolute quantification specific biomarkers, such as 8-oxo-7,8-dihydro-2'-deoxyGuo (8-oxodG), both nuclear mitochondrial DNA, 8-oxoGuo RNA. However, significant quantities tissue are...
Endogenous electrophiles, ionizing and non-ionizing radiation, hazardous chemicals present in the environment diet can damage DNA by forming covalent adducts. adducts form critical cancer driver genes and, if not repaired, may induce mutations during cell division, potentially leading to onset of cancer. The detection quantification specific are some first steps studying their role carcinogenesis, physiological conditions that lead production, risk assessment exposure genotoxic chemicals....
Although exposure to exocannabinoids (e.g. marijuana) is associated with adverse pregnancy outcome, little known about the biochemistry, physiology, and consequences of endocannabinoids in human pregnancy. In these studies, we measured levels endocannabinoid anandamide (N-arachidonoylethanolamine, AEA) by HPLC-mass spectrometry 77 pregnant 25 nonpregnant women. The mean +/- sem plasma AEA first, second, third trimesters were 0.89 0.14, 0.44 0.12, 0.42 0.11 nm, respectively. first trimester...
Rationale: Asthma is characterized by disordered airway physiology as a consequence of increased smooth muscle contractility. The underlying cause this hypercontractility poorly understood.Objectives: We sought to investigate whether the burden oxidative stress in asthma heightened and mediated an intrinsic abnormality promoting hypercontractility.Methods: examined bronchial biopsies primary cells from subjects with healthy controls. determined expression targets implicated control their...
Monitoring oxidative stress in vivo is made easier by the ability to use samples obtained non-invasively, such as urine. The analysis of DNA oxidation, measurement oxidized 2′-deoxyribonucleosides urine, particularly 8-oxo-7,8-dihydro-2′-deoxyguanosine (8-oxodG), has been reported extensively literature many situations relating various pathologies, populations and environmental exposures. Understanding origins urinary 8-oxodG, other than it simply being a marker oxidation or its synthetic...
There are substantial inter-laboratory variations in the levels of DNA damage measured by comet assay. The aim this study was to investigate whether adherence a standard assay protocol would reduce variation reported values damage. Fourteen laboratories determined baseline level strand breaks (SBs)/alkaline labile sites and formamidopyrimidine glycosylase (FPG)-sensitive coded samples mononuclear blood cells (MNBCs) from healthy volunteers. were technical problems seven adopting protocol,...