A5010803046- Carcinogens and Genotoxicity Assessment
- DNA Repair Mechanisms
- Neuroscience and Neuropharmacology Research
- Cholinesterase and Neurodegenerative Diseases
- Alzheimer's disease research and treatments
- Antioxidant Activity and Oxidative Stress
- Phytochemicals and Antioxidant Activities
- Edible Oils Quality and Analysis
- Effects and risks of endocrine disrupting chemicals
- Memory and Neural Mechanisms
- Skin Protection and Aging
- Telomeres, Telomerase, and Senescence
- Receptor Mechanisms and Signaling
- Biochemical effects in animals
- Collagen: Extraction and Characterization
- Genomics, phytochemicals, and oxidative stress
- Molecular Biology Techniques and Applications
- Advanced Glycation End Products research
- Metabolomics and Mass Spectrometry Studies
- Mitochondrial Function and Pathology
- PARP inhibition in cancer therapy
- Neuroinflammation and Neurodegeneration Mechanisms
- MicroRNA in disease regulation
- DNA and Nucleic Acid Chemistry
- Tea Polyphenols and Effects
University of Florence
2015-2024
Italian Society of Physiotherapy
2021
Cambridge University Press
2009-2010
New York University Press
2009-2010
Agenzia Regionale per la Protezione Ambientale
2001-2007
Agenzia Regionale per la Protezione Ambientale della Toscana
2007
Angiologica (Italy)
2004
Stefan University
2003
Sapienza University of Rome
2003
University of Surrey
2001
ou non, émanant des établissements d'enseignement et de recherche français étrangers, laboratoires publics privés.
The European Standards Committee on Oxidative DNA Damage (ESCODD) was set up to resolve the problems associated with measurement of background levels oxidative damage (in particular 8-oxo-7,8-dihydroguanine, or 8-oxoGua) in human cells. A tendency for oxidation occur during sample preparation prior chromatography has been recognized as source a very substantial artefact. To assess success attempts eliminate artefact, ESCODD distributed its members standard samples pig liver and HeLa cells...
Extra-virgin olive oils (EVOO), high in phenolic compounds with antioxidant properties, could be partly responsible for the lower mortality and incidence of cancer CVD Mediterranean region. The present study aims to measure oxidative DNA damage healthy human subjects consuming different concentrations natural phenols. A randomised cross-over trial high-phenol EVOO (high-EVOO; 592 mg total phenols/kg) v. low-phenol (low-EVOO; 147 mg/kg) was conducted ten postmenopausal women Florence....
During the last several years, evidence that various enzymes hydrolyze NAD into bioactive products prompted scientists to revisit or design strategies able increase intracellular availability of dinucleotide. However, plasma membrane permeability and mitochondrial origin dinucleotide still wait be clearly defined. Here, we report contents increased upon exposure cell lines primary cultures exogenous (eNAD). precursors could not reproduce effects eNAD, they were found in incubating medium...
The comet assay or single cell gel electrophoresis, is the most common method used to measure strand breaks and a variety of other DNA lesions in human populations. To estimate risk overall mortality, mortality by cause, cancer incidence associated damage, cohort 2,403 healthy individuals (25,978 person-years) screened 16 laboratories using between 1996 2016 was followed-up. Kaplan-Meier analysis indicated worse survival medium high tertile damage (p < 0.001). effect on modelled according...
Oxidative stress, defined as an imbalance between the production of reactive oxygen species (ROS) and antioxidant defense mechanisms, plays a major role in inducing oxidative damage cellular impairment, resulting general decline physiological functions. The aim this work was to evaluate age-related changes circulating ROS levels plasma protein carbonyls, very young (2 months aged), (8 aged) middle age (15 F344 rats. In addition, DNA marker 8-hydroxy-2'-deoxyguanosine (8-OHdG) expression...
Biomonitoring of human populations exposed to chemical substances that can act as potential mutagens or carcinogens, may enable the detection damage and early disease prevention. In recent years, comet assay has become an important tool for assessing DNA damage, both in environmental occupational exposure contexts. To evidence role biomonitoring, we have analysed original research studies used their assessments, following PRISMA-ScR method (preferred reporting items systematic reviews...
In order to evaluate dietary and lifestyle determinants of oxidative DNA damage we used a modification the 'comet assay' (single cell alkaline gel electrophoresis), with fpg enzyme (formamidopyrimidine glycosilase), measure basal level oxidation in peripheral lymphocytes donated by 71 healthy adults living Florence, Italy. Detailed information about habits was collected two validated standardized questionnaires; also measured plasma concentrations selected micro-nutrients (six carotenoids,...
Recent research has focused on natural compounds possibly endowed with antiaging effects. Resveratrol is a stilbene compound produced by different plants many biologic activities, including an effect, which been demonstrated both in vitro eukaryotic cells and vivo mice. We studied the effect of resveratrol cultured human MRC5 fibroblasts, widely used model aging studies. The chronic treatment until senescence 5 μM induced small increase total number replications completed cultures at...
The aim of this study was to evaluate the effects olive oil phenols on brain aging in mice and verify whether antioxidant antiinflammatory activities these polyphenols were involved. C57Bl/6J fed from middle age senescence with extra-virgin (10% wt/wt dry diet) rich (total polyphenol dose/day, 6 mg/kg). Behavioral tests employed assess cognitive, motor, emotional behavior after or 12 months treatment. Parameters oxidative status inflammation measured different areas at same times evaluated...