A5045811686- Polysaccharides and Plant Cell Walls
- Plant Molecular Biology Research
- Plant nutrient uptake and metabolism
- Plant Reproductive Biology
- Photosynthetic Processes and Mechanisms
- Plant Gene Expression Analysis
- Plant tissue culture and regeneration
- Legume Nitrogen Fixing Symbiosis
- Lignin and Wood Chemistry
- Plant-Microbe Interactions and Immunity
VIB-UGent Center for Plant Systems Biology
2024
Ghent University
2024
University of British Columbia
2014-2021
University of Amsterdam
2021
Interactions between cell wall polymers are critical for establishing integrity and cell-cell adhesion. Here, we exploit the Arabidopsis (Arabidopsis thaliana) seed coat mucilage system to examine polymer interactions. On hydration, seeds release an adherent layer strongly attached in addition a nonadherent that can be removed by gentle agitation. Rhamnogalacturonan I (RG I) is primary component of mucilage, with homogalacturonan, cellulose, xyloglucan constituting minor components. Adherent...
CELLULOSE SYNTHASE5 (CESA5) synthesizes cellulose necessary for seed mucilage adherence to coat epidermal cells of Arabidopsis (Arabidopsis thaliana). The involvement additional CESA proteins in this process and details concerning the manner which is deposited pocket are unknown. Here, we show that both CESA3 CESA10 highly expressed cell type at time synthesis localize plasma membrane adjacent pocket. isoxaben resistant1-1 resistant1-2 mutants affecting defects consistent with altered...
Cell-to-cell adhesion is essential for establishment of multicellularity. In plants, such mediated through a middle lamella composed primarily pectic polysaccharides. The molecular interactions that influence cell-to-cell are not fully understood. We have used Arabidopsis (Arabidopsis thaliana) seed coat mucilage as model system to investigate between cell wall carbohydrates. Using forward-genetic approach, we discovered gene, RUBY PARTICLES IN MUCILAGE (RUBY), encoding protein annotated...
Galactose oxidases (GalOxs) are well-known enzymes that have been identified in several fungal species and characterized using structural enzymatic approaches. However, until very recently, almost no information on their biological functions was available. The Arabidopsis (Arabidopsis thaliana) gene ruby particles mucilage (RUBY) encodes a putative plant GalOx is required for pectin cross-linking through modification of galactose (Gal) side chains promotes cell-cell adhesion between seed...
Arabidopsis seed coat epidermal cells deposit a significant quantity of mucilage, composed the cell wall components pectin, hemicellulose, and cellulose, into apoplast during development. When mature seeds are hydrated, mucilage extrudes to form gelatinous capsule around seed. Determining monosaccharide composition both extruded whole is an essential technique for characterizing developmental processes mutants with altered composition. This protocol covers growth plants produce suitable...