A5077910598- Carcinogens and Genotoxicity Assessment
- Air Quality and Health Impacts
- DNA Repair Mechanisms
- Epigenetics and DNA Methylation
- Toxic Organic Pollutants Impact
- Metabolomics and Mass Spectrometry Studies
- Oral Health Pathology and Treatment
- Health, Environment, Cognitive Aging
- Folate and B Vitamins Research
- Mass Spectrometry Techniques and Applications
- Genomics, phytochemicals, and oxidative stress
- Vehicle emissions and performance
- Smoking Behavior and Cessation
- Plant-based Medicinal Research
- Atmospheric chemistry and aerosols
- Environmental Toxicology and Ecotoxicology
- Sperm and Testicular Function
- Amino Acid Enzymes and Metabolism
- Pesticide Exposure and Toxicity
- RNA modifications and cancer
- Cytomegalovirus and herpesvirus research
- Carbon and Quantum Dots Applications
- Analytical chemistry methods development
- Glutathione Transferases and Polymorphisms
- Advanced Chemical Sensor Technologies
Chung Shan Medical University
2015-2025
Chung Shan Medical University Hospital
2013-2025
Institute of Tibetan Plateau Research
2019
Chinese Academy of Sciences
2019
Beijing Research Center for Agricultural and Standards and Testing
2019
Florida International University
2018
Institute of Labor, Occupational Safety And Health, Ministry of Labor
2015
Old Dominion University
2015
National Health Research Institutes
2003-2004
National Cheng Kung University
2000-2002
Aims: Urinary 8-oxo-7,8-dihydro-2′-deoxyguanosine (8-oxodG) is a widely used biomarker of oxidative stress. However, variability between chromatographic and ELISA methods hampers interpretation data, this may increase should urine composition differ individuals, leading to assay interference. Furthermore, optimal sampling conditions are not well defined. We performed inter-laboratory comparisons 8-oxodG measurement mass spectrometric-, electrochemical- ELISA-based methods, using common...
Oxidatively generated damage to DNA has been implicated in the pathogenesis of a wide variety diseases. Increasingly, interest is also focusing upon effects other nucleic acids, RNA and (2'-deoxy-)ribonucleotide pools, evidence growing that these too may have an important role disease. LC-MS/MS ability provide absolute quantification specific biomarkers, such as 8-oxo-7,8-dihydro-2'-deoxyGuo (8-oxodG), both nuclear mitochondrial DNA, 8-oxoGuo RNA. However, significant quantities tissue are...
IARC has classified the betel nut as a human environmental carcinogen. Previous studies have found that arecoline (AR) is major alkaloid present in saliva of quid chewers. Saliva contains large content AR which been further shown to cause mutation oral mucosa cells, resulting cancer. Whereas, date, there are only few reported hepatotoxicity associated with and chewing. Therefore, main purpose this study was determine toxic effects its oxidative metabolite, N-oxide (ARNO), normal liver cell...
Abstract A highly sensitive and selective method, using isotope‐dilution liquid chromatography with tandem mass spectrometry (LC/MS/MS), for quantification of urinary 8‐hydroxy‐2′‐deoxyguanosine (8‐OHdG), an important biomarker oxidative stress, was developed compared a method enzyme‐linked immunosorbent assay (ELISA). The synthesis 15 N 5 ‐8‐OHdG is described. In this study, 140 urine samples were collected from workers in coke oven plant, including 49 control 91 who had been occupationally...
A reliable and fast liquid chromatography-tandem mass spectrometry method has been developed for the simultaneous determination of three oxidized nucleic acid damage products in urine, 8-oxoguanine (8-oxoGua), 8-oxo-7,8-dihydro-2′-deoxyguanosine (8-oxodGuo) 8-oxo-7,8-dihydroguanosine (8-oxoGuo). We applied this to assess effect various urine workup procedures on urinary concentrations products. Our results showed that frozen samples must be warmed (i.e., 37 °C) re-dissolve any precipitates...
Occupational exposure to nickel compounds has been associated with lung cancer. The correlation between high levels and increased risk of cancer previously reported in a case-control study. This study assessed whether the occurrence p53 mutations due DNA repair inhibition by nickel. A total 189 patients were enrolled determine tumor-adjacent normal tissues mutation status tumors through atomic absorption spectrometry direct sequencing, respectively. Nickel mutant significantly higher than...
The exposome represents the totality of endogenous and exogenous exposures across lifespan. These may result in DNA RNA damage, form adducts, which is a key factor etiology variety human diseases, including cancer. It understood that, following their repair, nucleic acid adducts are excreted into urine, making urine an ideal, non-invasive matrix to study whole-body adductome (the adducts). However, measurement these presents challenges due interference chemical nature spectrum...
Quantification of 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodGuo) in urine or blood is used to assess and monitor oxidative stress patients. We describe the use on-line solid-phase extraction (SPE) isotope-dilution liquid chromatography-tandem mass spectrometry (LC-MS/MS) for automated measurement urinary 8-oxodGuo.Automated purification was accomplished with a switching valve an Inertsil ODS-3 column. After addition 15N5-labeled 8-oxodGuo as internal standard, samples were analyzed within...