A5010507516- Estrogen and related hormone effects
- Drug Transport and Resistance Mechanisms
- Cancer Cells and Metastasis
- Fibroblast Growth Factor Research
- HER2/EGFR in Cancer Research
- Endometriosis Research and Treatment
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Pancreatic and Hepatic Oncology Research
- PI3K/AKT/mTOR signaling in cancer
- Ovarian function and disorders
- Cancer therapeutics and mechanisms
- Epigenetics and DNA Methylation
- Prostate Cancer Treatment and Research
- Ovarian cancer diagnosis and treatment
- Kruppel-like factors research
- Cancer-related Molecular Pathways
- Glycosylation and Glycoproteins Research
- Neuropeptides and Animal Physiology
- Reproductive System and Pregnancy
- Reproductive Biology and Fertility
- Cancer, Stress, Anesthesia, and Immune Response
- Breast Cancer Treatment Studies
- Cancer Treatment and Pharmacology
- Cancer, Lipids, and Metabolism
- Wnt/β-catenin signaling in development and cancer
Consejo Nacional de Investigaciones Científicas y Técnicas
2016-2025
University of Buenos Aires
2018-2024
Experimental Medicine and Biology Institute
2013-2021
Ospedale Policlinico San Martino
2020
München Klinik Bogenhausen
2020
University of Genoa
2020
Superconducting and other Innovative Materials and Devices Institute
2020
National Cancer Institute
2016
Center for Cancer Research
2016
University of California, San Diego
2016
Abstract The purpose of this study was to elucidate the mechanisms associated with specific effects AKT1 and AKT2 isoforms in breast cancer progression. We modulated abundance AKT IBH-6 T47D human cell lines showed that promoted proliferation, through S6 cyclin D1 upregulation, but it inhibited migration invasion β1-integrin focal adhesion kinase (FAK) downregulation. In contrast, F-actin vimentin induction. Thus, while overexpression local tumor growth, downregulation or peritumoral lung...
Is ceramide-1-phosphate (C1P) an ovarian protective agent during alkylating chemotherapy?Local administration of C1P drastically reduces damage induced by cyclophosphamide (Cy) via protection follicular reserve, restoration hormone levels, inhibition apoptosis and improvement stromal vasculature, while protecting fertility, oocyte quality uterine morphology.Cancer-directed therapies cause accelerated loss reserve lead to premature failure (POF). Previous studies have demonstrated that...
Background: Compelling evidence shows that progestins regulate breast cancer growth. Using preclinical models, we demonstrated antiprogestins are inhibitory when the level of progesterone receptor isoform A (PR-A) is higher than B (PR-B) and they might stimulate growth PR-B predominant. The aims this study were to investigate ex vivo responses mifepristone (MFP) in carcinomas with different PR ratios examine their clinical molecular characteristics. Methods: We performed human tissue culture...
624 Background: Biliary tract cancer (BTC) are rare tumors with a poor prognosis.Multidrug resistance protein 4 (MRP4) is transmembrane efflux transporter that influences on cellular growth and differentiation. Recent studies have linked high MRP4 levels to increased aggressiveness in pancreatic other tumor types. This study aimed evaluate the role of BTC its association clinical outcomes real-world cohort from endemic areas Argentina. Methods: We conducted retrospective analysis expression...
There is emerging interest in understanding the role of progesterone receptors (PRs) breast cancer. The aim this study was to investigate proliferative effect progestins and antiprogestins depending on relative expression A (PRA) B (PRB) isoforms PR. In mifepristone (MFP)‐resistant murine carcinomas antiprogestin responsiveness restored by re‐expressing PRA using demethylating agents histone deacetylase inhibitors. Consistently, two human cancer xenograft models, one manipulated overexpress...
// Sabrina Copsel 1,2 , Ariana Bruzzone 1 Maria May Julien Beyrath 3 Victoria Wargon Jeannette Cany 4 Frans G.M. Russel Carina Shayo and Carlos Davio 2 Instituto de Biología y Medicina Experimental-CONICET, Buenos Aires, Argentina Laboratorio Farmacología Receptores, Facultad Farmacia Bioquímica, Universidad Department of Pharmacology Toxicology, Radboud University Medical Center, Nijmegen, The Netherlands Laboratory Medicine, Correspondence: Copsel, email: Keywords :...
Improved efficacy of neoadjuvant endocrine-targeting therapies in luminal breast carcinomas could be achieved with optimal use pathway targeting agents. In a mouse model ductal carcinoma we identify tumor regressive stromal reaction that is induced by endocrine therapy. This reparative characterized neovascularization accompanied infiltration immune cells and carcinoma-associated fibroblasts stain for phosphorylated ribosomal protein S6 (pS6), downstream the PI3K/Akt/mTOR pathway. While...
Glycosylation-dependent programs control intestinal inflammation.
Background: Endocrine resistance and metastatic dissemination comprise major clinical challenges for breast cancer treatment. The fibroblast growth factor receptor family (FGFR) consists of four tyrosine kinase transmembrane receptors, involved in key biological processes. Genomic alterations FGFR have been identified advanced thus, are an attractive therapeutic target. However, the efficacy inhibitors on vivo tumor is still controversial. Objective: purpose this study was to evaluate role...
Abstract Recent findings show that MRP4 is critical for pancreatic ductal adenocarcinoma (PDAC) cell proliferation. Nevertheless, the significance of protein levels and function in PDAC progression still unclear. The aim this study was to determine role tumor aggressiveness. Bioinformatic studies revealed samples higher transcript compared normal adjacent tissue circulating cells express than primary tumors. Also, high are typical high-grade lines associate with an epithelial-mesenchymal...
// Gonzalo Sequeira 1,* , Silvia I Vanzulli 2,* Paola Rojas 1 Caroline Lamb Lucas Colombo 3 María May Alfredo Molinolo 4 and Claudia Lanari Institute of Experimental Biology Medicine, IBYME-CONICET, Buenos Aires, Argentina. 2 National Academy Instituto Roffo, Oral Pharyngeal Cancer Branch, NIDCR, NIH, Bethesda, USA. * Both authors had equal participation Correspondence: Lanari, email: Keywords : breast cancer, PR isoforms, doxorubicin, paclitaxel, mifepristone, mammary carcinomas,...
The basic component of Silastic® glue (Dow Corning) used to prepare pellets is polydimethylsiloxane. This compound also present in other commercial adhesives such as FASTIX® (Akapol SA) that are available any store for category. In the study we developed low cost, easy handmade steroid (HMSP) by mixing 17β-estradiol, progesterone or synthetic steroids with adhesive. We assessed serum levels progesterone, prolactin and luteinizing hormone ovariectomized mice treated 24 48 h 7, 14 28 days 20...
Abstract Progesterone receptors (PR) are prognostic and predictive biomarkers in hormone‐dependent cancers. Two main PR isoforms have been described, PRB PRA, that differ only has 164 extra N‐terminal amino acids. It reported several antibodies empirically exclusively recognize PRA formalin‐fixed paraffin‐embedded (FFPE) tissues. To confirm these findings, we used human breast cancer xenograft models, T47D‐YA ‐YB cells expressing or PRB, respectively, MDA‐MB‐231 modified to synthesize...
The xenobiotic transporter ABCC4 /MRP4 is highly expressed in pancreatic ductal adenocarcinoma (PDAC) and correlates with a more aggressive phenotype metastatic propensity. Here, we show that promotes epithelial-mesenchymal transition (EMT) PDAC, hallmark process involving the acquisition of mesenchymal traits by epithelial cells, enhanced cell motility, chemoresistance. Modulation levels PANC-1 BxPC-3 lines resulted dysregulation genes present EMT signature. Bioinformatic analysis on...
Can the bioactive lipid sphingosine-1 phosphate (S1P) act as an endothelial barrier-enhancing molecule and, in turn, restore vascular integrity and homoeostasis a rat model of ovarian hyperstimulation syndrome (OHSS).In vivo administration S1P may prevent early onset OHSS decrease its severity.Although advances prediction treatment have been made, complete prevention has not possible yet. follicular fluid from women at risk developing are lower comparison who such decreases capillary...
Ovarian cancer is the fifth leading cause of cancer‐related deaths in women. In past 20 years, canonical types drugs used to treat ovarian have not been replaced and survival rates changed. These facts show clear need find new therapeutic strategies for this illness. Thus, aim present study was investigate effect a gamma‐secretase inhibitor (DAPT) combination with Platelet‐derived growth factor B (PDGFB) on an xenograft model. To achieve goal, we analyzed administration DAPT alone...
Ulipristal acetate (UPA) is a selective progesterone receptor modulator used for emergency contraception that has proven to be highly effective in preventing pregnancy when taken up 120 h after unprotected sexual intercourse. Even though it may act mainly by delaying or inhibiting ovulation, additional effects of UPA on post-fertilization events cannot excluded. Therefore, the aim this study was determine whether single post-ovulatory dose could prevent using mouse as pre-clinical model....
To deeply understand the role of antibodies in context Trypanosoma cruzi infection, we decided to characterize A2R1, a parasite antibody selected from single-chain variable fragment (scFv) phage display libraries constructed B cells chronic Chagas heart disease patients. Immunoblot, ELISA, cytometry, immunofluorescence and immunohistochemical assays were used A2R1 reactivity. identify target, performed an immunoprecipitation two-dimensional electrophoresis coupled mass spectrometry confirmed...