A5050377577- Food Allergy and Anaphylaxis Research
- Allergic Rhinitis and Sensitization
- Eosinophilic Esophagitis
- Galectins and Cancer Biology
- Asthma and respiratory diseases
- Inflammatory Bowel Disease
- Clinical Nutrition and Gastroenterology
- Protein Hydrolysis and Bioactive Peptides
- SARS-CoV-2 and COVID-19 Research
- Digestive system and related health
- Immune Cell Function and Interaction
- Helicobacter pylori-related gastroenterology studies
- Gastrointestinal disorders and treatments
- Immunodeficiency and Autoimmune Disorders
- Transgenic Plants and Applications
- IL-33, ST2, and ILC Pathways
- Celiac Disease Research and Management
- Inflammasome and immune disorders
- Immune Response and Inflammation
- Vaccine Coverage and Hesitancy
- Toxoplasma gondii Research Studies
- T-cell and B-cell Immunology
- Contact Dermatitis and Allergies
- Probiotics and Fermented Foods
- Extracellular vesicles in disease
Universidad Nacional de La Plata
2015-2024
Consejo Nacional de Investigaciones Científicas y Técnicas
2015-2024
Comisión de Investigaciones Científicas
2021-2023
Centro Científico Tecnológico - Tucumán
2023
Fundación Ciencias Exactas y Naturales
2022
University of Buenos Aires
2012-2022
Unidad Ejecutora Lillo
2020
Ministerio de Salud - Provincia de Buenos Aires
2020
Secretaria de Salud
2020
Instituto de Física La Plata
2019
Docena GH, Fernandez R, Chirdo FG, Fossati CA. Identification of casein as the major allergenic and antigenic protein cow's milk. The objective this study was to analyze both allergenicity immunogenicity milk proteins. To end, 80 milk‐atopic patients were selected on basis presence milk‐specific IgE antibodies in serum compatible clinical history. Fifteen allergic other allergens 10 nonatopic subjects studied controls. specificity IgG determined by immunoblotting, employing components, i.e.,...
Massive vaccination offers great promise for halting the global COVID-19 pandemic. However, limited supply and uneven vaccine distribution create an urgent need to optimize strategies. We evaluate SARS-CoV-2-specific antibody responses after Sputnik V of healthcare workers in Argentina, measuring IgG anti-spike titers neutralizing capacity one two doses a cohort naive or previously infected volunteers. By 21 days receiving first dose vaccine, 94% participants develop spike-specific...
Inflammatory bowel disease (IBD) is a major source of morbidity in children and adults. Its incidence rising, particularly young people. IBD carries lifelong risk cancer, which proportional to duration. Drug surgical treatments rarely offer cure often carry high side effect burden. Dietary therapy highly effective Crohn's disease. For these reasons, there much interest developing novel dietary IBD. Curcumin, component the spice turmeric, an anti-inflammatory anti-cancer agent, shows...
Heterologous vaccination against coronavirus disease 2019 (COVID-19) provides a rational strategy to rapidly increase coverage in many regions of the world. Although data regarding messenger RNA (mRNA) and ChAdOx1 vaccine combinations are available, there is limited information about combination these platforms with other vaccines widely used developing countries, such as BBIBP-CorV Sputnik V. Here, we assess immunogenicity reactogenicity 15 1,314 participants. We evaluate immunoglobulin G...
The induction of peripheral tolerance may constitute a disease-modifying treatment for allergic patients. We studied how oral immunotherapy (OIT) with milk proteins controlled allergy in sensitized mice (cholera toxin plus proteins) upon exposure to the allergen. Symptoms were alleviated, skin test was negativized, serum specific IgE and IgG1 abrogated, substantial reduction secretion IL-5 IL-13 by antigen-stimulated spleen cells observed, while gene expression jejunum down-regulated, IL-10...
Soy-based formulas are the most employed cow's milk substitutes in treatment of allergy our country. Since adverse reactions have been reported allergic patients as a consequence exposure to soy proteins, we investigated possible cross-reactivity between components from soybean and milk. A specific polyclonal antiserum casein monoclonal antibodies were used immunoblotting competitive ELISA studies identify 30-kD component that cross-reacts with caseins. Its IgE binding capacity was tested by...
Summary Crohn's disease and ulcerative colitis are inflammatory bowel diseases (IBD) characterized by chronic relapsing mucosal inflammation. Tumour necrosis factor (TNF)-α, a known agonist of the mitogen-activated protein kinase (MAPK) pathway, is key cytokine in this process. We aimed first to determine whether p38 MAPK activated IBD inflamed mucosa, then studied effect four different p38α inhibitory compounds on phosphorylation secretion proinflammatory cytokines lamina propria...
Summary Background The Sabin vaccine is used world‐wide, and most children with food allergies receive it without incident. However, in the 2009 vaccination campaign conducted Argentina, four experienced immediate‐type hypersensitivity reactions following vaccination. Objective We aimed to review medical history of affected children, study their allergic condition after episodes analyse presence allergenic components. Methods Patients were selected based on immediate They assessed for cow's...
Abstract Background Therapeutic tolerance restoration has been proven to modify food allergy in patients and animal models although sublingual immunotherapy ( SLIT ) showed promise, combined therapy may be necessary achieve a strong long‐term tolerance. Aims In this work, we with systemic administration of IL ‐2 associated an anti‐ monoclonal antibody ‐2/anti‐ ‐2Ab complex or ‐2C) reverse the IgE‐mediated experimental allergy. Materials Methods Balb/c mice were sensitized cholera toxin milk...
Proteases play an essential role in the pathophysiology of inflammatory bowel disease (IBD), contributing to intestinal mucosal lesions through degradation extracellular matrix and alteration barrier function. Ulcerative colitis (UC) is characterized by extensive infiltrate neutrophils into mucosa hence, increased proteolytic activity. Human neutrophil elastase (HNE) a serine protease that has been reported be UC patients' mucosa. Based on our previous studies, we hypothesized HNE might...
<i>Background:</i> Cow’s milk allergy (CMA) is an important problem worldwide and the development of in vivo system to study new immunotherapeutic strategies interest. Intolerance soybean formula has been described CMA patients, but it not fully understood. In this work, we used a food model BALB/c mice cross-reactivity between cow’s protein (CMP) soy proteins (SP). <i>Methods:</i> Mice were orally sensitized with cholera toxin CMP, then challenged CMP or SP induce...
Abstract Inflammatory bowel diseases (IBD) are chronic and relapsing inflammatory conditions of the gastrointestinal tract including Crohn's disease (CD) ulcerative colitis (UC). Galectins, defined by shared consensus amino acid sequence affinity for β‐galactosides, critical modulators response. However, relevance galectin network in pathogenesis human IBD has not yet been explored. Here, we analyzed expression relevant members family intestinal biopsies, identified their contribution as...
Ulcerative colitis and Crohn’s disease, the two main forms of inflammatory bowel disease (IBD), are immunologically mediated disorders. Several therapies focused on activated T cells as key targets. Although Lactobacillus kefiri has shown anti-inflammatory effects in animal models, few studies were done using human mucosal cells. The aim this work was to investigate immunomodulatory bacterium intestinal from patients with active IBD. Mucosal biopsies surgical samples IBD adult ( n = 19) or...
Background: This study aimed the to investigate presence of residual allergenic cow's milk proteins (CMP) in some substitutes employed treatment allergy (CMA). These allergens may interfere with treatment, and elicit allergic reactions sensitized individuals. Methods: The protein composition different extracts was evaluated by Lowry's method tricine SDS‐PAGE. Different immunoenzymatic methods were used (ELISA, EAST immunoblotting) quantify total serum IgE specific IgE, as well detect...
Background Cross-reactivity between soybean allergens and bovine caseins has been previously reported. In this study we aimed to map epitopes of the major allergen Gly m 5 that are co-recognized by casein specific antibodies, identify a peptide responsible for cross-reactivity. Methods Cow's milk protein (CMP)-specific antibodies were used in different immunoassays (immunoblotting, ELISA, ELISA inhibition test) evaluate vitro recognition proteins (SP). Recombinant (α), truncated fragment...